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competentce cell, cloning - (Aug/15/2011 )

how do i make more competence cell from my E.coli BL21(DE3) competent cell? by growong up and glycerol stock? for plasmid transformation and protein expression proposal...

-Yilmaz Kaya-

As usual, grow it, and use Calcium chloride method or others as shown:

Hope this helps.

-Adrian K-

Thanks Adrian...

-Yilmaz Kaya-