Cell plating - (Sep/18/2012 )
You took the average of the 4 big (primary) squares? And the concentration of your stock is 6.75 x 105 or106 ....cells/ml? (for hemocytometer counting, here’s a good primer: http://www.microbehu...unting-chamber/)
If you got your count right, then you can just use this straightforward formula:
You already know your initial stock concentration (C1), your final conc C2 is 3.5 x 104/ml, V2 is 12 ml assuming that you would put 1 ml in each well so you can easily calculate how much of the stock you need to add to a final volume of 12 ml.
Thank you cassandra..:-)