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Cell Related Discussions
  1. ¿must treatment be aplied 24h post seed the cells? - (reply: 1)
  2. Question about transfecting siRNA into cells - (reply: 1)
  3. Mammalian Cell Lysis - (reply: 1)
  4. 293T cells for lentivirus production - (reply: 1)
  5. Counting and seeding cells?]. - (reply: 1)
  6. propagating commercial chemically competent cells - (reply: 2)
  7. CRISPR-Cas9 / insert a gene in cell line genome - (reply: 1)
  8. Beginner Cell and Tissue, and Molecular Biology methods reading list - (reply: 1)
  9. how to present Michaelis-Menten equation in cell designer - (reply: 1)
  10. TCDD (2,3,7,8-​Tetrachlorodibenzo-p-dioxin solution) cell line treatment - (reply: 1)
  11. cell invasion assay - (reply: 1)
  12. Help with the Red Blood Cell Lysis - (reply: 1)
  13. gene expression in cultured cell lines - (reply: 1)
  14. Splitting cells without centrifugation - (reply: 4)
  15. Are HEK293 cells a positive control for wild type p53 expression? - (reply: 1)
  16. Culturing LoVo cells - (reply: 1)
  17. Black dots contaminant in stable cell lines - antibiotics - (reply: 1)
  18. cell viability assay for caco-2 and pbmc - (reply: 1)
  19. 3T3 L1 cell culture - (reply: 1)
  20. Why my bacteria cells look weird in DAPI fluorescence? - (reply: 2)
  21. B & T cell activation in autoimmune disorders - (reply: 1)
  22. lentiviral transduction in BLCL cells - (reply: 3)
  23. Synchronization of HEK293T cells for IP - (reply: 2)
  24. Alternate cell line not giving same results as first - (reply: 1)
  25. Cell volume and reciprocal cell volume? Whaaat? - (reply: 1)
  26. How to detect specific-protein producing cells? - (reply: 8)
  27. Who came up with 50-100 IU/mL Antibiotic working concentration in cell culture? - (reply: 2)
  28. Help! Antibiotic and Serum Concentration in Cell Culture Media - (reply: 1)
  29. Large-scale cell growth and protein expression - (reply: 1)
  30. is cell signaling difficult? - (reply: 3)
  31. What would be the shortest and optimal method of extracting human cells for PCR? - (reply: 2)
  32. The adipose cell culturing - (reply: 2)
  33. Recommendation of cell signaling inhibitors - (reply: 2)
  34. Recovering cells from ancient sample - (reply: 3)
  35. Microinjection of cells into the lumen of spheroids ? - (reply: 1)
  36. Human cell membrane protein Western blot! - (reply: 2)
  37. Cell Line confluence level - (reply: 1)
  38. too much cell density can influence adhesion to culture plate - (reply: 1)
  39. Shipment conditions for cultured cells - (reply: 3)
  40. pIKK antibody for western blot (RAW 264.7 cells) - any recommendations? - (reply: 1)
  41. E-Cadherin Expression in OAW-42 Cells - (reply: 1)
  42. Why HepG2 cells looked like that? - (reply: 2)
  43. Trouble transfecting HEK 293 T and HUH 7 cells - (reply: 1)
  44. 3T3-L1 cell contamination - (reply: 3)
  45. Will lipofection of DNA work in post mitotic cells - (reply: 1)
  46. Cell death determination - (reply: 2)
  47. Culture Tubes for CHO cells - (reply: 1)
  48. SHSH5Y cells - (reply: 1)
  49. Is the mycoplasma that infects cell culture dangerous to humans? - (reply: 1)
  50. Distinguishing two populations of cells by TEM - (reply: 4)
  51. transfection of suspended CHO cells - (reply: 2)
  52. using IC50 to determine dosing of inhibitor in cell culture - (reply: 2)
  53. Software for automated cell counting? - (reply: 2)
  54. why is my cells dying in the centre of the wells after transfections - (reply: 1)
  55. How to establish drug-resistant cell lines? - (reply: 1)
  56. Are normal or immortalized human epithelial cells clonogenic? - (reply: 1)
  57. BJAB cells: does anyone have a specific protocol for transfection? - (reply: 1)
  58. Fibroblast Cell Culture Issue - Cells grouping together and cell clusters observ - (reply: 3)
  59. pUC and pSC101 vectors in the same cell - (reply: 3)
  60. Jurkat cell - normal receptor/gene expressivity - (reply: 1)
  61. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
  62. Inactivating effects on immune cells caused by mutation in the gene encoding the - (reply: 2)
  63. Is cultured cell reuse possible? - (reply: 1)
  64. Count cell bactria using haemacytometer - (reply: 1)
  65. Help with HUVEC cell transfection - (reply: 3)
  66. Cell clumps or contamination? Please help! - (reply: 1)
  67. How to clean up cell fragments before cell sorting - (reply: 1)
  68. Is any other method to maintain without changing media, or store cell culture fo - (reply: 2)
  69. MDA-kb2 cells - problem with luciferase expression - (reply: 6)
  70. Cell lysis method to get "intact" nuclear protein complex for CO-IP? - (reply: 1)
  71. Abnormality in PMA treated THP1 cells - (reply: 1)
  72. Preferred assay for measuring cytotoxicity or cell proliferation - (reply: 2)
  73. Cell lysis - (reply: 1)
  74. Can foam created when resuspending a cell pellet cause cell demage - (reply: 1)
  75. Distribution of cell receptors on the cell surface - (reply: 3)
  76. MTT Cell Treatment - (reply: 1)
  77. cells behaving differently - (reply: 4)
  78. Cell lines behaving differently to drugs - (reply: 2)
  79. How to achieve a uniform monolayer with HepG2 cells, seeded in 24 welll plate fo - (reply: 1)
  80. Efficient lysis of E.coli cells - (reply: 4)
  81. Alternative competent cells for QuikChange kit - (reply: 2)
  82. unknown bodies in cell culture - (reply: 1)
  83. Unbroken cell - (reply: 1)
  84. how to confirm lentiviral integration in a target cell line - (reply: 1)
  85. Invasion assay: analysis question: round cells - (reply: 2)
  86. Measuring ammonia in cell lysates - (reply: 1)
  87. Methanol oor PFA to fix cells for nuclear protien analysis by immunofluorescence - (reply: 4)
  88. What happens when trypsin is added to cells? - (reply: 1)
  89. Selective evolution of cells for cancer - (reply: 4)
  90. cell line - (reply: 3)
  91. I want know what companies are producing Biosimilar cell line - (reply: 1)
  92. contamination in HepG2 cell culture - (reply: 1)
  93. Which neuroblastoma cell lines grow the longest axons - (reply: 1)
  94. Help with Question for Cell Bio Take Home Exam - (reply: 2)
  95. leukemic cell lines 697 - (reply: 1)
  96. where do you get cells needed for your research - (reply: 1)
  97. PCR with mammalian cells as template - (reply: 1)
  98. Dislodge cell in 96well plate - (reply: 1)
  99. The right lentivirus plasmid for overexpression in human cells - (reply: 7)
  100. cell counting - (reply: 2)
  101. What do the glioma cell line prefixes LN and U stand for ? - (reply: 1)
  102. colorectal cell culture - (reply: 2)
  103. Easy to transfect primary cell line? - (reply: 1)
  104. Sample prep of cells for FACS analysis and running WB - (reply: 4)
  105. Functional states of the same cell versus Different cell types - (reply: 2)
  106. How long after transfection or dox induction of stable cell line before performi - (reply: 1)
  107. transfection of cells with two fluorescent reporters - (reply: 1)
  108. Stably transfected cells start dying - (reply: 6)
  109. U251 and U87 cells - (reply: 1)
  110. is -40 freezer enough for cell freezing? - (reply: 1)
  111. Experiment on nutrient surplus on Jurkat cell culture - (reply: 2)
  112. Help with HeLa cell cultivation - (reply: 5)
  113. skin lesion and low donor cell frequency in bone marrow transfer - (reply: 7)
  114. cell division rates - (reply: 6)
  115. Black dots-ghost cells or contamination? Please help - (reply: 6)
  116. Where do you purchase T cell hybridomas - (reply: 5)
  117. NK Cell Culture Selection and Expansion from PBMC - (reply: 2)
  118. 3D Cell Culture Matrix made of jelly / gelatin - (reply: 2)
  119. Cloning purified PCR product into cells - (reply: 5)
  120. Help ID fluoescently-labelled cells in the gut very much appreciated! - (reply: 2)
  121. Mention of Transgene Insertion into Cell Lines By Transfecting with cDNA - (reply: 2)
  122. Refreeze thawed cells? - (reply: 3)
  123. How can an animal cell obtain needed glucose when glucose is lacking in the diet - (reply: 2)
  124. Suppose a microorganism invades a cell, causing damage to ATP synthase. How woul - (reply: 3)
  125. How to capture live cells under microscope - (reply: 3)
  126. Identification of cells and tissue - (reply: 2)
  127. Research topic related to jurkat cell line - (reply: 3)
  128. What DCH5a cells are? / pCAMBIA1301 isolation from DHC5a cells - (reply: 3)
  129. When is it suitable for using NIH3T3 cells to validate cancer genes? - (reply: 1)
  130. Assays from very few number of cells - (reply: 1)
  131. What I have to do If my active compound showing toxicity in normal cells? - (reply: 1)
  132. Passaging cells - (reply: 1)
  133. Problem in mouse embryonic stem cell culture - (reply: 4)
  134. Is there always a presence of activated T cells in a healthy host? - (reply: 1)
  135. Bacterial contamination in cell culture - (reply: 3)
  136. Cell culture contamination - (reply: 1)
  137. Contamination or dead cells? - (reply: 1)
  138. How to choose cell line - (reply: 4)
  139. Urea effect on mice spleen cells - (reply: 3)
  140. Urea effect on mice spleen cells - (reply: 1)
  141. Trypan Blue if cells cultured in 96 well plate - (reply: 2)
  142. Best cryovials for liquid nitrogen storage of cells? - (reply: 2)
  143. Review articles on all types of mammalian cell culture - Any suggestion? - (reply: 1)
  144. 2 types of cell lines for RNA extraction - (reply: 1)
  145. live cell culture transport in 6 well plate - (reply: 6)
  146. Stem Cell Culture Theory - (reply: 4)
  147. Selectively arresting a subpopulation of biotinylated yeast cells. - (reply: 4)
  148. cell viability with PI staining - (reply: 2)
  149. Sonication of cells in ultrasound bath, without probe - (reply: 3)
  150. cell fractionation and microscopy - (reply: 3)
  151. Cell Cycle Analysis - crucial to keep cells cold after ethanol fixation, and aft - (reply: 1)
  152. cell lysis problem - (reply: 2)
  153. Made a mistake and fixed cells with 30% ethanol for cell cycle analysis. Can I s - (reply: 1)
  154. Thawing cells - (reply: 2)
  155. the same construct, two stable cell clones with different protein size - (reply: 2)
  156. Determine DNA sequence expression in cell line - (reply: 1)
  157. Harvesting cells - (reply: 3)
  158. Cell counters - Beckman Coulter Z1 counter - (reply: 1)
  159. Competent cells with Hanahan's method - (reply: 2)
  160. problem with cell synchronization - (reply: 4)
  161. can anyone tell me what kind of cell this is? - (reply: 3)
  162. Transfection of SH-SY5Y cells - (reply: 4)
  163. Protocol for isolating cells from Subcutaneous Tumors ? - (reply: 1)
  164. Hybridoma cells contaminated with large round cells! - (reply: 1)
  165. protocol for cell syncronization using colchicine - (reply: 2)
  166. PBMC cell stimulation? - (reply: 1)
  167. Caco-2 cells with unidentified contaminant - (reply: 2)
  168. MIN6 Cell Culture Contamination Risk - (reply: 1)
  169. Concentrating bacterial cells - (reply: 2)
  170. Would preserved cells for flow cytometry make your life easier? - (reply: 1)
  171. measure of cell size - (reply: 1)
  172. Transfecting cells for cell cycle analysis, how to deal with cell density? - (reply: 1)
  173. Splitting Cells that are shaped as small spheres - (reply: 2)
  174. Cells counting problem. - (reply: 2)
  175. Cell counts for Clonogenic and MTT assay - (reply: 3)
  176. Do I need to use untreated TC flask for suspension cell culture - (reply: 5)
  177. Cell cycle determination - (reply: 1)
  178. After centrifuge in serum-free media, forming cell sheet, instead of pellet in t - (reply: 2)
  179. Can cells be exposed to nanoparticles with ethanol as solvent??? - (reply: 2)
  180. Question about growth media for A20 Cell Line - (reply: 1)
  181. NSO cell culture - (reply: 1)
  182. Some queries regarding A549 cell culture - (reply: 1)
  183. Has anyone experience in isolating single-cell clones of CHO transfected cell li - (reply: 4)
  184. how to transport stem cells in suspension? - (reply: 2)
  185. animal cell culture - (reply: 1)
  186. help with contamination of cell culture - (reply: 2)
  187. Methanol concentration for cells? - (reply: 4)
  188. What happened to my cell? (Colony formation) - (reply: 7)
  189. problem of cell lysate - (reply: 3)
  190. Cell lysis and DNA quantification - (reply: 1)
  191. Best western loading control for comparing different cell lines - (reply: 2)
  192. B cell line - cell colour change and visible cell death - (reply: 1)
  193. Keeping apoptotic/detached cells for WB? - (reply: 3)
  194. Effective inhibitor of cell proliferation - (reply: 4)
  195. Lentivirus transfection using RAW 264.7 cells - (reply: 2)
  196. Neural Stem Cells; does it mean adult or embryonic? - (reply: 1)
  197. Cell culture contamination or not? - (reply: 1)
  198. orthotopic mouse model of U251 cell line - (reply: 4)
  199. How to release the bacterial cells from filter paper? - (reply: 4)
  200. Cell adhesion/detachment - (reply: 1)
  201. methylation observation in normal cell line - (reply: 1)
  202. Stem cells created by dipping researchers into coffee - (reply: 2)
  203. Freezing yeast cells - (reply: 4)
  204. codon optimization for CHO cells - (reply: 2)
  205. FITC conjugated phalloidin staining of cells on microcarrier beads - (reply: 1)
  206. chk effect of cell line genes on normal dna - (reply: 1)
  207. Would someone help me discuss what work can I do with laryngeal cancer cell hep2 - (reply: 12)
  208. DAPI stainged dots only in cells transfected with Fugene6 - (reply: 5)
  209. 293T cell transfection troubles - Lipofecamine 2000 - (reply: 3)
  210. Can I make my own electrocompetent Stbl4 cells? - (reply: 2)
  211. CHIP on frozen cell pellet - (reply: 1)
  212. UV Light benefits and costs on Cells - (reply: 2)
  213. Adapting a Drosophila cell culture to Zebrafish for subsequent MACS-sorting - (reply: 3)
  214. mouse embryonic stem cell culture on gelatine coated plates - (reply: 1)
  215. easiest cell lines to use - (reply: 4)
  216. Jurkat cell - (reply: 4)
  217. Mesenchymal stem cells Expressing BDNF - (reply: 5)
  218. PI staining cell cycle analysis - (reply: 1)
  219. Protein Purification from mammalian cells - (reply: 1)
  220. How to identify CD8 T cell receptor for a particular antigen - (reply: 2)
  221. How to inactivate H1N1 virus in Cell culture supernatant ? - (reply: 3)
  222. HaCaT cell line - not responding to inflammatory stimuli such as LPS/TNF-alpha - (reply: 4)
  223. Homogenous Cell Lysates - (reply: 1)
  224. Does anyone irradiate their cells? - (reply: 3)
  225. need help for transfection to synchronized cells - (reply: 4)
  226. If MCF-7 cell line can expresses Her-2 gene? - (reply: 5)
  227. What should I suspend my cells in prior to cytometry? - (reply: 2)
  228. Checking for efficieny competent cells - (reply: 1)
  229. Video on cell culture technique (broad brush) - (reply: 2)
  230. Setting up two drug kill curve for selection of stable cells - (reply: 1)
  231. No expression in stable transfected cell line - (reply: 2)
  232. Protocol for flow cytometry on mononuclear cells from mouse blood - (reply: 1)
  233. Cell line contamination? - (reply: 5)
  234. Inducing apoptosis by puromycin selection: toxic to the selected cells? - (reply: 1)
  235. Measuring cell proliferation from daily aliquots - (reply: 1)
  236. Cell culture of MYC immortalised HDFa - need advice - (reply: 1)
  237. Phoenix Amphoteric cell line to package lentivirus? - (reply: 6)
  238. miR30 based shRNA in mouse cells - (reply: 3)
  239. Cells are unhealthy - (reply: 2)
  240. Harvesting cells - (reply: 12)
  241. Dead cells after transfection - (reply: 9)
  242. preparation of cells for FACS analysis after transfection - (reply: 4)
  243. simultaneous knock down of two genes in a single cell line - (reply: 1)
  244. CoIP from cell lysate mix - (reply: 5)
  245. selective pressure on cells - (reply: 2)
  246. Conjugation of Ab to magnetic beads to isolate T cells from Spleen - (reply: 2)
  247. cell culture and biopsy - (reply: 1)
  248. Tiny black dots inside cell vacuoles - (reply: 1)
  249. I always have a cell depris floating in the supernatent of MDCK cells - (reply: 3)
  250. Is Trifluoroacetic acid toxic for cells? - (reply: 5)
  251. Treating suspension cells - (reply: 2)
  252. H9c2 cells..please help - (reply: 2)
  253. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
  254. How can i seed 2000 cell /well? (Using 200 microliter media and 96 plate, and as - (reply: 1)
  255. Establishing human neural stem cells - (reply: 3)
  256. Immunofluorescence Staining Problem of Mitotic Cells - (reply: 2)
  257. Cell Adhesion and Motility on Fibronectin vs Collagen - (reply: 1)
  258. FCS in Serum free medium for better Cell Adhesion? - (reply: 1)
  259. Possible to sort cells from formalin? - (reply: 1)
  260. 293s vs HEK/293 cells - (reply: 2)
  261. Cell culture medium contamination? - (reply: 1)
  262. What do sick 293t cells look like? - (reply: 3)
  263. H2DCFDA and fixation of cells before flow cytometry analysis - (reply: 4)
  264. Decreasing cell division - (reply: 1)
  265. Can I use methanol fixation to fix cells before proteomic analysis? - (reply: 1)
  266. Is it generally acceptable to ask another lab for cell line/plasmid/etc - (reply: 4)
  267. Do S2 cells normally have small 1-2um objects floating in culture? - (reply: 1)
  268. Problem in Cell Viewing - (reply: 3)
  269. How to view the encapsulated cells under microscope? - (reply: 5)
  270. Poor data quality using cultured cell in aCGH - (reply: 10)
  271. Immunohistochemical estrogen/progesterone receptor staining for CELLS - (reply: 1)
  272. Totipotent Stem Cell From Molecular Components - (reply: 5)
  273. How to count clumpy epithelial cells? - (reply: 5)
  274. Why cells are floating after incubation in EBSS?? - (reply: 1)
  275. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  276. Live cell sort DNA stain permeabilization - (reply: 1)
  277. Problem in bacterial cell culture - (reply: 12)
  278. Persistent contamination in cell culture - (reply: 9)
  279. PE and FITC stain after fixing cells with 4% PFA - (reply: 3)
  280. Adult stem cells - (reply: 2)
  281. Centrifuge stopped working while my blood samples were incubated in Cell lysis s - (reply: 1)
  282. Ways to Quickly Identify Antibiotic Resistance in Bacterial Cells (Non-tradition - (reply: 12)
  283. freezing competent cells without dry ice or liquid N - (reply: 3)
  284. Suspension cell line (RPMI) adhere to flask-WHY? - (reply: 1)
  285. Rapamycin Concentration for Cell Culture - (reply: 1)
  286. how to calculate cells and plate - (reply: 2)
  287. yeast conatamination in primary cells - (reply: 4)
  288. Maltodextrin will kill all the bacterial cells? - (reply: 5)
  289. Keratinocyte Culture: Cells detach - (reply: 7)
  290. Measure growth of plant cell culture - (reply: 1)
  291. Cell Lysate Western Blot Little or No Signal - (reply: 7)
  292. Cell line identity (HEK293-E) - (reply: 1)
  293. How do I calculate the enery absorved by cells form a UV light - (reply: 3)
  294. Immunocytochemistry - Washing away PC12 cells - (reply: 2)
  295. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
  296. Total Protein in COS-7 Cells - (reply: 1)
  297. protein extraction from cell nucleus - (reply: 2)
  298. Which gauge for proper shear force on certain cell dimension - (reply: 1)
  299. Mammalian Cell Lysis Buffer Triton-X100 Content - (reply: 6)
  300. DNA from frozed cells??? - (reply: 2)
  301. SH-SY5Y stable cell transfection and RA differentiation - (reply: 1)
  302. Tumor cell analysis - (reply: 2)
  303. What can I do with my MCF7 cells? - (reply: 1)
  304. Evolution of Living Cells from Non-living Organic Matter - (reply: 3)
  305. cell lines - (reply: 5)
  306. M2 antibody background in Xenopus cell extracts - (reply: 6)
  307. Asking for guidelines to start L6 cell line culturing - (reply: 1)
  308. how to separate organelles from mammalian cells - (reply: 1)
  309. Cell culture contamination ? - (reply: 5)
  310. How to coat 96 well plate with poly-L-Lysine for cell culture use? - (reply: 2)
  311. Is Protein A present on the Staphylococcus saprophyticus cell wall too? - (reply: 2)
  312. Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
  313. red dots in cell culture line? - (reply: 3)
  314. Integrity of cell monolayer - (reply: 1)
  315. problem with COV318 ovarian cancer cell line - (reply: 4)
  316. Expansion of commercial competent bacterial cell lines? - (reply: 2)
  317. atom replacement in human cells - (reply: 2)
  318. What stage of growth are the cells I counted? - (reply: 4)
  319. HeLa cell culture - (reply: 6)
  320. Lambda Phosphatase Treatment of Cell Lysate - (reply: 1)
  321. Problem in Determination of Total Bacterial Cells - (reply: 13)
  322. Preparing a cell culture stock to plate out at 7x10^5 per well? - (reply: 1)
  323. Cell culturing tips - (reply: 2)
  324. SAOS.2 Cell detachment without using Trypsin. - (reply: 3)
  325. Normal cells or contamination?! - (reply: 4)
  326. H2DCFDA for MDCK cells - (reply: 2)
  327. immortalized human neuronal cells - (reply: 4)
  328. LNCaP Cell Clusters - (reply: 2)
  329. Freezing viable cells in 96-well format? - (reply: 3)
  330. question about sequential transfection several plasmids in 293T and COS7 cells - (reply: 1)
  331. unidentified objects in my cells, no staining.. what are they? - (reply: 2)
  332. Does fixation and permeabilization of cells affect their oxidative stress levels - (reply: 1)
  333. HBE cell culture CRL-2079 MEDIA question - (reply: 1)
  334. Cells dying in culture after 24 hours - (reply: 6)
  335. SH-SY5Y cells: basics advice - (reply: 1)
  336. Cell clumping: dead cells? - (reply: 6)
  337. What is your favorite method for making chemically competent E.coli cells? - (reply: 7)
  338. growing cometent cells - (reply: 1)
  339. Where can I purchase HepG2.2.15 cell line - (reply: 1)
  340. RAW 264.7 cell line - (reply: 1)
  341. Mycoplasma infected cell line clean-up - (reply: 1)
  342. Rheumatoid Factor (RF) producing cell line? - (reply: 1)
  343. Weighing harvested cells - (reply: 1)
  344. Temperature Variation on Cell Growth Experiment - (reply: 1)
  345. Morphology of cultured cells - (reply: 5)
  346. Growing GH teratocarcinoma cell line- help needed - (reply: 2)
  347. Cells Detach After Transfection - (reply: 1)
  348. How to determine the location of integrated DNA in stable cell lines - (reply: 2)
  349. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
  350. When cell media becomes "used up", it acidifies - (reply: 3)
  351. Cell cycle analysis for SubG1 fraction - (reply: 3)
  352. Clone selection for scrambled shRNA transfected cells ? - (reply: 2)
  353. Cytokines feeding cell culture - (reply: 1)
  354. Experience with U-937 cell line - (reply: 3)
  355. Cell culture contamination issue - unable to identify the contamination - (reply: 1)
  356. cell confluency in IF - (reply: 2)
  357. Protein purification for adherent mammalian cells - (reply: 2)
  358. Antibody production in Expi293 cells - (reply: 2)
  359. death of transfected cells after first passage in G418 medium - (reply: 1)
  360. clonogenic assay for shRNA transfected cells - (reply: 3)
  361. Cell lysis in chromosome conformation capture - (reply: 2)
  362. Relationship between cell lines and cancer research (IHC) - (reply: 4)
  363. Serum starvation - something similar for serum-free kept cells ? - (reply: 2)
  364. Sodium bicarbonate addition to GMEM for BSR T7/5 cells - (reply: 2)
  365. No proteins after HeLa cell lysis - (reply: 3)
  366. How can i fix cultured cells on glass slides for insitu hybridization - (reply: 1)
  367. Blocking a protein in cell culture through antibody - (reply: 10)
  368. How to propagate Electro-competent DB3.1 cells - (reply: 6)
  369. Number of cell - (reply: 5)
  370. Difference between PFA, acetone and methanol in cell fixation - (reply: 1)
  371. cell proliferation statistics methods - (reply: 4)
  372. Losing cells during fixation - (reply: 2)
  373. ovarian cancer cell line: phenotype disappears - (reply: 3)
  374. Recombinant Plasmid is not being amplified in transformed cells - (reply: 2)
  375. HeLa Cells on Chin/in Nose? - (reply: 7)
  376. Can centrioles be seen when the cell is not undergoing cell division? - (reply: 3)
  377. How much ethanol (in micro g) has toxic effects on cells? - (reply: 1)
  378. penicillin/streptomycin in cell culture - (reply: 3)
  379. Recombinant expression of cytokines in mammalian cells without the propeptide re - (reply: 1)
  380. Cell lysis for unknown reasons - (reply: 1)
  381. Preparing cells for plating - (reply: 3)
  382. ChIP with low cell number - (reply: 2)
  383. Mammalian cell culture and G418 selection - (reply: 1)
  384. Losing cells during intracellular staining method! - (reply: 1)
  385. G418 mammalian cell selection question - (reply: 3)
  386. DIFFERENCES BETWEEN GLIAL AND GLIOMA CELL? - (reply: 4)
  387. Bought HEK293T cells from ATCC -- passage number question - (reply: 1)
  388. Cell lines selected with G418 but no overexpression of GOI - (reply: 3)
  389. cell pellet question - (reply: 2)
  390. B cells cultures. Immunoglobuline production - (reply: 1)
  391. ChIP problem - using cells as a negative control - (reply: 7)
  392. Blood cell migration from mother to fetus - (reply: 1)
  393. Qiagen mini prep cell fragment pellet - (reply: 2)
  394. Cell death detection using adenosin kinase - (reply: 5)
  395. THP-1 differentiation using PMA yielding black dots within cell structure - affe - (reply: 4)
  396. Transfecting three plasmids into hES cells - (reply: 2)
  397. Agglomeration of suspension cells (T-cells) - (reply: 1)
  398. Selection of E.coli competent cells - (reply: 2)
  399. Chelating Aluminum from cell culture - (reply: 5)
  400. chemotherapeutic-resistant cell lines - (reply: 1)
  401. infection assay-buffer that lyses mammalian cells but not bacteria - (reply: 1)
  402. HEK293 vs. SH-SY5Y Cell lines? Which best for in vitro Alzheimer's Model? - (reply: 2)
  403. Selection of needle size to homogenize cells for RNA extraction - (reply: 3)
  404. Electroporate lentiviral vector for stable cell line - (reply: 2)
  405. Place to buy (soluble) ligands for cell culture. - (reply: 3)
  406. NIH 3T3 cells cross contamination - (reply: 1)
  407. NIH 3T3 cells - (reply: 3)
  408. Cells adhering to walls of conical tube? - (reply: 7)
  409. PI cell cycle analysis and apoptosis - or would a different stain be better? - (reply: 2)
  410. choosing the correct subculturing ratio - ovarian cancer cell lines - (reply: 1)
  411. RAW 264.7 cell line - (reply: 4)
  412. washing of attached bacterial cells - (reply: 3)
  413. quick and non-expensive method of testing cross cell contamination? - (reply: 4)
  414. Cell Debris Isolation From Blood - (reply: 3)
  415. How to calculate drug amount to use in cell lines - (reply: 3)
  416. Growing certain number of bacterial cells in LB - (reply: 1)
  417. T cells or Lymphocytes? - (reply: 2)
  418. Western Blot with fixed cells - (reply: 5)
  419. Less than 10 GFP positive cells - (reply: 2)
  420. Doubt: Cell plating for different cell culture plate formats - (reply: 3)
  421. How to culture HK-2 cells - (reply: 6)
  422. competent cells - (reply: 1)
  423. Debris in cell culture - (reply: 2)
  424. What housekeeper to use with HEK cells? - (reply: 3)
  425. Housekeeper for HEK cells? - (reply: 1)
  426. Type I Collagen Vs. Poly-L-lysine Vs. Gelatin Coated coverslips (NSC34 cells) - (reply: 1)
  427. Cell-repellent coating / surfaces - Growing cells on beads - (reply: 2)
  428. C3HT101/2 Cell Line not attaching to T25 flask- nightmare!!!! - (reply: 5)
  429. Phorbol myristate acetate treatment of J774 cell line - (reply: 1)
  430. Gene expression profiling with small cell number - (reply: 1)
  431. Gene expression assay with small cell number - (reply: 3)
  432. Spontaneous immortalization of mouse cells in vitro - (reply: 1)
  433. Stem Cell Therapy comes of age? Discussion Topic - (reply: 1)
  434. cell free DNA and maintaining stability - (reply: 3)
  435. Freezing Cells For Use In Assays - (reply: 2)
  436. INS-1E cells migration assay - (reply: 1)
  437. Short-term storage of HEK293 cells before FACS - (reply: 3)
  438. Sample storage for ELISA (cell culture supernatant) - (reply: 2)
  439. Any tips on how to avoid lifting cells in 96well plates - (reply: 3)
  440. Primary cell line - (reply: 5)
  441. 2D Image Primary Cell line - (reply: 13)
  442. will mRNA from different cell line lead to loss of expected product in RT PCR? - (reply: 2)
  443. MCF-7 Clumping - (reply: 3)
  444. Geneticin Resistant Cell Lines and Stable Transfection - (reply: 1)
  445. To small amount of cells used for shRNA transfection - (reply: 1)
  446. using cell line to generate standard curve - (reply: 6)
  447. Autofluorescence in thick structure cluster of cells - (reply: 4)
  448. Primary culture of fibroblast cells from rat/mouse - (reply: 4)
  449. Serial dilution of cells - (reply: 3)
  450. Cloning repetitive sequences in mammalian cells - (reply: 3)
  451. Do cell signaling antibodies work with 1h RT incubation? - (reply: 2)
  452. Plant Cells - what are they? - (reply: 1)
  453. HS-5 and HS27A cells - (reply: 1)
  454. MC-3T3 cells are not mineralizing... - (reply: 3)
  455. HS-5 cell culture "question" - (reply: 1)
  456. Problem with Kasumi-6 cell line - (reply: 1)
  457. G418(Geneticin) is not killing my cells - (reply: 2)
  458. Community feedback needed to clarify terms for a Cell Culture Ontology - (reply: 1)
  459. Quantify total DNA in small cell lysate...? - (reply: 1)
  460. Trouble validating in multiple cell types - (reply: 1)
  461. Gating of dendritic cells - (reply: 7)
  462. Please help me take a look at my cells to see whether is it contaminated - (reply: 3)
  463. cell binding assay with nanoparticles? - (reply: 2)
  464. Cell lines for clonal selection - (reply: 3)
  465. Pelleting cells by gentle centrifugation for addition to media - (reply: 13)
  466. SH-SY5Y Immortalized cell lines; researching neurotoxicity? - (reply: 3)
  467. Pelleting bacterial cells - (reply: 1)
  468. How viable is cell culture for a model? - (reply: 2)
  469. Help with adherent cell culture - (reply: 7)
  470. study of receptors in cell lines - (reply: 1)
  471. Cell line nomenclature - suffixes ? - (reply: 3)
  472. Maintainence of transformed cells - (reply: 5)
  473. improving viability of pelleted cells - (reply: 2)
  474. Buying Ins-1e cells - (reply: 1)
  475. How do/should cell biologists calculate power of analysis? - (reply: 1)
  476. comparing ADCC effect in 3 cell lines. - (reply: 1)
  477. Help: Best way to disrupt blood cells to generate blood debris and gDNA to be us - (reply: 4)
  478. Ric3 NAChR 7 expression in HEK cells - (reply: 1)
  479. virus and cell surface receptor interaction - (reply: 4)
  480. Music Video on Cell Division/Mitosis - (reply: 3)
  481. How to select competent E. coli cells? - (reply: 7)
  482. Host cell protein sequence search - (reply: 1)
  483. qPCR from HEK cells transfected with Plasmid - (reply: 4)
  484. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
  485. Preparing supernatant from cell culture for western blot - (reply: 4)
  486. Fast/easy way to measure cell size - (reply: 4)
  487. Which is the best way to generate growth curves for cancer cell lines - (reply: 7)
  488. Cell number issue - Nile Red staining - (reply: 3)
  489. Music Video on Cell Division/Mitosis - (reply: 1)
  490. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
  491. Integrin B1 Staining in HT1080 cells - (reply: 1)
  492. delaying RNA extraction; using buffer RLT to disrupt cells and place on ice? - (reply: 3)
  493. My cells won't pellet - any ideas? - (reply: 6)
  494. Is it possible to induce signalling pathways activity in Hela cells using multip - (reply: 1)
  495. EMSA: cell extract vs purified protein - (reply: 2)
  496. Insect cells transfection reagent - (reply: 1)
  497. Low Protein Content In Cell Lysate - (reply: 1)
  498. Can you see GFP after lysis mammalian cells? - (reply: 5)
  499. Centrifugation speeds for cells. - (reply: 8)
  500. Cell recovery from ethanol fixation - (reply: 4)
  501. Cryopreservation of MSC at lower cell density - (reply: 2)
  502. C-terminal GST fusion problem in mammalian cell - (reply: 6)
  503. Identify cell culture contamination - (reply: 2)
  504. Adipose stem cell culture - (reply: 5)
  505. Determine cell proliferation by viability assay ? - (reply: 6)
  506. Apoptosis via cell to cell contact - (reply: 1)
  507. Cells stop to grow - (reply: 6)
  508. Pore formation in cells after adding amphotericin B in culture medium - (reply: 2)
  509. Long Treatment, Rapidly Dividing Cells - (reply: 7)
  510. Need help calculating cell number to plate - (reply: 1)
  511. Injection of human cell culture conditioned media into nude mice - (reply: 1)
  512. How long can I store cell lysis buffer at 4C for? - (reply: 1)
  513. Heeeeeelp..cell cycle sample..is it possible to store it? - (reply: 2)
  514. Subculture issue of primary cell - (reply: 6)
  515. Differentiating mouse from human cells with PCR - (reply: 5)
  516. primary valve interstitial cells transfection - (reply: 1)
  517. Stem Cell Donation - (reply: 7)
  518. isolating DNA from cells in suspension - (reply: 5)
  519. Software for designing cell pathways - (reply: 1)
  520. Tamoxifen citrate solubility for cell work?? - (reply: 1)
  521. Centrifuged cells stick to wall of reaction tube - (reply: 12)
  522. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  523. Changing Genomic Regions in Host Cell - (reply: 1)
  524. How to transform adherent cells to suspension (CHO cells) - (reply: 2)
  525. my competent cell grow on any agar plate - (reply: 7)
  526. Bacterial Contamination in HuEmbryonic cell lines seen in SFM4 but not in DMEM + - (reply: 3)
  527. Cell attachement occuring after 48 hours, not 24 hours. - (reply: 3)
  528. Transfection of a non human gene into human cell line - (reply: 1)
  529. Procedures to replace vial of cells and passage number. - (reply: 4)
  530. Immunofluorescence on cells - can I keep the coverslips unstained in PFA? - (reply: 4)
  531. p14ARF and MDM2 in transformed cells - (reply: 1)
  532. RNA isolation with low number of cells (1*10^4 to 5*10^4) for RT-PCR - (reply: 3)
  533. contamination of human cell lines with yeast - (reply: 3)
  534. Generation of single cell suspensions from neural tissue - (reply: 2)
  535. Is this contamination or cell death? - (reply: 7)
  536. BaF3 cell culture in 96 well plates - (reply: 2)
  537. THP-1 differentiated cells detach when washing with PBS (Mg and Ca free PBS) - (reply: 2)
  538. unwanted cell lysis during LB+Amp incubation - (reply: 5)
  539. Cells spontaneously detaching? - (reply: 1)
  540. Immuno-staining for cell surface/membrane markers after permeabilisation - (reply: 4)
  541. different type of media for cell culture - (reply: 1)
  542. Influenza: cell and mechanism information? - (reply: 1)
  543. Can CMV promoter start transcription in prokaryote cells - (reply: 1)
  544. How to treat cells with TNFa - (reply: 2)
  545. CLOUDINESS of RIN5F cell culture - (reply: 7)
  546. U87MG cells not growing well - (reply: 3)
  547. Concentrating low volume of cells - (reply: 8)
  548. Preparing a conditioned medium from cancer cells - (reply: 3)
  549. Presence of melanin in melanin containing cell line - (reply: 1)
  550. Cancer cells culture - (reply: 1)
  551. How to achieve uniform cell distribution in culture vessle - (reply: 6)
  552. viability/integrity stain of fungal cells - (reply: 5)
  553. Cell culture contamination - (reply: 3)
  554. airway primary cells stop growing at passage 3 - (reply: 1)
  555. cell culture neural cells easier? - (reply: 6)
  556. Question - Corticosterone, cell culture - (reply: 1)
  557. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
  558. Where to obtain rat embryonic stem cells? - (reply: 3)
  559. Stable cell line - (reply: 1)
  560. PBMC cells handling - (reply: 1)
  561. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
  562. Unable to clone in E. coli cells - (reply: 3)
  563. removing MHC class I from the cell surface - (reply: 3)
  564. Osteosarcoma cell culture problem - (reply: 7)
  565. rtPCR stem cell signalling array from quiagen- has anybody experience? - (reply: 1)
  566. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 4)
  567. Thawed cells spent 4 hours extra in DMSO.. problem? - (reply: 1)
  568. colon cancer cell lines vs normal colon epithelial cells - (reply: 1)
  569. Cell count and seed, plz help... - (reply: 4)
  570. In and out of cell Help 2 - (reply: 6)
  571. In and out of cell Help - (reply: 2)
  572. Primary culture of breast tumor cells - (reply: 1)
  573. Strange Cell Morphology & Cell Debris Following Lentiviral Transduction - (reply: 2)
  574. Cell numbers versus confluency - (reply: 2)
  575. Flow cytometry in THP-1 cells - (reply: 1)
  576. Competent bacterial cells - (reply: 2)
  577. Could a change of FBS affect outcome of a cell proliferation assay? - (reply: 4)
  578. How do they make passage limited cells? - (reply: 1)
  579. transient transfection problem using ME180 cells - (reply: 1)
  580. How to completely remove cells from surface - (reply: 3)
  581. Hiperfect siRNA in HT-29 cells help - (reply: 2)
  582. Isolation of BOTH cells AND serums from mouse blood - (reply: 5)
  583. Transfection of pCI-neo plasmid into 3T3-L1 cells - (reply: 1)
  584. Commercial source Stomach Smooth Muscle Cell lysate - (reply: 1)
  585. DMEM or EMEM Does it matter? (HeLa Cells & Transfection) - (reply: 3)
  586. cell line contaminated by something... - (reply: 2)
  587. Flag Staining in U87 cells - (reply: 4)
  588. protein is not expessing in HEK 293 cells - (reply: 2)
  589. competent cells: CaCl2 autoclave vs filter sterilisation - (reply: 12)
  590. HepG3 cell line origin - (reply: 3)
  591. dilution of cell suspension - (reply: 4)
  592. how to preserve spleen cells for long duration, for flowcytometry - (reply: 2)
  593. DNA extraction for MCF-7 and HeLa - (reply: 4)
  594. Increasing cell growth rate in plain M9 medium - (reply: 1)
  595. Puromycin - shRNA - stable cell line for knock down - (reply: 4)
  596. Reducing cell adhesion - (reply: 1)
  597. The Hek 293T cell line looks like non adherent cells after to be sorted in FACS - (reply: 1)
  598. Hygromycin selection of virally-transduced cell lines - (reply: 1)
  599. Which cells express which genes? - (reply: 1)
  600. IGF1 stimulation of THP1 cells - (reply: 2)
  601. Cells detach during bodipy 493/503 staining - (reply: 1)
  602. RNA isolation from small numbers of cells - (reply: 6)
  603. Distinguish murine and human cells by surface staining? - (reply: 1)
  604. starting HaCaT cell line. basic questions - (reply: 6)
  605. culture media for pancreatic cancer stem cells - (reply: 2)
  606. Lysis method for single human embryonic cell - (reply: 5)
  607. In vitro transcription/translated protein to cells!!! - (reply: 1)
  608. Cell plating - (reply: 2)
  609. sample preparation from whole cell lysates for Edman degradation - (reply: 2)
  610. colony formation assay in A2780 cells - (reply: 4)
  611. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  612. Which is a better way to culture cells after thawing ? - (reply: 2)
  613. Comet assay sperm cells - (reply: 5)
  614. Non-specific bands different for same cell line, using same primary and secondar - (reply: 1)
  615. Live/Dead Staining of already Permeable Cells - (reply: 10)
  616. cell synchronisation for fibroblasts (help choosing method/chemical) - (reply: 2)
  617. Cell Therapies - (reply: 2)
  618. SURE E. coli competent cells - (reply: 1)
  619. Stable protein expression in Vero cells - (reply: 2)
  620. HL60 cell line suppliers - (reply: 1)
  621. Insoluble material floats over my SDS lysate of human cells after centrifugation - (reply: 7)
  622. Cell Fixation for DNA flow cytometry in fibroblasts - (reply: 2)
  623. Question about HeLa cell overconfluence - (reply: 3)
  624. 293T cell contamination post-transfection - (reply: 1)
  625. HEK293T cells are too fragile?? - (reply: 2)
  626. number of splitting after defrozing cells - (reply: 2)
  627. my human iPS cells have a lot of differentiation - (reply: 4)
  628. Properly designing a drug treatment assay measuring cell cycle arrest - (reply: 2)
  629. Detaching Breast Cancer cells (MCF-10A, HCC70) without trypsin- used for FACs - (reply: 4)
  630. counting cells before adding them to 96 well plate - (reply: 7)
  631. Cell Suspension - (reply: 2)
  632. What will happen if the cell culture medium is suddenly changed to another one? - (reply: 5)
  633. MDA-MB-231 cells undergoing senescence- help! - (reply: 2)
  634. Creating a stable cell line by transfecting a plasmid without any fluorescence t - (reply: 3)
  635. coating cover-slides before seeding cells for fluorescent microscopy - (reply: 4)
  636. How to induce early apoptosis in cells without using chemicals or UV - (reply: 5)
  637. choice of cell line for orthotopic xenograft implant - (reply: 3)
  638. Compound concentration depending on cell number? - (reply: 4)
  639. How to remove mycoplasma contamination in cell lines? - (reply: 5)
  640. [Question] How to remove cell debris stuck on the attached cells in primary card - (reply: 2)
  641. gene overexpression in cells: virus transduction or transfection following selec - (reply: 4)
  642. Reagent used to adjust pH of cell culture media - (reply: 3)
  643. Powder indomethacin in cell culture media?? - (reply: 2)
  644. How many generation of HEK293 cell can undergo? - (reply: 1)
  645. [Question] how to make primary-cultured cells evenly distributed? - (reply: 11)
  646. small dots in cell culture - (reply: 2)
  647. How delete survival gene in bacteria still keeping cell alive. - (reply: 5)
  648. Clumpy competent cells - (reply: 3)
  649. Preparation of bacterial cells for SEM and TEM - (reply: 2)
  650. G418 advice in estab;lishing stable cell line - (reply: 1)
  651. Electroporation of J774.2 murine macrophage cell line - (reply: 1)
  652. About trypsinizing adherent cells in 96-well plates - (reply: 2)
  653. Passaging cells - (reply: 12)
  654. Preparing homemade XL1-blue cells in CCMB80 buffer - (reply: 1)
  655. Understanding Cell Passaging - (reply: 5)
  656. How to extract DNA from small number of cells - (reply: 2)
  657. How to identify beta pancreatic cells in dish of heterogenous cells? - (reply: 12)
  658. Cell Splitting Ratio Question - (reply: 5)
  659. Sf9 cell suspension culture - (reply: 5)
  660. competent cell formation - (reply: 1)
  661. Help! Clontech Xfect is killing my T-Rex HeLa cells!!! Why! - (reply: 1)
  662. How long are cells viable on ice - (reply: 2)
  663. Discrepancy between tissue and cell culture - (reply: 1)
  664. What is the density of a human skin cell? - (reply: 8)
  665. Determining the % of GFP/CHERRY cells in co-mixed culture - (reply: 3)
  666. Is the genomic DNA similar among different cell lines? - (reply: 4)
  667. serumm free culture of adherent cho cells - (reply: 1)
  668. detection of mesenchymal stem cells in cancer tissue sample - (reply: 1)
  669. Can u suggest any myoepithelial cell isolation protocol with the help of reagent - (reply: 1)
  670. cell line cultured in suspension - (reply: 1)
  671. GFP cells in paraffin.....orange? - (reply: 1)
  672. overexpression using pcDNA3.0 in stably knockdown cells - (reply: 3)
  673. Cell staining for fluorometric assay - (reply: 5)
  674. Would E. Coli cells be stable at room temp, if in media with glycerol? - (reply: 1)
  675. Long-Term Culturing of SH-SY5Y Cells - (reply: 1)
  676. N acetyl cysteine solution for cell culture - (reply: 3)
  677. Aortic smooth muscle cell contamination - (reply: 1)
  678. need recommendation for stable transduction of fibroblast cells - (reply: 1)
  679. Sub G1 in cell cycle - (reply: 2)
  680. DMSO induces cluster growing in LNCaP cells? - (reply: 1)
  681. Cell cycle arrest - adherent cells going into suspension - (reply: 4)
  682. Prevent adherent cells from adhering - (reply: 3)
  683. INS-1E cells not adhere to the flask! - (reply: 1)
  684. A549 cells are not attaching to flask - (reply: 2)
  685. irradiated J774 cells - (reply: 1)
  686. Cell lysis with proteinase k - (reply: 2)
  687. One siRNA sequence, many cell lines - (reply: 1)
  688. Freezing cell lysates for westerns - (reply: 2)
  689. SDS-PAGE for cell culture medium - sample preparation - (reply: 1)
  690. adherence to host cell question - (reply: 2)
  691. New patch of sf9 cells not growing - (reply: 2)
  692. contamination in yeast cell culture - (reply: 8)
  693. Tissue culture contamination like bacteria inside the cells. What is this kind o - (reply: 1)
  694. sf9 Insect cells questions - (reply: 3)
  695. Kit to mesure cell death - (reply: 4)
  696. An improve method for making Competent Cells without heat shock. How? - (reply: 2)
  697. J774 and Flow cytometry....are they really big cells ? - (reply: 9)
  698. immunostaining cells grown in matrigel - (reply: 1)
  699. Cell concentration for flow cytometry - (reply: 2)
  700. Transient Transfection: Adherent vs. Suspended/Floating Cells - (reply: 7)
  701. Cells growing very slowly - (reply: 6)
  702. SH-SY5Y cells do not grow - (reply: 1)
  703. GH3 cells - (reply: 4)
  704. The connection between LPS and PMA stimulation for maturation of ThP1 cells - (reply: 2)
  705. Using normal competent cells in electroporation method? - (reply: 1)
  706. Washing n2a cells out of 75 cm flask? - (reply: 1)
  707. How to dissociate N2A cells after centrifugation? - (reply: 3)
  708. Undefinable particles in cell culture flask - (reply: 2)
  709. Help: transduction of primary blood cells (T): lentiviral vs. adenoviral vector - (reply: 1)
  710. immunostaining of cells in culture - (reply: 1)
  711. healthy stem cells vs cancer stem cells - (reply: 1)
  712. values for mRNA molecules per cell - (reply: 1)
  713. Help!! need to culture PC3M cell line. Any advice? - (reply: 5)
  714. MCF10A Tet-ON stable cell line generation - (reply: 1)
  715. Primary cell line - (reply: 2)
  716. Neurosphere Assay with neuroblastoma cell lines - (reply: 1)
  717. How to decontaminate adherent cells with gentamicin - (reply: 2)
  718. Contamination in CHO cell ? - (reply: 3)
  719. Thawing Hek 233t cells- but cells dying!! - (reply: 3)
  720. How to purify RNA from a handful number of cells? - (reply: 5)
  721. Check cell viablity after FACS - (reply: 2)
  722. ISH on fluorescing cells - (reply: 1)
  723. Please help, may i replenish Glutamine in cell culture medium? - (reply: 3)
  724. Protein extraction from dead cells - (reply: 1)
  725. Cells in suspension vs. plated - (reply: 2)
  726. PI stain for cell viability~~Compensation? Suggestion needed~~^^ - (reply: 5)
  727. Hepg2 cells growing on top of each other - (reply: 1)
  728. urgent! calculating concentration of compound with different cell amounts - (reply: 3)
  729. h9c2 cells culture - (reply: 5)
  730. Does anyone have experience of subjecting cultured cells to mechanical stress? - (reply: 1)
  731. Transformed cells have many mutations! pET-46 Ek/LIC + sequence verified ins - (reply: 1)
  732. cell population doubling - (reply: 1)
  733. Seeding HeLa cells into 96 well plate for transfection - (reply: 2)
  734. Cell line licence - (reply: 1)
  735. adenoviral cre or lentiviral cre for mouse primary cells? - (reply: 2)
  736. temperature tolerance of animal cells - (reply: 3)
  737. only one plasmid expressed highly after co-tranfection into cells - (reply: 1)
  738. pERK and pAKT in MDA-MB-231 cells by western blot! - (reply: 1)
  739. Human insulin for cell culture? - (reply: 2)
  740. violet laser (405nm) and cell cycle staining with DAPI - (reply: 1)
  741. Competent cells question: JM101 vs DH5alpha - (reply: 2)
  742. Trouble producing stable cell lines - (reply: 2)
  743. Infecting cells with virus - (reply: 1)
  744. Requesting basic protocols on cell sorting - (reply: 1)
  745. lysing mammalian cells in sds - (reply: 3)
  746. Is it possible to extract RNA from Cells frozen in 10% DMSO ? - (reply: 2)
  747. Is it possible to assay the carbohydrate profile of a cell? - (reply: 2)
  748. changing cell culture media - (reply: 1)
  749. Preparing Palmitate:BSA solution for treatment of cells? - (reply: 7)
  750. trypsinization of adherent cells - (reply: 9)
  751. Mitochondria pelleted together with nuclei from 293 Cells using Sucrose gradient - (reply: 3)
  752. Aberrant (apparently) nuclear immunostaining for VEGF in Glial cells - (reply: 2)
  753. How to lysis cells for an RNA extraction - (reply: 1)
  754. Problem with THP-1 cell line - (reply: 3)
  755. normalization to protein content or cell number - (reply: 2)
  756. detection of phosphorylation from cell pellet - (reply: 2)
  757. are THP-1 Cells(Monocytes) or Macrophages resistant to Mycoplasma infection or - (reply: 4)
  758. Recommended competent cell for transformation of large plasmids? - (reply: 4)
  759. Cell culture shared with yeast? - (reply: 2)
  760. E. coli cells refuse to grow! - (reply: 2)
  761. how to fraction stem cell at different cell cycle stage - (reply: 2)
  762. Making Nuclei From Mouse Cells - (reply: 1)
  763. Different cell lines different protein stability - (reply: 1)
  764. Neutrophil cell lines and plasma cell lines of choice - (reply: 1)
  765. Immunofluorescence Staining of Cells - (reply: 1)
  766. Nucleus fills the whole cell - (reply: 3)
  767. Generating stable cell line with linear DNA - (reply: 3)
  768. isolating cells from Cockroach - (reply: 2)
  769. Question about suspension cells - (reply: 7)
  770. Calu-3 cell line do not grow properly - (reply: 1)
  771. Detach cells and stop stimulation simultaneously- How? - (reply: 4)
  772. Counting and plating cells - (reply: 3)
  773. cells die 4-6 days after thawing - (reply: 8)
  774. Cell Envelope Proteins Smeared - (reply: 1)
  775. Calculating the amount of the desired cells in the medium ? - (reply: 3)
  776. long term storage of cells at -80 - (reply: 1)
  777. Making BL21 competent cells - (reply: 2)
  778. cell plating density - (reply: 2)
  779. What kind of cell culture contamination is it? - (reply: 8)
  780. Problem with preparing unicellular suspension from MCF7 cells - (reply: 3)
  781. xenograft cells harvested from spleen of SCID/NOD mice - (reply: 1)
  782. to treat cells with inhibitor - (reply: 2)
  783. lysis buffer not lysing adherent cells? - (reply: 8)
  784. Culturing cells on a slide - (reply: 5)
  785. Culturing cells on a slide - (reply: 3)
  786. protocol for immunostaining primery cells - (reply: 5)
  787. How to wipe out fragment of dead cells from the suspension cells? - (reply: 1)
  788. How to Dilute Cell Lysate for Luciferase Assay - (reply: 2)
  789. What's the function of cholera toxin in cell culture medium? eg. MCF10A cell - (reply: 2)
  790. Could the use of different FBS affact my cells - (reply: 5)
  791. Analysis of high throughput screen for cell surface markers - (reply: 1)
  792. Lymphoblastoid cell culture - (reply: 9)
  793. SF9 Cells lose exponential growth, lack stationary phase - (reply: 3)
  794. Don't know why my cells are unhealthy - (reply: 2)
  795. Hybridoma cells not secreting antibody - (reply: 3)
  796. Determining gene of interest from Cell culture lines - (reply: 2)
  797. Three clones of a cell line? - (reply: 3)
  798. Freezing cells in DMSO straight into minus 80? - (reply: 4)
  799. MCF7 cells vitality - (reply: 3)
  800. Ultrasonic bath for cell lysis - (reply: 3)
  801. any methods to evaluate the attaching ability of suspension cells? - (reply: 6)
  802. Vascular Smooth Muscle cell Marker - (reply: 3)
  803. Foam in Cell Culture - (reply: 1)
  804. Where can I find more info on how certain ionic solutions can affect cell biolog - (reply: 4)
  805. SURE cells - (reply: 1)
  806. DT40 Cell Synchronisation - (reply: 2)
  807. Bio-Rad Cell counter - (reply: 3)
  808. SKBR-3 cells dying - (reply: 3)
  809. New cell culture user with no teacher or trainer - (reply: 5)
  810. Help with some problems with Culturing Epithelial Cells - (reply: 5)
  811. NIH3T3 cells are suddenly dying a few days after thawing and expanding - (reply: 1)
  812. Stable cells survive with antibiotics but no exogenous expression detected with - (reply: 2)
  813. What are these large multinucleated cells??? - (reply: 1)
  814. Searching for a live cell staining method - (reply: 1)
  815. WT BRAF/NRAS mouse Melanoma cells - (reply: 2)
  816. HEK 293 cells Detachement - (reply: 8)
  817. Cell culture in candle jar - (reply: 2)
  818. centrifugation speed/cell prep for flow - (reply: 4)
  819. Huge background with HT22 cells phophoERK antibody - (reply: 3)
  820. Huge background with HT22 cells phophoERK antibody - (reply: 1)
  821. Competency cells analysis - (reply: 1)
  822. How many cells needed for co-IP and mass spec - (reply: 4)
  823. Performing MTT assay in suspension cells (new in the subject) - (reply: 7)
  824. cells die after transfection - (reply: 1)
  825. Seeding cells on small metal screws - (reply: 1)
  826. Cell lysate turbidity upon long storage - (reply: 3)
  827. Host cells for transformation - (reply: 3)
  828. Splitting or feeding cells for the weekend - (reply: 1)
  829. Black moving particles in the cell Culture - (reply: 2)
  830. cell culture - (reply: 1)
  831. cells shrink, detach from each other when changing media - (reply: 3)
  832. Newbie help: Seeding cells to 6-well plates - (reply: 2)
  833. 3t3 cell tured black - (reply: 9)
  834. calculating the number of cells to freeze down - (reply: 2)
  835. Fixed cells, sticky cells? - (reply: 2)
  836. What is cotransfection in molecular cell biology? - (reply: 1)
  837. serum deprivation of cell lines - (reply: 1)
  838. serum deprivation of cell lines - (reply: 1)
  839. What to do Post-transfection of cells with siRNA? - (reply: 2)
  840. Problem with HEK stable cell line - (reply: 1)
  841. HEK 293 cell culture - (reply: 3)
  842. HepG2 cells resistant to Trypsinization - Please help!! - (reply: 8)
  843. difficulty in passaging HK-2 cells - (reply: 5)
  844. difficulty in passaging HK-2 cells - (reply: 2)
  845. qPCR in primary cells - housekeeping CT-values sample-specific bad - (reply: 1)
  846. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
  847. More cells in an antibiotic plate than in a plate without antibiotic???? - (reply: 2)
  848. what are these cells?? - (reply: 1)
  849. How to choose the right plasmids for certain types of cell? - (reply: 1)
  850. Isolation of rat endothelial cells - (reply: 2)
  851. protocol for making e.coli competent cells under low temperature - (reply: 1)
  852. Looking for cell surface markers. - (reply: 3)
  853. common nuclear marker for T cells - (reply: 1)
  854. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
  855. Any reliable markers for mouse memoery T cells? - (reply: 1)
  856. Do mouse endothelial cells express the alphaVbeta3 integrin? - (reply: 1)
  857. Cell Culture debris or contamination? - (reply: 4)
  858. primary cell isolation from CNS, how to remove myeline debris - (reply: 1)
  859. Platinum-A cell trasnfection toruble - (reply: 1)
  860. cell culture media refrigeration - (reply: 4)
  861. abnormal epithelial cells in mouse mammary gland - (reply: 1)
  862. cell DNA content of cells grown on different scaffolds - (reply: 2)
  863. Cancer cell starvation - (reply: 1)
  864. Why are HEK-293 cells difficult to perform clonal selction? - (reply: 4)
  865. Housekeeping gene for COS-1 cells - (reply: 1)
  866. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
  867. Monocytes die after cell scraping - (reply: 2)
  868. The water pan got dry in the TC incubator. Will my cells be OK? - (reply: 1)
  869. Cell fractionation - (reply: 2)
  870. Media formulations for MDA-MB-231, BT-474 cells and Caco-2 cells - (reply: 1)
  871. Proper storage of isolated white blood cell prior to RNA isolation - (reply: 3)
  872. Dropped cells into liquid nitrogen tank - (reply: 3)
  873. 5-FOA media not letting ANY cells grow... help! - (reply: 1)
  874. Small intestinal cell line for transport studies - (reply: 4)
  875. help needed in determining volume of cells to be seeded - (reply: 6)
  876. Dilution, Cell concentration & DNA content - (reply: 3)
  877. competent cells - (reply: 2)
  878. Cell lines & gene of interest - (reply: 1)
  879. Software for selection genes related to concrete cell processes - (reply: 1)
  880. Immunocytochemistry with HEK293 cells - (reply: 1)
  881. How to determine apoptotic cell types in-vivo? - (reply: 1)
  882. Isolating Genomic DNA from a small colony of cultured human cells - (reply: 1)
  883. data analysis for single cell size and distribution - (reply: 1)
  884. determining optimal cell count for MTT - (reply: 2)
  885. Extracting DNA from FACS sorted cells - (reply: 3)
  886. Storing frozen cells in -80C instead of liquid nitrogen - (reply: 7)
  887. phosphate + freeze thaw vs RIPA buffer for cell lysis - (reply: 2)
  888. DG44 CHO cells and Methotrexate - (reply: 1)
  889. extract plasmids from transfected eukaryotic cells - (reply: 6)
  890. Staining Cells in Suspension - (reply: 4)
  891. cell culture confusion - (reply: 1)
  892. a gene expressed in 293 cell lines but not Hela cells - (reply: 1)
  893. chambered cell culture-slide for confocal microscopy - (reply: 3)
  894. Nicotiana tabacum (BY-2) Suspension cells contaminated! Help! - (reply: 1)
  895. Dead or Alive... -_- (MCF-7 cells) - (reply: 7)
  896. how to wash cells after fixation for a live binding study - (reply: 1)
  897. Evenly distribute 100ul of cells in a 48/96 well - (reply: 2)
  898. Cell lysis before shearing - (reply: 5)
  899. really low puromycin concentration for selection of cells - (reply: 6)
  900. Single stain control for 293 cell line - (reply: 4)
  901. loading dendritic cell with antigen - (reply: 1)
  902. Cell Lysis and Western Blotting - (reply: 1)
  903. problem with embryo carcinoma cell line , p19 , - (reply: 1)
  904. In vitro induction of apoptosis in tumor cells by inactivated NDV and IAV - (reply: 1)
  905. problem with embryo carcinoma cell line , p19 , - (reply: 1)
  906. Recombinant protein from cell lysate - (reply: 4)
  907. How to test if a compound is entering cells? - (reply: 3)
  908. help with cell seeding - (reply: 2)
  909. SURE or Stbl competent cells for retroviral vector? - (reply: 2)
  910. RNA isolation from frozen cells - (reply: 2)
  911. How do you coat coverslips before seeding cells? - (reply: 3)
  912. Buy.Ask for buying some cell lines and antibodies - (reply: 3)
  913. competentce cell, cloning - (reply: 2)
  914. Flp-recombinase target site integration in 293 T-REx cells - (reply: 1)
  915. Storage of transfected PC12 cells - (reply: 1)
  916. cell adhension - (reply: 1)
  917. Using Methanol For Cell Lysis - (reply: 1)
  918. Adjusting pH of cell culture medium for ELISA - (reply: 1)
  919. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
  920. Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
  921. Contamination of primary cells with fibroblast. - (reply: 1)
  922. Help With Lysing HEK293 & Fibroblast Cells - (reply: 1)
  923. problem with siRNA transfection of HEK cells - (reply: 5)
  924. Splitting A549 cells - (reply: 9)
  925. NMDA Transfection in 293 Cells - (reply: 1)
  926. freezing adherant cells - (reply: 2)
  927. freezing adherant cells - (reply: 2)
  928. Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
  929. How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
  930. cells stably expressing T7 RNA polymerase - (reply: 1)
  931. Cell Fixation - Experience with cell fixation? (reply: 5)
  932. picking up colonies and growing in the broth after transformaion - cloning with competent cells (reply: 1)
  933. Is pCL-Eco suitable for packaging viruses that used to infect human cells? - (reply: 1)
  934. Cause of Death for Cell Culture? - (reply: 5)
  935. creat a SY5Y stable cell line - (reply: 1)
  936. selecting drug-resistant colon cancer cells - (reply: 2)
  937. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
  938. Helper T cell skewing - (reply: 3)
  939. Optimum serum starvation period for NIH, CHO, COS7 cells - (reply: 4)
  940. Help me brainstorm an explanation for this cell culture contamination - Cell Culture, contamination, NIH 3t3 (reply: 7)
  941. competent cells stock - (reply: 3)
  942. intracellular cytokine staining - re-stimulation of activated T cells (reply: 1)
  943. DH5alpha cells - (reply: 4)
  944. pheonix cells and &293A cells - (reply: 1)
  945. could freezing/thawing make mammalian cells competent - could it change phenotype back? (reply: 6)
  946. Protein purification from HEK 293 cells - (reply: 1)
  947. Growing hybridoma cells in medium without serum - (reply: 2)
  948. Cells sensitive after replacing media - what am I doing wrong? - (reply: 4)
  949. Cell drawing tools? - (reply: 6)
  950. How many cells in plant embryos ? - seed biology (reply: 1)
  951. cell count - (reply: 8)
  952. question on making chemical and electrocompetent cells - (reply: 2)
  953. COS7 cells giving me problems - (reply: 1)
  954. Where to get 5T series cells? - (reply: 1)
  955. cell number for colony formation - HEK293, NIH3T3 (reply: 1)
  956. Electroporation/electrotransfection mammalian cells - (reply: 7)
  957. Cell debris here, there, everywhere!? - (reply: 2)
  958. Cell debris here, there, everywhere!? - (reply: 1)
  959. Mini prep of frozen bacteria cells - (reply: 2)
  960. Help! Turned on UV light. Did I kill my cells? - (reply: 2)
  961. growing competent cells - (reply: 1)
  962. Freezing density for NIH 3T3 cells - (reply: 3)
  963. Stable Cell Line generation - (reply: 3)
  964. making more competent cells - (reply: 2)
  965. CellTracker dye in necrotic cells? - (reply: 1)
  966. The case of the punky Sf9 cells - (reply: 2)
  967. sticky perm cells (tween 20)- can I prevent it? - (reply: 2)
  968. Preparation of mammalian cell lysis - Preparation of mammalian cell lysis for Western blotting (reply: 7)
  969. collecting cells HT1080 - (reply: 4)
  970. Expired media - How long after the expiration data is cell culture media good? (reply: 2)
  971. Help: problem with MDCK cells - (reply: 2)
  972. bacterial Cell free culture supernatants - Can any one help me out to prepare a cell free culture supernatant (reply: 3)
  973. Oh my cell culture problems never cease!!! - (reply: 4)
  974. WEHI-3BD cell culturing - Advice on culturing. (reply: 5)
  975. cell homogenization - (reply: 13)
  976. cell count - (reply: 3)
  977. cell lysis - confocal slides (reply: 4)
  978. HeLa cell anatomy question - (reply: 3)
  979. Need cell lines wich express separately TLR 2, 4 and 5 - (reply: 1)
  980. MSC in culture - stop growing? - Mesenchymal stem cells in culture randomly stop expanding (reply: 2)
  981. THP-1 cell line - (reply: 3)
  982. CaMKII alpha promoter and cell tranfection - (reply: 1)
  983. Adaptation of mammalian cells to serum free mode - (reply: 2)
  984. Live cell Imaging medium - (reply: 1)
  985. Transfection of CHO cells with Fc receptor - Problems with expression murine Fcgamma recptor (reply: 1)
  986. MDA-MB-231 cell transfection - (reply: 5)
  987. Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
  988. Break Cell Clumps - prepare single cells for injections (reply: 1)
  989. Stem Cells Sphere - stem cells (reply: 2)
  990. Request for cell lines? - (reply: 1)
  991. Is 2% pen strep too much for cell culture? - will my cells die a painful death? (reply: 3)
  992. MTT problems with Huh7 cell line, plzzzzzzz help - MTT color problem (reply: 5)
  993. Calculating cell doubling time - (reply: 1)
  994. SPONTAEOUS DEATH OF CELLS in culture - (reply: 8)
  995. Infected cells that are dying after puromycin selection - (reply: 1)
  996. contamination issue in cell culture - (reply: 1)
  997. Why do cells need to be washed in PBS before biotinylation? - (reply: 3)
  998. AAD positive means late apoptosis? - About cells that are simultaneously positive for annexin V and AAD (reply: 2)
  999. Purification of recombinant proteins from insect cells - (reply: 2)
  1000. Cell cycle evaluation in cells with more than one nuclei - (reply: 8)
  1001. Help! Transfection newbie with a dumb question - Trying to transfect adherent cells (reply: 1)
  1002. Tissue vs cell line lysate - Improve sample quality? - (reply: 4)
  1003. Growth curve for PC3 cell line - (reply: 1)
  1004. Agrobacterium EHA101 competent cells - (reply: 1)
  1005. THP-1 cell line - (reply: 2)
  1006. Effective DCFDA Protocol for Non-Adherent THP-1 Cells? - (reply: 1)
  1007. Stripping Buffers that won't kill my cells? - Recipe for 0.1 M Tris-Acetate 2.0 M NaCl pH 7.7 (reply: 3)
  1008. best freezing method for electrocompetent cells - (reply: 9)
  1009. cell seeding calculation - (reply: 8)
  1010. Improved Stem Cell Proliferation - Would interested in improving proliferation of adult stem cells? (reply: 5)
  1011. Can one use Magnesium chloride for competent cell prep. - (reply: 1)
  1012. Yeast contamination in cell culture - (reply: 8)
  1013. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
  1014. Remove cells from dish and fix them in slides - (reply: 3)
  1015. Help- lysing cells with triton x-100 - (reply: 1)
  1016. E.coli cells lyse in minimal media - (reply: 3)
  1017. How stable are suspension cells in room temperature? - (reply: 2)
  1018. Help with HELA and HEK293 cell seeding for transfection - (reply: 4)
  1019. Transfection in insect cell lines?? - (reply: 1)
  1020. PCR genomic DNA using cell lysate - (reply: 1)
  1021. HELA Cells Capable of producing whole, infective HPV particles? - (reply: 3)
  1022. Distilled water for Electrocompetent Cell Preparation - (reply: 6)
  1023. Should I fix cells digested from tissues? - (reply: 4)
  1024. STR cell line identification - looking for cell line identification method (reply: 1)
  1025. Establishing Sub-cutaneous Xenograft Cancer Model - How many millions of cells to inject for subcutaneous models (reply: 3)
  1026. Rat Dental Pulp Stem Cell Culture - (reply: 1)
  1027. NK cell analysis using CD16PE and CD56PE - (reply: 2)
  1028. My neuroblastoma are floating!! please help - Both SK-NSH and SH-SY5Y are adherent cells, but are only floating now (reply: 3)
  1029. cell suspencion and medium - (reply: 5)
  1030. One Shot TOP10 Cells - (reply: 6)
  1031. Help on BmN4 cell culture - How to maintain BmN4 cells? (reply: 1)
  1032. Compare ChIP to cell lysate - (reply: 1)
  1033. Compare ChIP to cell lysate - (reply: 1)
  1034. Testing cytoxixity on healthy human cells - (reply: 2)
  1035. what cell line to use - to demonstrate ubiquitination (reply: 1)
  1036. GFP cells intracellular staining - (reply: 1)
  1037. Xenograph - how to maintain cell viability? - (reply: 1)
  1038. Western blot of apoptotic cells - (reply: 2)
  1039. cell sycle and proliferation - (reply: 3)
  1040. help with cell dilution - (reply: 2)
  1041. Difficult Cell Proliferation Experiment - (reply: 2)
  1042. Cell dilution cloning - Cells cloning (reply: 2)
  1043. Which cells to use ? - Chang liver or normal breast cells ? (reply: 1)
  1044. HTC116 cells detaching !!!! - (reply: 2)
  1045. Revival of kasumi-1 cells - (reply: 3)
  1046. Growth conditions for E. coli DB3.1 cells - (reply: 1)
  1047. weird & curious problem with CHO cell culture - (reply: 3)
  1048. HaCat do not grow after using cell scraper - (reply: 5)
  1049. MTT assay problems (Cells just come off after incubation) - Cells just come off after incubation in 37 degree (reply: 1)
  1050. Cell Lysates from a Western Blog - (reply: 2)
  1051. Loss of YFP signal in transduced T cells - (reply: 7)
  1052. Overexpression of mouse constructs in human cell lines - some questions (reply: 6)
  1053. Freezing cell pellets and minimizing lysis - (reply: 2)
  1054. Additional peaks in cell cycle assay - Can 37°C incubation cause clumping of fixed cells? (reply: 6)
  1055. Total Cell protein - (reply: 1)
  1056. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
  1057. cell culture media - glutamine! - (reply: 2)
  1058. How many cells do you have in a confluent T 75 flask? - I have 1/2 million (reply: 2)
  1059. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
  1060. Premature cell lysis - (reply: 2)
  1061. urgent!!! weird Growth in cell line - (reply: 8)
  1062. Shipping of blood cells over long distance - (reply: 1)
  1063. refrozen cell pellet - (reply: 1)
  1064. Drug vehicle giving toxicity in cell culture - With increse in vehicle..... toxicity decrese (reply: 4)
  1065. contamination in cell line...... - (reply: 4)
  1066. Neuronal Cell Culture Woes - Not even making it out of the gate! (reply: 7)
  1067. Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
  1068. MTT with cells growhing in suspension, not adherent - (reply: 3)
  1069. Cell ELISA - No differences between cells expressing and not, the receptor (reply: 2)
  1070. growth curve for attached cells - (reply: 1)
  1071. cells dont attach hard enough to 96 well - (reply: 3)
  1072. CTLL-2 culture - preventing cell clumping - (reply: 2)
  1073. Yet another contamination question - SF9 cells (reply: 11)
  1074. Problems with transfection in human cells - (reply: 3)
  1075. HepG2 cells culture conditions - (reply: 2)
  1076. Should I use fully supplymented medium or low serum medium when treat INS1 cells - (reply: 4)
  1077. T cell proliferation problem - (reply: 3)
  1078. How to Handle Cells Shipped Internationally in Dry Ice? - (reply: 2)
  1079. Differences between cells in different species - (reply: 5)
  1080. Will pervious PBS concentration in medium have a long lasting effect on cell gro - (reply: 2)
  1081. isotype control for GFP expressing cells? - (reply: 2)
  1082. Trouble with growth of transformed cells - (reply: 4)
  1083. nuclear extraction of blood cells - (reply: 2)
  1084. Problems about detection of apoptotic cells using Annexin V/PI staining - (reply: 2)
  1085. ELISA with undiluted cell lysate samples - (reply: 1)
  1086. How can you expand cells if you have one dish of them? - (reply: 1)
  1087. Androgen independent LNCaP cells - (reply: 2)
  1088. What is the proper way to infect cell culture with virus? - (reply: 5)
  1089. Micro-RNA profiling for differentiating cells - (reply: 1)
  1090. Growing insect cells with other cell lines - (reply: 2)
  1091. Candida albicans cells lysis - method (reply: 1)
  1092. Cell cycle analysis after viral transduction - Shift of peak (reply: 1)
  1093. Advise for Whole Cell Stain - hESC-differentiated Trophoblast (reply: 1)
  1094. 293 T cells - (reply: 3)
  1095. Mouse Bone Marrow Isolation - How to increase yield of BM-derived cells? (reply: 1)
  1096. BL-21 Gold (DE3) cells and further strains - (reply: 2)
  1097. Cell Cycle Analysis flow cytometry - How to measure desynchronisation rate of cells (reply: 1)
  1098. Simple & straightforward question - Buffering agent for CHO cell medium (reply: 1)
  1099. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
  1100. Fixing cells before staining of surface markers - (reply: 1)
  1101. Black dots in cell culture - (reply: 7)
  1102. cell counting - (reply: 5)
  1103. Optimization for cell viability assay (MTT assay) - (reply: 1)
  1104. Anyone thought about reprogram fibroblast to liver cells? - (reply: 2)
  1105. cell confluence in cell cultures - myoblast c2c12 in particular (reply: 2)
  1106. cell line adherence - cells adhering to flask very tightly after transfectiono (reply: 1)
  1107. Cell labelling - How can I label 2 cell populations? (no transgenes) (reply: 6)
  1108. How long does it take for cells to reattach? - From balls to pancakes. (reply: 3)
  1109. Dot plot cell cycle analysis-strange dots - (reply: 4)
  1110. TERT transformation of fibroblast cells - Immortilising fibroblast cells. Which companies available? (reply: 1)
  1111. Apoptosis vs Viable Cells vs Necrosis (Annexin/PI Staining) - Which quadrants to sum up? (reply: 4)
  1112. help Splashed Raji cells on skin/face/hand - (reply: 2)
  1113. Help! ES Lenti infection efficiency=nightmare - trouble getting over 40 % cells infected (reply: 8)
  1114. Staining corneal endothelial cells for mechanical damage with methylen blue - (reply: 1)
  1115. CO2 level for cell culture - (reply: 6)
  1116. suspension cells for confocal - (reply: 1)
  1117. RNAi is somatic and germline cells - (reply: 3)
  1118. Luciferase and cell responsiveness - (reply: 3)
  1119. adult cell culture - (reply: 1)
  1120. protein overexpression in HeLa cells - (reply: 1)
  1121. Insulin supplements to cell culture media - (reply: 1)
  1122. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
  1123. RNA/cell accumulation of difficult-to-obtain tissue - (reply: 1)
  1124. Bacteria to Cell Culture - How many bacteria should I add to cell culture (reply: 2)
  1125. topic and cell culture - (reply: 2)
  1126. Running a western with cell media in gels? - (reply: 11)
  1127. Starting cells for making one's own competent cells - (reply: 7)
  1128. cell loss during antibody staining for flow cytometry - FACS staining protocol (reply: 5)
  1129. Persistent stable cell line (Bos23) Contamination... - (reply: 2)
  1130. Hematopoietic cells separation - How to get high purity and viability hematopoietic cell populations? (reply: 2)
  1131. Transfection of large plasmid - How to get a 10kb plasmid into HEK293 cells (reply: 3)
  1132. min6 cells immunofourescence staining - staining and culture problem (reply: 4)
  1133. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
  1134. why the cells transformed with linearized plasmid cannot grow? - (reply: 3)
  1135. Rabbit cells to grow bacteria in before immunization of rabbit for antibody prod - (reply: 2)
  1136. rat ES cells - (reply: 2)
  1137. Neuronal Cell Culture - Media Changes - How to change media half and half (reply: 4)
  1138. establishment of primary cell line from biopsy - (reply: 2)
  1139. Cell culture fasting - (reply: 1)
  1140. mutated HeLa cells? - from cycloheximide? (reply: 1)
  1141. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
  1142. qPCR for transformed cells - (reply: 1)
  1143. solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 1)
  1144. A solution to a weird problem - pH - Adjusting pH in cell culture media (reply: 6)
  1145. descrepancy between total cell lysate and cell fractionation - (reply: 1)
  1146. Disruption of cells using mild detergent - (reply: 2)
  1147. Eruption of mammalian cells using detergent - (reply: 1)
  1148. lenti virus transfected cell dies... - (reply: 4)
  1149. Extremely Weird Issue - CHO Cell Culture (reply: 2)
  1150. Cell Die.. Need help - (reply: 1)
  1151. Cell scraping ruining my life - (reply: 6)
  1152. Death of cells in the control. - (reply: 1)
  1153. cell culture clarification - I need help please (reply: 2)
  1154. Serum concentration in treatment of cells for MTT assay - (reply: 1)
  1155. Minimum number of cells needed for cytometry? - (reply: 3)
  1156. Beta-Lactam in CHO Cells - (reply: 1)
  1157. problems with 293T cells after medium removal!!! - (reply: 6)
  1158. B cell subset for hybridoma generation - Which B cell subset is used to make hybridomas (reply: 2)
  1159. Propagating ccdB survival cells - (reply: 6)
  1160. Western difficulty in primary neuron cells. - (reply: 1)
  1161. DOubt - Competent cell (reply: 2)
  1162. Differentiation of CD11b cells into macrophages.Help please - (reply: 1)
  1163. Primary keratinocyte isolation- cell loss - (reply: 1)
  1164. cells attach but don't flatten out - MDA-231 (reply: 8)
  1165. Problem Generating Stable HeLa Cells - (reply: 1)
  1166. Retroviral transduction of T cells - (reply: 4)
  1167. Looking for "biocell" from the forum archives. - Cell culture contamination problems.... (reply: 1)
  1168. p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells - p21, p27, Phos-Akt & Phos-Erk 1/2 Expression in KASUMI 1 Cells (reply: 9)
  1169. Multiple nuclei?? - cell morphology - FaDu (reply: 8)
  1170. Viral RNA is relatively stable than mRNA from cells? - (reply: 1)
  1171. how to compensate the cell death during transfection by electroporation - (reply: 2)
  1172. MCF-7 CULTURING - (reply: 9)
  1173. H-ras transformed RK3E/tv-a cells - (reply: 1)
  1174. HEK001 Cell Line and Papillomavirus - Does HEK001 cell line produce a functional papillomavirus (reply: 4)
  1175. 2 questions: myeloma cell lines and mice MABs - (reply: 5)
  1176. I'm getting fried-egg looking cells... What should I do?? - (reply: 8)
  1177. Empty bubbles in the 3T3-L1 cells - (reply: 3)
  1178. Exposing Cell Lines to Drugs Metabolized by Liver Microsomes - (reply: 1)
  1179. UV safety in cell culture hood - (reply: 3)
  1180. Where can I find info on the physical appearance of TB cell wall structures? - I am an artist wanting to depict the cell wall with absolute accuracy. (reply: 7)
  1181. intracelluar staining without killing cells - (reply: 3)
  1182. Info about A7r5 cell culture - (reply: 1)
  1183. Continuation? Kombucha Tea and MCF-7 - (reply: 5)
  1184. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  1185. Cell lysates - Protein precipitation? - (reply: 3)
  1186. Glucose in epithelial cell - (reply: 1)
  1187. cell lysate for pulldown - (reply: 3)
  1188. cell culture media - (reply: 3)
  1189. Competent cell storage for long term use - (reply: 2)
  1190. My RIPA cell lysis buffer - Western blot (reply: 3)
  1191. Cell Uptake Study - (reply: 1)
  1192. what will happen if my cell culture have exposed to unfiltered air for a few sec - (reply: 2)
  1193. aspects of RAW264.7 cells - (reply: 5)
  1194. anti his antibody for western blotting - high background in cell lysate western blotting (reply: 3)
  1195. Conditioned Medium - Primary skeletal muscle derived cells conditioned medium (reply: 3)
  1196. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
  1197. Hoechst Stain does it stain dead cells - (reply: 3)
  1198. Help for cell preparation for FACS... - (reply: 1)
  1199. Cell Lysis Buffer - (reply: 6)
  1200. HeLa cells and HPV - (reply: 2)
  1201. Resolving cell growth problems using growth additives - way to resolve urgent cell growth problems (reply: 3)
  1202. Low transformation efficiency - Rosetta-gami™ 2(DE3)pLysS Competent Cells (reply: 8)
  1203. problem with MACS cell isolation - (reply: 5)
  1204. cell culture split ratios. - (reply: 2)
  1205. MDA-MB-231 cell culture issue - after thawing first passage is fine, next goes bad (reply: 7)
  1206. blood red cells - (reply: 1)
  1207. Attention-seeking cell cultures - reduce subculture ratio? - QM7 cells do the darndest things (reply: 2)
  1208. NFKb signalling in cell line ??? - (reply: 2)
  1209. kit fpr cell membrane preparation? - (reply: 2)
  1210. Retrovirus Production - I am not getting any virus from my packaging cells! (reply: 4)
  1211. How to find NFKb signalling is active in a cell line??? - (reply: 2)
  1212. Fixing YFP cells - (reply: 2)
  1213. MTT Assay: cell proliferation or cell viability??? - (reply: 4)
  1214. Rescue RAW cell culture - RAW cells past confluent point... can they be rescued? (reply: 4)
  1215. cell cycle arrest by colcemid - (reply: 2)
  1216. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
  1217. CD19 Expression at various stages of plasma cell differentation - (reply: 1)
  1218. PROLIFERATION ASSAY IN MICROTITER PLATES WITHOUT A SEMIAUTOMATIC CELL HARVESTER - (reply: 1)
  1219. THP cells? what does THP stands for?????????? - (reply: 1)
  1220. Immunioprecipitation from Jurkat cells?? - (reply: 1)
  1221. Weird effect of siRNA on protein in cells - (reply: 3)
  1222. why I cannot test expression in transduced cells - Can test expression in transfected cells, but not in infected cells (reply: 3)
  1223. Apoptosis induction - for vero and mcf-7 cells (reply: 5)
  1224. Need pH 6 Cell culture media - (reply: 1)
  1225. Are my cells undergoing epigenetic changes? - (reply: 2)
  1226. establishment of stable cell line using hek293t - (reply: 2)
  1227. Cell pellet frozen -80 then -20 - (reply: 4)
  1228. total cell protein vs membrane protein - (reply: 4)
  1229. tranfection of shRNA into mammalian cells is causing the cells to die - (reply: 4)
  1230. transfection efficiency - i am looking for a cell line that its transfection efficiency is high (reply: 4)
  1231. Fixing the cells for FACS - Paraformaldehyde or Formaldehyde (reply: 2)
  1232. Problem with control samples in cell IC - (reply: 3)
  1233. Recommendation for cell cycle analysis software needed - (reply: 1)
  1234. Tips on fermenting DH5alpha and MG1655 - e.coli cells never leave lag phase during fermentation (reply: 1)
  1235. Cell culture contamination question - what the hell are these things?? - (reply: 3)
  1236. 'Stringy' contamination of cells? - Weird stringy thing in cell culture flasks (reply: 3)
  1237. Modified PBS buffer for red blood cells washing - [urgent, need opinions] (reply: 2)
  1238. Immunofluorescence using dead cells? - (reply: 1)
  1239. floating THP-1 cells - medium change advice - (reply: 2)
  1240. my cells love infection, any thoughts on antibiotics? - (reply: 3)
  1241. Culture Media for stem cells - (reply: 1)
  1242. Trypsinization or Scraping for adherent cells in ChIP assay? - (reply: 8)
  1243. Anyone heard of cells spontaneously fluorescing red?? - (reply: 4)
  1244. Freezing cells - How long can I keep cells at -80C? (reply: 6)
  1245. Help regarding cell line - (reply: 1)
  1246. organizing cell lines - (reply: 1)
  1247. Cell plating density - (reply: 3)
  1248. Murine antibodies - A panel of antibodies for murine blood cells (reply: 2)
  1249. transfecting cells with mature miRNA or precursor miRNAs? - (reply: 5)
  1250. MDA-MB-231 cells question - (reply: 3)
  1251. thawed cells wont adhere - pc3 and LNcaP (reply: 2)
  1252. DiFi Cell line (colon carcinome) - Where can I get it???? (reply: 1)
  1253. HaCaT cells - (reply: 2)
  1254. Why does my media turn alkaline not acidic? - MCF-7 in EMEM (reply: 4)
  1255. lncap and MCF-7become ethanol sensitive? - are lncap cells and MCF-7 cells ethanol-sensitive? (reply: 2)
  1256. Dying endometrial stromal cells - (reply: 1)
  1257. Help me - Are my cells infected with something or could this be unsusual morphol - (reply: 5)
  1258. how to dissociate cells for cell migration assay - (reply: 3)
  1259. soft agar assay - cells in the top agar cells adhere to the plates unexpectedly. (reply: 4)
  1260. RNA extraction - how many cells do I need at least?? (reply: 4)
  1261. RNA extraction protocol for small amounts of cells - (reply: 2)
  1262. about estrogen receptor beta negtive cell lines - can anyone give me some suggestion about ER beta negtive cell lines? (reply: 1)
  1263. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
  1264. strange morphology of sp2/0 cells after fusion!!! Please help! - morphology switch from round to triangular!!!! (reply: 2)
  1265. fabrablast primery cell line problem - how to detach cell well (reply: 1)
  1266. what solvent is better compatible for most cell lines? - DMSO vs methanol (reply: 3)
  1267. Cell growth rate & O2 consumption - (reply: 2)
  1268. I did not change the media the day after transfecting 293T cells. Are they going - (reply: 1)
  1269. Cells stick to dish in Embryoid Bodies - (reply: 1)
  1270. Changing the media after transfection of 293T cells - I did not change the media the day after transfecting 293T cells. (reply: 7)
  1271. Non Mouse / Human Cell Line that is easily transfected - (reply: 2)
  1272. how to plate cells precisely - (reply: 1)
  1273. RNA extraction from stained cells - (reply: 2)
  1274. Methods to assess cell viability - (reply: 1)
  1275. suspension cell transient transfection - (reply: 10)
  1276. Adherent cells washing off in 96 well -MTT - (reply: 4)
  1277. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
  1278. plasmids - plasmids for stable cell line construction (reply: 2)
  1279. abnormal nuclear morphology after treating shRNA - confocal, shRNA, nuclear morphology, cell division (reply: 2)
  1280. average total protein content per 293t cell - (reply: 2)
  1281. T cells preparation for FACS - (reply: 1)
  1282. counting cells - (reply: 3)
  1283. Cell OD and spec problems - (reply: 1)
  1284. cell confluency & effect on cells...?? - (reply: 4)
  1285. Fixing cells for flow cytometry - alternative to 1% paraformaldehyde (reply: 3)
  1286. Washing Red Blood Cells - Tips on washing red blood cells (reply: 8)
  1287. MEF Cell marker - (reply: 1)
  1288. (CELL CULTURE) Serious advice needed regarding my career..? - (reply: 2)
  1289. Change of colour in cell culture media...? - (reply: 2)
  1290. cell count - how to calculate (reply: 2)
  1291. generating cell line knockouts - (reply: 3)
  1292. Cancer Stem Cells?? - (reply: 10)
  1293. Some kind of contamination? - mouse transduced cell line (reply: 7)
  1294. Cell stimulation of transcription - (reply: 5)
  1295. Mouse blood density/osmolarity of blood cells for separation - (reply: 5)
  1296. suitable cell lysis buffer - (reply: 1)
  1297. Toluidine blue staining for cell culture - (reply: 1)
  1298. cell culture - 3D cell culture (reply: 1)
  1299. Aspergillus in cell culture room - HELP!!! how hardcore to decontami - Cell culture, contamination (reply: 6)
  1300. Transforming rosetta+chaperone cells with topo plasmid - (reply: 5)
  1301. MEF culture contamination? - Contaminated cell culture? (reply: 2)
  1302. very simple question about hemocytometer cell counting - (reply: 1)
  1303. nucleic acid extraction from silenced cells - (reply: 6)
  1304. direct lysis of cell pellet - How to get rid of DNA? (reply: 3)
  1305. In Vitro Drug Exposure Cytotoxicity Testing and Cell Confluency - (reply: 8)
  1306. Dendritic cells - Protocol of stimulation of dendritic cells (reply: 3)
  1307. giant cells in HEK293 cell line - (reply: 10)
  1308. Plant cell transfection - (reply: 1)
  1309. stable cell line - selection after retroviral infection (reply: 1)
  1310. Storing cell lines long-term at -70C - (reply: 4)
  1311. Commercially available primary mouse endothelial cell line? - (reply: 2)
  1312. Frozen cells in -80 - (reply: 1)
  1313. Plant or Fungal Cell Dissociation Technique? - (reply: 3)
  1314. how can i bring cells onto a slide? - ...for microscopical observation! (reply: 7)
  1315. cell density to be seeded? - cytotoxicity studies (reply: 1)
  1316. Lysis of the cells - (reply: 1)
  1317. Cells do not lyse in SDS buffer - (reply: 1)
  1318. Storage of Fixed Cells for Immunofluorescence staining - (reply: 1)
  1319. lysis buffer for lymphoblast cells - (reply: 1)
  1320. problem with cell cycle analysis - (reply: 3)
  1321. THP-1 Cells differentiation - THP-1 cells differentiated using IFN-gamma/LPS (reply: 7)
  1322. Single cell suspension from solid tumor - (reply: 3)
  1323. cell aggregation? - (reply: 1)
  1324. T-Cell receptor - Is it a single type on a T cell or multiple types? (reply: 1)
  1325. cells weak adhesion - detachment (reply: 4)
  1326. how we count the passages in cell cultures? - (reply: 5)
  1327. resolving cell growth problems - (reply: 2)
  1328. clumping of cells - (reply: 7)
  1329. stability of cell suspension - (reply: 1)
  1330. cell adhesion in serum free condition - (reply: 1)
  1331. effects of cell confluency - (reply: 1)
  1332. Processing cell samples for low abundance proteins - direct lysis with SDS-PAGE loading buffer (reply: 2)
  1333. Good Cell line for Histone 3 and 4 - Positive Control for Basic Staining and WBs (reply: 1)
  1334. polarized cells - (reply: 4)
  1335. PCNA (proliferating cell nuclear antigen) - is it an adequate marker of proliferating cells? (reply: 2)
  1336. cell adhesion assay - using collagen (reply: 1)
  1337. Problem clariflying/filtering insect cell lysate - (reply: 2)
  1338. cell culture urgent!! - (reply: 2)
  1339. Moving S2 cells from one plate to another - (reply: 1)
  1340. Cell culture medium instead of Nucleofector solution?? - (reply: 3)
  1341. Nucleofection of ES cells, medium instead of nucleofector solution? - (reply: 1)
  1342. mononuclear cells medium - I am stuck with the concentration of 2-mercaptoethanol (reply: 1)
  1343. Accidental thawing of frozen cells - (reply: 3)
  1344. how to start with a new cell line ? - how to start with a new cell line ? (reply: 1)
  1345. HaCaT cells die after trypsinization - (reply: 1)
  1346. Transporting some bacteria cells - transport bacteria cells pellet (reply: 2)
  1347. GFP and Luciferase Cell Line - (reply: 2)
  1348. Transfecting primary cells with plasmid - (reply: 1)
  1349. transfecting and selecting EL4 cells - (reply: 1)
  1350. Induce Osteoblast lineage from C2C12 myoblast cells - (reply: 1)
  1351. Lentivirus production with LNCX vector - Do I have to use Packaging cell lines? (reply: 3)
  1352. Issue on mycoplasma detection and treatment in cell culture - (reply: 3)
  1353. Transfection into eukaryotic cells - (reply: 1)
  1354. Fixing cells with PFA - (reply: 1)
  1355. A549 cells not doubling - has anyone been through this with other cell lines? (reply: 4)
  1356. Immunofluorescence in HEK cells issues - (reply: 1)
  1357. cell sorting - losing cells? - (reply: 3)
  1358. What if my expressed protein is toxic for the cells... - (reply: 1)
  1359. competent cells - difference between electroporation and chemical comp. cells (reply: 1)
  1360. Possible contamination of leukemic cell culture - debris, growth inhibition, cell aggregates (reply: 3)
  1361. human cancer cell line splashed into my eyes - (reply: 3)
  1362. Saving tissue and single cell suspension - FACS, single cell suspension (reply: 1)
  1363. BL21(DE3)LysS competent cells - (reply: 1)
  1364. ATP turnover in neuron - ATP hydrolysis rates in neuronal cells (reply: 2)
  1365. Adhering CHO cell culture - cells don't adhere! (reply: 2)
  1366. do e.coli cells get ruptured during high spin - (reply: 1)
  1367. Please Help with COS-1 cells^^ - (reply: 1)
  1368. IPTG Induction and cell lysis issues - pET 21b system (reply: 1)
  1369. cell counting - to get an impression when my cells are in the log phase (reply: 1)
  1370. Whole cell lysis of fibroblasts - (reply: 1)
  1371. Whole cell lysis of fibroblasts - (reply: 1)
  1372. test of cytotoxic effect of compounds - why cells are pretreated with actinomycin C ?? (reply: 1)
  1373. Proliferation Assay - Should cells be in the logarithmic phase ? (reply: 3)
  1374. cell density and growth - (reply: 5)
  1375. Laser capture microdissection - capturing single cells (reply: 2)
  1376. MCF-7 in cytotoxicity assay - (reply: 2)
  1377. when cells have to be synchronized?? - (reply: 2)
  1378. cells in RNAlater - the title say`s it all :) (reply: 4)
  1379. Cytotoxicity assay for adherent cells exposed to particles - (reply: 1)
  1380. Murine Cell Lines - (reply: 2)
  1381. Cell Proliferation: To adhere or not to adhere (before treatment), that is the q - (reply: 2)
  1382. why are some cell lines more popular than others?? - (reply: 4)
  1383. NK cell staining - (reply: 4)
  1384. cell culture media - with or w/o L-glutamine? (reply: 2)
  1385. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
  1386. pro-apoptotic effect of compounds on cancer cell lines - which cell line to test the non-toxic effect? (reply: 3)
  1387. Question in Generating double stable cell line - (reply: 3)
  1388. activity of sex-chromosome in somatic cells - (reply: 5)
  1389. CaCl2 for making competent cells - (reply: 3)
  1390. Calcium Phosphate Transfection for CV1 CELLS - (reply: 2)
  1391. ICC Cell Adherence Issue - (reply: 1)
  1392. gentamycin resistance in human cencer cell culture - please suggest the alternative antibiotic (reply: 2)
  1393. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
  1394. How to normalize my samples before I transfect my cells - (reply: 6)
  1395. Isolation of intestinal epithelial cells - Isolation of intestinal epithelial cells (reply: 2)
  1396. Number of proteins expressed in cell - (reply: 2)
  1397. Do HEK 293 cells contain functional E1A/E1B? - (reply: 2)
  1398. Sodium Pyruvate - What is it's use in (NIH 3T3) cell culture? (reply: 4)
  1399. Calcium phosphate method vs lipofectamine - troublemaker neuroblastoma cells (reply: 7)
  1400. Which cell lines are best for transient transgene expression? - (reply: 3)
  1401. Alzheimer's cell model - (reply: 7)
  1402. Partial cell subculture? - (reply: 2)
  1403. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
  1404. MCF-7 passage number - (reply: 1)
  1405. Whole Kidney primary cell culture - (reply: 1)
  1406. dh5 alpha competent cells? - (reply: 2)
  1407. which is the best lysis buffer for whole cell extracts? - (reply: 1)
  1408. Please help me to understand- cell counting - (reply: 1)
  1409. Preparing competent cells under laminar flow? - or on the bench sufficient? (reply: 5)
  1410. Cell count - Please help - (reply: 11)
  1411. What is DME medium for Hybridoma cell culture - is DME medium and DMEM medium are the same for hybridoma culture?? (reply: 1)
  1412. Cell Count - (reply: 1)
  1413. Generating stable cell line - (reply: 2)
  1414. Harvesting cells off of collagen gels - (reply: 1)
  1415. cells can't be lysed after crosslinking - (reply: 4)
  1416. How many cells to seed? - simple question :) (reply: 5)
  1417. Is there a problem by adding HCL to the cell culture medium in order to manipula - (reply: 1)
  1418. mouse macrophage cell line - (reply: 5)
  1419. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
  1420. cell line information - require (reply: 1)
  1421. Urgent problem - Media change = cells die in 48h (reply: 3)
  1422. recombineering for ES cell targeting - retrieval problems (reply: 1)
  1423. Rat monocyte cell line - (reply: 1)
  1424. How often do you thaw new cells? - passage number or time? (reply: 7)
  1425. Does high speed centrifugation destroy viable cells? - (reply: 2)
  1426. Cell culture - (reply: 7)
  1427. Problems when plating neuroblastoma cells - (reply: 3)
  1428. Is pFLAG expression toxic? - FLAG seems to kill my HeLa cells. This can't be right (reply: 1)
  1429. Cell Doubling Time - (reply: 3)
  1430. Dimethyl Sulfate (DMS) as carrier in cell cultures - (reply: 1)
  1431. Amount of fixation buffer - is it dependent on the number of cells? (reply: 2)
  1432. synchronized cells or non-synchronized cells - (reply: 3)
  1433. pre-prepararation of competent cells - (reply: 3)
  1434. ROS accumulation - ROS reactive oxygen species accumulation in cells (reply: 3)
  1435. Bacterial invasion assay with human cells - (reply: 2)
  1436. Measuring gene expression across cell lines - (reply: 2)
  1437. How to make an isotonic solution for h9c2 cells - (reply: 2)
  1438. migration < invasion??? - More cells migrating through matrigel than through control inserts (reply: 1)
  1439. Zeocin of G418. Which is the better selection agent for CHO cells? - (reply: 1)
  1440. Immunofluorescence with transfected cells - (reply: 3)
  1441. Cloning Problem (cells grow but no plasmid isolated) - (reply: 13)
  1442. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
  1443. Contamination in cell cultures - (reply: 9)
  1444. DMSO killing more cells at lower concentrations - (reply: 1)
  1445. frozen competent cells - how to prepare competent cells from frozen bacteria? (reply: 3)
  1446. Transient Transfection - Hol long is plasmid on transfected cells? (reply: 4)
  1447. lysing cells on ice - (reply: 2)
  1448. Fibroblast cell culture - (reply: 1)
  1449. Cardiac stem cells - (reply: 3)
  1450. How to visualize tumor cells in mice with out sacrificing the animals - (reply: 3)
  1451. cell count and dilution - (reply: 1)
  1452. z competent cells - (reply: 2)
  1453. immunofluorescence staining of sperm cells - (reply: 2)
  1454. Cell Culture procedure(manual, protocole, what ever ;) - (reply: 1)
  1455. colony formation assay - how many cells to seed (reply: 2)
  1456. Primaria/gelatin plates and gravity-defying fixed cells - (reply: 2)
  1457. Contaminations in cells cultured in suspension - (reply: 2)
  1458. suspension cell transient transfection - (reply: 2)
  1459. What is this ? - Cell culture contaminant (reply: 2)
  1460. cell cryopreservation after siRNA transfection - (reply: 1)
  1461. Cell Digestion Protocol - (reply: 1)
  1462. spin hek cell lysate after adding laemmeli buffer? - (reply: 1)
  1463. cell growth - (reply: 4)
  1464. primary cells could not attch to regular polystyrene flasks but immortalised cel - does anybody know what could be happening (reply: 1)
  1465. How to culture cells after sorting? - (reply: 3)
  1466. How to kill nerve cells in a living hydrozoans? - Trying to procude partial/complete nerveless jellyfish (reply: 3)
  1467. lifespan of primary cells in vitro - how long can different cell types survive (reply: 3)
  1468. measuring apoptosis - reduced cell proliferation due to apoptosis (reply: 1)
  1469. T cell differentiation protocols - Th1/Th2/Treg/Th17 (reply: 4)
  1470. Mitochondria extraction from human liver cells - (reply: 1)
  1471. lipofectamine and PEI transfection of hela cells - (reply: 2)
  1472. What can I do whit my Cancer Stem Cells?(I know, its a big question..) - (reply: 7)
  1473. Wash L929 Cells with DPBS (with or w/out CA MG?) - DPBS with or W/out CA,MG? (reply: 2)
  1474. Especially tenacious cells -- what to do? - (reply: 9)
  1475. MDCK cell vascuolation - I got my MDCK cell growing in the T-75 flask (reply: 5)
  1476. Culturing HeLa Cells - (reply: 2)
  1477. expression of His-tagged protein in insect cells - (reply: 2)
  1478. How to isolate vascular smooth muscle cell? - (reply: 1)
  1479. Whole cell lysate including nucelar proteins? - (reply: 3)
  1480. Colony PCR with Eukaryotic Cells? - Any Experiences? (reply: 4)
  1481. HELP with cell lysis for DNA assay - Something is floating in my lysate solution!!! (reply: 1)
  1482. culturing MCF-7 cellls - clumping and maintaining healthy cells (reply: 3)
  1483. Cell culture media - require information on media (reply: 3)
  1484. how to measure ROS in cell wiht H2DCFH - (reply: 4)
  1485. VEGF in cell nucleus - (reply: 2)
  1486. cell treatment rules - (reply: 4)
  1487. method to seed cells in hyrogels - cell seed in hydrogels (reply: 1)
  1488. transfecting human mesynchemal stem cells - (reply: 5)
  1489. cell schock - (reply: 2)
  1490. Flow cytometry of RFP and CFP expressing yeast cells - (reply: 1)
  1491. Can you store cell pellets for future nuclear extraction? - (reply: 5)
  1492. using cell lysate to run native gel? - (reply: 1)
  1493. Cell Suspension from Frozen Tissue? - (reply: 6)
  1494. looking for stromal cell marker - (reply: 1)
  1495. 'Black dots' in cell culture - (reply: 2)
  1496. ChIP in WT and KO cells - how to analyze - (reply: 1)
  1497. Mouse T cell line - (reply: 1)
  1498. Making competent cell from freeze-thawed stock - (reply: 2)
  1499. Invasion assay using matrigel- cell clumps - (reply: 1)
  1500. producing cell lines - why viruses ?? (reply: 4)
  1501. What color should the cell pellets be after sonication and centrifugation? - (reply: 1)
  1502. Please help--possible bacteria issue--cholesterol + red blood cells involved - (reply: 1)
  1503. Cell Lysis for DNA extraction - Not every sample is successful (reply: 5)
  1504. How to detach an antibody from cell surface - (reply: 4)
  1505. Water for electrocompetent cells - (reply: 2)
  1506. Gp 293 Luc cells - (reply: 1)
  1507. how to check cell viability - (reply: 1)
  1508. Starter culture dilution (preparation of competent cells) - I don't know how much should I dilute my starter culture... (reply: 3)
  1509. cell fixation - (reply: 1)
  1510. cell detachment in IHC - can I incubate cells with hoechst, heparin and PFA at the same time? (reply: 1)
  1511. cell immobilization for confocal - (reply: 1)
  1512. problem with 293T cell co-transfection - (reply: 5)
  1513. Transfection and WB - Transfected cell line shows incorrect bands on WB (reply: 2)
  1514. Cell Death after formaldehyde treatment - Immunofluorescence (reply: 5)
  1515. Cell Densitometry - (reply: 1)
  1516. My cells died after changing media... - (reply: 6)
  1517. Help for an Organic Chemist - Question about bacteria cell wall permeability. (reply: 6)
  1518. H9C2 cells - trouble when culturing H9C2 cells (reply: 4)
  1519. Contamination of cell ?? - (reply: 2)
  1520. Stem cells, primary culture - Literature - What are some good papers to read to get started in this area? (reply: 3)
  1521. nonspecific band in transfected c-abl cells - nonspecific band in transfected c-abl cells (reply: 1)
  1522. Passaging Cells - (reply: 4)
  1523. Modifying collagen stiffness / rigidity in 3d cell culture - (reply: 1)
  1524. Mysterious cell death - need Hela Help! - (reply: 1)
  1525. NSC-34 Cells sloughing off at media change - (reply: 4)
  1526. Chosing the right competent cells - (reply: 1)
  1527. Cell proliferation assay for LNCaP cells - (reply: 4)
  1528. liquid nitrogen in cell culture - (reply: 2)
  1529. differential cell counting in mixed cultures - (reply: 1)
  1530. Questions about animal cell cultue Please answer - (reply: 1)
  1531. immunofluorescence for HUVEC - how to do immunofluorescence for cancer cells (reply: 3)
  1532. membrane type of cell culture filtration - (reply: 3)
  1533. Critical column of media above cells for access of oxygen - (reply: 1)
  1534. EDTA bacterial cell lysate - (reply: 2)
  1535. Is MTS Assay suitable for Adherent cells? - Confused about MTT Assay and MTS assay.. (reply: 1)
  1536. membrane damaged = cell death? - (reply: 2)
  1537. 293 cells help! - (reply: 1)
  1538. stable cell line - (reply: 2)
  1539. Color of cryopreserved cell line - (reply: 1)
  1540. Established (tumor) cell line - or not...? - (reply: 3)
  1541. Culturing cell in absence of any growth factors - Cell culture minus growth factors (reply: 1)
  1542. proliferation assay for small cell population - (reply: 2)
  1543. Cell fixation with glutaraldehyde - (reply: 2)
  1544. Yeast contamination in cell culture - hood to blame? - (reply: 6)
  1545. Comparing cell # or DNA content - of pelleted cells (reply: 1)
  1546. bicarbonate in cell culture: powder v. liquid? - (reply: 3)
  1547. cell adhesion assay - collagen (reply: 1)
  1548. To break cell clumps - suspension culture related (reply: 4)
  1549. Problems for MTT in SH-SY5Y cell line - (reply: 1)
  1550. cell stainer size - (reply: 2)
  1551. FUNGI SILENCING - PROTOCOL TO TRANSFORM FUNGI CELLS (reply: 2)
  1552. Difficulties with the transfection of cells CHO DG44 - (reply: 9)
  1553. contamination in establishing stable cell lines - (reply: 1)
  1554. How much cell medium for 1 week of culturing? - (reply: 1)
  1555. p53 +/- matched-pair tumor cell lines? - (reply: 2)
  1556. Tranfection cell density and overgrowth - (reply: 3)
  1557. shRNA selection in suspension cell culture - (reply: 6)
  1558. trouble with plasmid transformation in BL21DE3 E. coli cells - (reply: 9)
  1559. Ethanol/methanol/dmso for cell culture - (reply: 1)
  1560. stored cytokines from supernatant cell culture - stored cytokines from supernatant cell culture (reply: 3)
  1561. Rapid Cell Count (in vivo) - (reply: 5)
  1562. cell migration assay (corning transwell) - (reply: 2)
  1563. cell culture in 2 stack flask - (reply: 1)
  1564. Invitogen cell countess - (reply: 5)
  1565. Hypoxia Treatment For Cell Culture - (reply: 4)
  1566. Picking strain for e coli competent cells - (reply: 6)
  1567. cell death - can anybody tell me what process this looks like (reply: 3)
  1568. Liver cancer cell transfection via lipofectamine - Liver cancer cell transfection method (reply: 3)
  1569. Marker do discriminate cell cycle phases - G2 phase / Mitosis (reply: 3)
  1570. hematopoietic cell lines - (reply: 3)
  1571. Cos7 Cells to produce retrovirus - (reply: 2)
  1572. how to make nonadhensive cells attach to coverslips without affecting cellular s - (reply: 1)
  1573. Constructing puromycin kill curve - How to count the number of adherent cells without detaching them? (reply: 3)
  1574. stable cell line generation - (reply: 5)
  1575. histological staining for plant cell wall components - (reply: 1)
  1576. My adherent cells are detaching - (reply: 2)
  1577. Cell culture media - DMEM and DMEM/F-12 (reply: 1)
  1578. Cell Cycle analysis of proliferating lymphocytes - (reply: 1)
  1579. Problems with HEK293 cells in 96 well plate format - 293 cells do not adhere well to the plate (reply: 1)
  1580. Cell Synchronization - (reply: 1)
  1581. Slow cell culture growth? - (reply: 3)
  1582. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
  1583. cell lysis buffer - (reply: 1)
  1584. cell aging - cell culture (reply: 2)
  1585. cell concentrations - (reply: 4)
  1586. Floating cells...pls help - (reply: 6)
  1587. Cell migration assay with 3 cell types - (reply: 1)
  1588. Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
  1589. How many B cells do I need to get a 1mg of protien - (reply: 2)
  1590. Do histology staningings work in cell culture? - (reply: 2)
  1591. Stimulating/Drugging Mammilian Cells - What is the proper way to add drugs or stimulants to a cell culture? (reply: 3)
  1592. T cell transduction - (reply: 3)
  1593. compound that block DNA replication in human cell - (reply: 1)
  1594. Tranfection of HL 60 cells - (reply: 1)
  1595. mouse liver cell line? - (reply: 1)
  1596. Failed Transformation of Tuner (DE3) cells - (reply: 1)
  1597. Cells suddenly died after changing the medium... why? - (reply: 4)
  1598. type of rat hepatic cell for toxicology study - seeking opinion on what type of cell to be used (reply: 1)
  1599. Food colouring agar plates - Do you think it will affect cells? (reply: 11)
  1600. CHO Cell contamination - (reply: 6)
  1601. solve MCF-7 culture trouble - (reply: 4)
  1602. MDCK cells vacuolation - Vacuolation of MDCK cells while staining (reply: 4)
  1603. seperating cells and antibodies - Separating cells and antibodies using centrifugation (reply: 1)
  1604. Neuronal precursor cells - (reply: 1)
  1605. Sticky cells - (reply: 2)
  1606. Having problem establishing stable cell line...... - (reply: 5)
  1607. Transferring mamallian cell colonies for selection - (reply: 2)
  1608. Antibody titration and Cell number - (reply: 1)
  1609. Cell Invasion Assay - possible to analyze with flow cytometry? (reply: 5)
  1610. Cell culture contamination - "outsider I.D." (reply: 2)
  1611. Alternative media for PC-12 cells - (reply: 2)
  1612. viability of adherent cells - (reply: 3)
  1613. How long can viably frozen cells stay good in -80 degree? - (reply: 5)
  1614. Trypsin the cell killer - How much death does trypsin actually cause (reply: 2)
  1615. How to grow cell in the iron-deficient environment? - 4T1 cell. (reply: 1)
  1616. HeLa cells and BCL 2 expression - (reply: 1)
  1617. B104 cells - (reply: 2)
  1618. SH-SYS5 cell culture for dummies... - (reply: 4)
  1619. collagen staining in cell culture - (reply: 5)
  1620. Hematopoietic lineage depletion kit - Hematopoietic stem cell enrichment kits (reply: 4)
  1621. changing cell shape - (reply: 2)
  1622. how to change cell shape? - (reply: 3)
  1623. About T-antigen and tumor cell lines - (reply: 2)
  1624. Sub-culture of N2a cells - (reply: 1)
  1625. Haematopoietic stem cells - (reply: 5)
  1626. CHO cell line - To use or not to use - - incubator ran out of CO2 (reply: 1)
  1627. proteomics - minimum cell number (reply: 1)
  1628. Standard Curve for OD Turbidity vs Cell density - (reply: 6)
  1629. Cells to Ct RNA isolation kit (Ambion) - (reply: 1)
  1630. Plasmids for protein expression in mammalian cells - (reply: 2)
  1631. cell lysis after induction - (reply: 1)
  1632. competent cell preparation? - (reply: 1)
  1633. One Shot® ccdB Survival™-T1R Chemically Competent Cells: Gateway cloning???? - problems using competent cells for destination vectors in gateway clon (reply: 5)
  1634. Dendritic cell infection - (reply: 2)
  1635. Competent Cells - (reply: 5)
  1636. looking for bovine skeletal muscle cells - (reply: 5)
  1637. my cell line doesnt change the colour of the media - even when cells are 100 % confluent (reply: 4)
  1638. Dead Dendritic cells ... please help - (reply: 1)
  1639. Cell concentration Question - (reply: 3)
  1640. cho cell line adoptation to serum free medium - (reply: 1)
  1641. Problems with viability of Jurkat's cells - (reply: 3)
  1642. THP-1 cell surface markers for Flow Cytometry - Need a robust marker for undifferentiated THP-1 cells (reply: 1)
  1643. 24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
  1644. Gene expressed in lenti-packaging cells, but not in lenti-infected cells - (reply: 2)
  1645. Culturing U2OS cells - (reply: 1)
  1646. staph cell count - (reply: 5)
  1647. What kind of speed for HepG2 cells. - (reply: 2)
  1648. Serum free culture media 293T cells? - (reply: 1)
  1649. Embryoid bodies - How to differentiate mouse ES cells quickly and efficiently (reply: 8)
  1650. cell count - is it right? (reply: 3)
  1651. Haemocytometer cell count - Coverslips (reply: 4)
  1652. Growing two cell types in the same flask?? - (reply: 1)
  1653. Cell isolation - (reply: 1)
  1654. trouble to grow cells on top of coverslip - (reply: 6)
  1655. about measuring luciferase activity in living cells - (reply: 8)
  1656. Culture conditions for cell treatments - (reply: 4)
  1657. preadipocyte 3T3-L1 cells question? - (reply: 1)
  1658. SH-SY5Y cell culture - Suspended cells (reply: 4)
  1659. Magnetic-activated cell sorting ? - (reply: 4)
  1660. mRNA level in transgene cell line or knocking down cell line - (reply: 3)
  1661. How to increase IF signal in acetone/methanol fixed cells? - (reply: 5)
  1662. Macrophage cell culture - Strange particles persist in an otherwise healty looking MF culture (reply: 5)
  1663. sheep red blood cell rosetting protocol - (reply: 1)
  1664. Tranformation cell harvesting - (reply: 5)
  1665. self-renewal Vs proliferation - stem cells (reply: 3)
  1666. Plyse cell and DH5a - (reply: 1)
  1667. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
  1668. INS-1 cell line - (reply: 1)
  1669. Central cell seeding - (reply: 4)
  1670. please help with Freezing primary cells - (reply: 1)
  1671. COMPETENT CELL PREPARATION - (reply: 6)
  1672. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
  1673. suggest cells expressing low levels of adhesion molecules - (reply: 1)
  1674. Cell Culture and Pipette Tips - (reply: 4)
  1675. Flow cytometry CD14 staining very dim - Bone marrow cells CD14 staining for flow cytometry (reply: 3)
  1676. Cell counting issues - the ring of death on my flasks (reply: 5)
  1677. Isolate plasmid from eukaryotic cells after transfection - (reply: 2)
  1678. Which cell line culture has endogenous Wnt1 expression? - (reply: 2)
  1679. RNA Isolation - RNA isolation from human cell lines (reply: 2)
  1680. Cell Fixation - methanol fixation of cells (reply: 3)
  1681. How to retrieve my human blood immune cells from liquid nitrogen without killing - (reply: 3)
  1682. stem cells and EB (Embryoid bodies) - (reply: 1)
  1683. Immunohistchemistry of red blood cells - (reply: 1)
  1684. cell cryosection - (reply: 5)
  1685. Shifting of entire cell cycle to the left - (reply: 3)
  1686. freezing suspended cells - (reply: 7)
  1687. Cell Brakeage...how will I know - I need the mitochondria. (reply: 1)
  1688. How many cells... - ...gives you how much RNA? (reply: 2)
  1689. Cell culture - Culture medium (reply: 1)
  1690. Anybody ever use whole blood plasma serum stimulate cells? - (reply: 2)
  1691. How can I separate my cell line from feeder cells - (reply: 3)
  1692. ChIP-seq: Minimum number of cells - (reply: 3)
  1693. CHO or VERO? - Cell culture (reply: 2)
  1694. Media for squamous cells - (reply: 3)
  1695. how to trace single cell movement throughout gastrulation of zebrafish - (reply: 4)
  1696. Oxygen diffusion through cell culture medium - (reply: 2)
  1697. Request for Mono Mac-1 cell line - (reply: 1)
  1698. help: bovine Intestinal Epithelial Cell Culture protocol - (reply: 4)
  1699. How much sonication power needed? - protein extraction from cells (reply: 2)
  1700. protein extraction - from cell line in T25 flask (reply: 5)
  1701. Getting cells from flasks - (reply: 5)
  1702. transformation efficiency decreased - storage conditions of competent cells (reply: 7)
  1703. Fixation of immune cells - "how to" protocol needed (reply: 2)
  1704. cell cycle analysis by FACS - (reply: 2)
  1705. Variable expression of antigen in various subcellular locations - Any ideas why? - ICC Staining on Glioma Cells (reply: 2)
  1706. cell cycle analysis PI staining shift - (reply: 1)
  1707. Lipofectamine transfections once working now dying - Optimised transfection in HEKs starting to cause cell death (reply: 6)
  1708. Problem with stable overexpression cell lines - (reply: 3)
  1709. seeking help in stable cell line generation - (reply: 3)
  1710. SDS, Cell numbers, dilution - (reply: 18)
  1711. Help with Hep G2 cells - (reply: 1)
  1712. Floating cells in adherent VERO culture - contamination? (reply: 2)
  1713. RAW264.7 cells+nitric oxide determination - (reply: 13)
  1714. BL 21 cells - (reply: 4)
  1715. Mutated MDCK cells - protocol for mutating MDCK cells (reply: 1)
  1716. Preparing mutated MDCK cells - method of mutation of MDCK cells (reply: 1)
  1717. Cell pellet pesuspension without Vortexing or pippeting - seeking advice - (reply: 2)
  1718. western blot problem: cell culture VS mouse tissue - (reply: 4)
  1719. How long are cells viable in PBS? - (to make trypan blue exclusion assay less tedious) (reply: 2)
  1720. Detachment of very adherent cells for flow cytometry - (reply: 7)
  1721. THP-1-Blue-CD14 cells - (reply: 4)
  1722. MCF-7's not responding to estrogen - (reply: 5)
  1723. are cells prone to swelling in PBS after sorting - (reply: 3)
  1724. Is it Possible - Is it possible RNAi cross-reaction with normal cells molecules?? (reply: 1)
  1725. suspension cell culture - optimum cell number in plates (reply: 2)
  1726. Whole tissue starting product - not cultured cells - (reply: 2)
  1727. DNA extraction fron HSV-1 infected Vero cell - (reply: 2)
  1728. How fast do DH5-alpha cells with plasmid insert lyse after defreezing? - (reply: 1)
  1729. Overnight SURE cell culture in LB-carbenecillin - (reply: 4)
  1730. culturing COS-7 cells - (reply: 5)
  1731. Would transfected plasmids bind histones in HeLa cells? - h3k4 demethylase LSD1 (reply: 3)
  1732. Loss of cell adherence after transfection - The first passage after selection process cells lost adherence (reply: 2)
  1733. how will i be able to culture tumor cells? - (reply: 3)
  1734. General cell lysis question - whole cell lysates need to be centrifuged? (reply: 2)
  1735. Rnase for cell cycle - (reply: 5)
  1736. Transfection Issue - Are MCF-7 & MDA-MB-231 cells generally difficult to transfect? (reply: 2)
  1737. cell loss upon centrifugatino - (reply: 10)
  1738. cell lines vs tissue expression - (reply: 1)
  1739. Is molecular biology grade DMSO good enough for cell stock? - (reply: 2)
  1740. Clonogenic assay with agressive cell line - (reply: 4)
  1741. e coli cell lysis - heterologous gene expression in e coli (reply: 3)
  1742. Number of cells in 6 wells-plate well - (reply: 1)
  1743. protein extraction from primary cells - (reply: 1)
  1744. Extracting proteins from cells - (reply: 1)
  1745. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
  1746. strange dots in cell culture flask?? - U87 cell line culture has strange white dots (reply: 6)
  1747. cell floating in 96-well plate - (reply: 1)
  1748. Inducing Adherence in Suspension Cells - (reply: 1)
  1749. bacterial contamination in cell culture - (reply: 3)
  1750. THP-1 cell culture - (reply: 1)
  1751. H9c2 cell contamination? - (reply: 4)
  1752. Recovery plasmids from dead bacteria - How can i rescue my plasmid from dead cells??? (reply: 4)
  1753. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
  1754. transfection in floating cells - (reply: 5)
  1755. immortal or stable cells - primary human cells to immortal cell line? (reply: 3)
  1756. SDS-PAGE Cell lysis problems - (reply: 3)
  1757. Storage of invitrogen top10 cells - (reply: 11)
  1758. oxidative stress - how to measure oxidative stress in cells (reply: 2)
  1759. Cells clots forming during preparation mouse splenocytes - (reply: 4)
  1760. Transwell Cell Migration/Invasion Assay - (reply: 4)
  1761. neuronal nitric oxide synthase/cell line - (reply: 3)
  1762. HL 60 cells passage - (reply: 1)
  1763. Establishing a new cell line - (reply: 1)
  1764. Isolating Natural Killer cells from whole blood - (reply: 2)
  1765. Centrifugation of epithelial cells - (reply: 2)
  1766. cell engineering exam - (reply: 5)
  1767. Cell detachment: alternative to trypsine/EDTA? - (reply: 3)
  1768. Cell supplier - (reply: 2)
  1769. Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (reply: 6)
  1770. Mir34a positive control cells - (reply: 4)
  1771. Medium for MDCK cells overexpressing MRP-1 - (reply: 1)
  1772. lentiviral transdcution of hematopoëtic stem cells - (reply: 2)
  1773. FACS positive cells in negative controls help - (reply: 1)
  1774. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
  1775. How to separate fungus cells from human lung tissues - (reply: 7)
  1776. Growing of TOP10 chemically competent cells - (reply: 4)
  1777. Stable Cell Selection with Neomycin - (reply: 4)
  1778. endogenous miRNA of cos-7 cell - (reply: 2)
  1779. Qustion about MDA-MB-157 cells culture - (reply: 4)
  1780. Folic acid solution for cell culture - (reply: 2)
  1781. Strange dots around the cell+Photo - (reply: 6)
  1782. FACS of GFP expressing cells from mice brains - (reply: 1)
  1783. cells can't attach in the flasks - (reply: 4)
  1784. Differential expression of 3 adenoviruses in different cell lines. Why? - (reply: 3)
  1785. Cell fractionation and isolation of nuclei - (reply: 1)
  1786. Methanol toxicity in cell culture - (reply: 15)
  1787. Freezing cells after fix & perm and also a BrdU question - (reply: 4)
  1788. Freezing cells, quick question - (reply: 1)
  1789. Best place to buy cells - (reply: 2)
  1790. help on generation of stable cell lines with pBabe vector - pBabe-puro, phoenix Ampho cells (reply: 7)
  1791. DMSO usage in cell culture - (reply: 9)
  1792. stopping brief cell treatments - (reply: 2)
  1793. Phenol red in media - What metabolic products of cells are acidic? (reply: 2)
  1794. Cell culture container - (reply: 2)
  1795. cell lysis method - (reply: 1)
  1796. Dendritic cell immunology - activated naive cells? (reply: 9)
  1797. cell contamination???? - (reply: 1)
  1798. counting number of cells - (reply: 6)
  1799. Stacking of RAW 264.7 cells - (reply: 1)
  1800. retroviral generation of stable cell line - cells just don't seem to be growing (reply: 3)
  1801. Lentiviral transduction of B cells - (reply: 7)
  1802. Lentivirus transduction of spleen cells - (reply: 2)
  1803. trouble with establishing a stable cell line - (reply: 3)
  1804. Cell culture conditions and antigen expression - (reply: 1)
  1805. Phoenix packaging cell and diphtheria toxin - (reply: 3)
  1806. Gene expression in cell line - (reply: 1)
  1807. isoflurane and FACS analysis of peripheral blood? - my cells look terrible everytime I use isoflurane to anaesthetize (reply: 5)
  1808. Growing Up Cells - (reply: 3)
  1809. protein extraction after cell lysing - (reply: 2)
  1810. cell line for neurodevelopment - (reply: 2)
  1811. Difficulty in doing hemocytometer cell count - (reply: 4)
  1812. No RNA from cell pellet using Qiagen MiniKit!!??!! - (reply: 5)
  1813. Method for lysing cells for fractionation - (reply: 4)
  1814. Neuronal cell culture - why use neonatal brains? - (reply: 1)
  1815. Detect gene in stable cell line, - (reply: 4)
  1816. staining cells - (reply: 3)
  1817. pIRESneo for mammalian cells transfection - (reply: 4)
  1818. Protocol for staining live cells - Staining live cells for Flow (reply: 2)
  1819. H9C2 - cell line - Need clarification (reply: 1)
  1820. Cell plating calculation !! - Help needed with Cell plating calculation !! (reply: 2)
  1821. Cell lines to study epigenetics - (reply: 2)
  1822. How to transfect two plasmids in a cell - (reply: 10)
  1823. Cells dying before i can get them to in vitro assay - (reply: 1)
  1824. Strange bands in western of RPE-1 cells - (reply: 1)
  1825. Semisolid Medium For HL 60 cells - (reply: 4)
  1826. Cell culture contamination (do not know what it was) - (reply: 1)
  1827. questions about cell lysis - (reply: 7)
  1828. Gateway Cloning Problem - Lawn of E. Coli Cells - (reply: 4)
  1829. How to homogenize cell without disruption of nuclei? - (reply: 2)
  1830. Harvesting cells for flow cytometry - (reply: 10)
  1831. culture C6/36 insect cell lines - (reply: 1)
  1832. seeding cell densities - seeding cells at same density (reply: 1)
  1833. cell culture best practices - virus immortalized and non-immortalized cells (reply: 1)
  1834. Culturing QT6 cell line - Ideal culture conditions (reply: 2)
  1835. Calculating MOI for luciferase-transducted cells. - Would the sensitivity of a fluorescent microscope be enough to see the (reply: 3)
  1836. Picking epithelial, endothelial and neural cell lines - Please suggest best models (reply: 2)
  1837. How to fix suspencion cells for DAPI detecting under the microscope? - (reply: 6)
  1838. primary lung epithelial cells - (reply: 1)
  1839. Generating double stable cell line - (reply: 3)
  1840. HACAT cells - very low cell viability after thawing cells (reply: 18)
  1841. cell line for luciferase assay - How to choose the best cell line? (reply: 2)
  1842. My cells suddenly die !!! - Please help me ! why is this ? (reply: 11)
  1843. database for mutations in cancer cell lines? - (reply: 2)
  1844. Sonication Problems, Branson 450, LNCaP cells - (reply: 4)
  1845. ? Definition of Passage for suspension cells - (reply: 1)
  1846. Direct RT-PCR from Frozen Cells? - Ever try this? (reply: 1)
  1847. different staining intensity - fixed permeabilized cells and non-fixed cells (reply: 2)
  1848. Chip problem: antibody or cell number?? - (reply: 6)
  1849. stable cell line - (reply: 1)
  1850. microbial cell viability - any recommendations - assays (reply: 1)
  1851. SF9 cells not adhering - (reply: 10)
  1852. HeLa cells - Growth of thawed HeLa cells (reply: 7)
  1853. Dendritic Cells and Flow Cytometry - (reply: 11)
  1854. direct cell lysate - (reply: 8)
  1855. How long can I wait after seeing HeLa cells till transfection - will it affect my Efficiency (reply: 6)
  1856. Culturing/Mounting of polarised cells for confocal microscopy - (reply: 1)
  1857. Intestinal cell culture: Protocol required - (reply: 1)
  1858. Carbon di oxide free cell culture - (reply: 1)
  1859. surface staining of THP-1 cell - (reply: 2)
  1860. How to fix GFP expressing cell to preserve GFP signal in immunofluorescent stain - (reply: 3)
  1861. Cell Wall Lysis For Dummies - (reply: 4)
  1862. Tissue & Cell Fixation solutions - (reply: 3)
  1863. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  1864. Treating cells with chemicals and how to dilute into medium - (reply: 5)
  1865. T lymphocyte ChIPs are not working... - while other cells are! *grumble grumble* (reply: 6)
  1866. Using Real Time PCR for Cell Viability - Any tips? (reply: 6)
  1867. Has anyone used COS-7 cells for retina? - (reply: 1)
  1868. How to perform a growth curve? - i need to compare 3 cell lines for growth (reply: 2)
  1869. siRNA concentration in divinding-growing cells - (reply: 2)
  1870. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
  1871. cell culture guideline from abcam - (reply: 5)
  1872. Whole cell PCR - DNA purification without using proteinase K? (reply: 8)
  1873. problems with detecting protein expression - protein expression in cell (reply: 4)
  1874. freeze PBMC and cell sorting? - (reply: 1)
  1875. Control vehicle treatment in cells - (reply: 2)
  1876. Cancer cell culture exploded onto my face - (reply: 8)
  1877. FACS analysis of GFP expressing cells - Fix or not - (reply: 10)
  1878. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
  1879. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
  1880. Heterogeneous methylation in a same cell line - (reply: 1)
  1881. siRNA cell function examples - (reply: 1)
  1882. Good comprehensive resource for maintaining immortal cell lines? - (reply: 1)
  1883. DH5a comp cells - (reply: 5)
  1884. Trypsinized cells after fixation - (reply: 3)
  1885. Detaching Hela cells - (reply: 3)
  1886. help! Truncated protein cannot expressed in mammalian cells? - (reply: 6)
  1887. cell cycle softawe - (reply: 3)
  1888. How to maintain cells at a specific apoptosis stage - (reply: 12)
  1889. Which cell line produce cytokine? - (reply: 13)
  1890. Cell Viability - (reply: 1)
  1891. Shipping of cryopreseved cell - (reply: 15)
  1892. To what extent competent cells be thawed before transformation? - (reply: 4)
  1893. Lipofection transfection of HEK293 cells unsuccessful - (reply: 10)
  1894. Storing HEK293 cells at -80C - (reply: 5)
  1895. How to control cloned cDNA expression level in cells? - (reply: 8)
  1896. RNA isolation following apoptotic cell labeling - (reply: 3)
  1897. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
  1898. Is there an optimal incubation time for cell lysis? - (reply: 7)
  1899. The way to count adherent cells in 96 well - (reply: 3)
  1900. lysing cells in LSB - (reply: 2)
  1901. Using NIH3T3 cells of look at MAPK activation by Ras - (reply: 1)
  1902. Staining for neural crest derived cells?? - (reply: 2)
  1903. Dendritic_ cells - (reply: 3)
  1904. problem to stain just one cell line in coculture - (reply: 4)
  1905. Culturing stromal cells - (reply: 2)
  1906. A549 cells - (reply: 4)
  1907. Chikungunya Virus Culture - Chikungunya Virus culture in mammalian cells form serum sample of huma (reply: 2)
  1908. Protein extraction from cell culture media - (reply: 9)
  1909. Bovine cell line for transfection... - Transfection friendly cell line for localization studies... (reply: 1)
  1910. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
  1911. yeast gene deletion - Could I delete an essential gene from haploid cells? (reply: 8)
  1912. can we prepare Copmetent cell by calicum cloride with 0.09 OD - Cp cells with 0.09 OD (reply: 3)
  1913. electroporation of HL60 cells - (reply: 1)
  1914. Intestinal epithelial cell culture - (reply: 4)
  1915. SF9 cell transfection using lipofectamine?? - and is CMV active in this cells? (reply: 3)
  1916. Easiest cell lysis protocol - no interest in preserving proteines (reply: 4)
  1917. passage number for cell line - (reply: 7)
  1918. MOCK INFECTED CELLS - (reply: 4)
  1919. cuboidal cells - (reply: 1)
  1920. Cell count using a hemocytometer - (reply: 4)
  1921. Buffers that are good for cell culture. - need buffering capacity around pH 5-7 (reply: 3)
  1922. what is mock cell line? - (reply: 4)
  1923. 293Flp cell transfection - Passaging cells 4-6 hours after lipofectamine trasnsfection (reply: 3)
  1924. How to seed the cells evenly in 6 wells plate - (reply: 13)
  1925. knockout mouse with increase cell size - (reply: 3)
  1926. Does UV cause DNA fragmentation? - how long do I need to put cells under UV to see fragmentation? (reply: 12)
  1927. Paper on energy requirement of cells - (reply: 2)
  1928. galactose induction of yeast cells - (reply: 2)
  1929. making competent E coli DH5a cells - (reply: 6)
  1930. How could you explain these results? - cancer cells are weird (reply: 1)
  1931. strange ES cells contamination - please help!!! (reply: 1)
  1932. Hek 293 cell line with tetra/doxycycline induction - how to lower expression level? (reply: 2)
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