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DNA Related Discussions

1. Trouble with inconsistant DNA extraction - (reply: 3)
   
2. selective preciptation of DNA according to size - (reply: 4)
   
3. Tricks to amplify 4kb targets from GC rich genomic DNA - (reply: 4)
   
4. SDS vs. CTAB vs. Sarkosyl - cell lysis/DNA extraction (reply: 6)
   
5. Phenol/chloroform vs Column purification for ChIP DNA - (reply: 3)
   
6. what dose the genomic DNA electropherogram look like? - (reply: 2)
   
7. genomic DNA digestion - EcoRI+MseI - (reply: 2)
   
8. questions about isolating genomic DNA by alkaline - (reply: 6)
   
9. DNase removal prior to mRNA isolation - (reply: 4)
   
10. RNA sample precipitates when I add the 10X Buffer of the Turbo Dnase (AMBION) - RNA precipitation (reply: 1)
    
11. RNA samples precipitates when I add 10X Buffer for Turbo Dnase treatment - RNA precipitation (reply: 2)
    
12. protein/DNA methylation interaction - (reply: 5)
    
13. Best storage temperature for samples - serum, protein, tissue, DNA, RNA... (reply: 3)
    
14. Linear DNA transfection - Daudi cells (reply: 3)
    
15. Plasmid DNA concentration - (reply: 2)
    
16. Quantifying Cell Lysis or the Presence of DNA - (reply: 3)
    
17. How to deal with so many false positive clones - Transfer linear DNA into E.coli competent cells... (reply: 2)
    
18. DNA motif database / no TF - database of DNA motifs for binding of DNA replication proteins (reply: 2)
    
19. How to normalize ELISA measurements? - Total protein, cell number, or DNA? (reply: 1)
    
20. DNA precipitation EtOH with Na OAc or Ammonium OAc - (reply: 4)
    
21. Making blunt ends DNA with T4 Polymerase - (reply: 6)
    
22. A problem with plasmid DNA extraction - (reply: 6)
    
23. Cleaning up dirty gDNA (desperate...) - (reply: 6)
    
24. DNA extraction from Filters with UltraClean Soil kit. - (reply: 2)
    
25. How to extract DNA for PFGE - (reply: 2)
    
26. DNA extraction from Fruit pulp - HELP !!!! (reply: 2)
    
27. genomic DNA and cDNA - question (reply: 4)
    
28. genomic DNA and cDNA - question (reply: 1)
    
29. phenol turns pink after adding it to the sample - phenol:chloroform DNA extraction (reply: 6)
    
30. estimation of DNA mass using software - (reply: 2)
    
31. starting amount of DNA - (reply: 3)
    
32. Why DNA sequencing does not detect deletions - help! - Med student lost on her hw. Breast cancer gene (BRCA1) mutations (reply: 3)
    
33. Measuring Ethidium Bromide without DNA - (reply: 5)
    
34. Good DNA quantity with nanodrop but nothing on the gel - (reply: 8)
    
35. Sequencing non-existent DNA? - (reply: 9)
    
36. Gel band of bacteria genomic DNA - (reply: 1)
    
37. band distortion in DNA gel electrophoresis - (reply: 1)
    
38. How to reuse DNA purification columns? - (reply: 3)
    
39. Working out PEI:DNA ratio for transfection - (reply: 2)
    
40. High DNA Spec - Nothing on the Gel... - (reply: 2)
    
41. Problem with genomic DNA contamination in Minipreps - Qiagen QuickLyse Miniprep Kit (reply: 2)
    
42. Mouse Tail Genotyping: Viscous DNA - (reply: 3)
    
43. gDNA isolation Question - must use tails? (reply: 3)
    
44. Addition of Calf thymus DNA? Why? - (reply: 2)
    
45. Small DNA fragments separation - (reply: 2)
    
46. Why am I only getting smears for my genomic DNA long-distance PCR products? - It worked great with sheep BACs but failed miserably for genomic DNA (reply: 4)
    
47. dna sequencing question - basic question (reply: 1)
    
48. Separating a 2750 base DNA band from a 2600 base DNA band - (reply: 8)
    
49. Inconsistent ChIP PCR - PCR bands vary from same DNA sample (reply: 3)
    
50. DNAse inhibitors - (reply: 1)
    
51. wierd dna sequencing results - any clues? (reply: 4)
    
52. DNA laddering - apoptosis - any suggestion about the extraction procedure ? (reply: 1)
    
53. DNA Sequencing Problem - (reply: 6)
    
54. Phenol:chloroform:isoamyl pH 5 for DNA extraction? - Kinda worried, only have pH 8 but that should be fine right? (reply: 1)
    
55. DNA pooling - (reply: 1)
    
56. How much salt can/should be used for DNA precipitation? - Is there a lower or upper limit? (reply: 8)
    
57. how to distinguish supercoiled DNA from linear one in agarose gel - (reply: 2)
    
58. DNA "snot" in cell lysis/protein extraction - (reply: 6)
    
59. Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
    
60. ChIP sonification woes - why is my DNA cut into tiny pieces?? :( (reply: 7)
    
61. DNA extraction from dry cells - How to extract DNA from dry cells (reply: 9)
    
62. Quantifying Bisulfite treated DNA - (reply: 7)
    
63. genomic DNA contamination in RT-PCR - (reply: 4)
    
64. Unmehylated control DNA - (reply: 4)
    
65. Help w/ M.B. DNA - Concentration on DNA in ug/ul (reply: 1)
    
66. Purification of RF DNA from phage prep - Need to get rid of ssDNA contamination (reply: 2)
    
67. gDNA extraction from mouse ear for PCR - "Hot Shot" extraction (reply: 1)
    
68. DNA cloning problem - (reply: 3)
    
69. No DNA in 1 of 6 plasmid preps (Qiagen - Midi) - (reply: 2)
    
70. recovering DNA from gel - and then secquencing it? (reply: 7)
    
71. analysis of big DNA fragments >50Kb - (reply: 1)
    
72. dna quantification - 260/280 vs 260/230 (reply: 1)
    
73. DNA sequencing - Problem with silver staining (reply: 3)
    
74. Denaturing DNA - (reply: 2)
    
75. Bacterial DNA at mice genotyping place of work - (reply: 2)
    
76. Strange amplification pattern in DNA real time PCR - (reply: 4)
    
77. The role of salt in DNA precipitation - (reply: 3)
    
78. Is there any way to remove chromosomal DNA from plasmid DNA? - (reply: 6)
    
79. Cell Lysis buffer....do I have to add sodium phosphate to the buffer? - lysis buffer for DNA extractions (reply: 4)
    
80. DNA Protein pull down protocol for mass spec - (reply: 1)
    
81. ng of DNA - (reply: 1)
    
82. Could have more DNA concentration inhibition effect on PCR? - (reply: 3)
    
83. DNA Gel - (reply: 7)
    
84. How to sequence cosmid DNA insert - (reply: 2)
    
85. house keeping gene not intron spanning and sample DNase UNtreated - (reply: 1)
    
86. dna sequencing problem - (reply: 8)
    
87. Cotransfection of DNA and siRNA in Neuro2a cells - (reply: 1)
    
88. qiagen dna purification columns - are they all the same? (reply: 2)
    
89. No detection of genomic DNA inDNA laddering - (reply: 7)
    
90. Erroneous PCR product due to plasmid DNA contamination - (reply: 2)
    
91. reprecipitate DNA - reprecipitate DNA (reply: 11)
    
92. DNA melting profiles - Constant Temp (reply: 1)
    
93. linearized DNA (entry clone) for LR recombination? - (reply: 1)
    
94. time of DNase I treatment - (reply: 4)
    
95. DNA elongation in PCR - Multidirectional? (reply: 2)
    
96. 260nm/280nm ratio of DNA after maxi preparation - why <1.5 (reply: 4)
    
97. Proecess after DNA replication - (reply: 1)
    
98. A reason for DNA's accuracy? - (reply: 6)
    
99. nature of DNA - (reply: 1)
    
100. DNA replication quaestions - (reply: 2)
     
101. DNA Vector - Stability at room-temperature? (reply: 2)
     
102. rhizome DNA extraction by QIAgen kit - (reply: 1)
     
103. genomic DNA PCR - is it the same as a cDNA PCR (reply: 9)
     
104. pup VS adult mice tail DNA - (reply: 4)
     
105. DNA Maxi Preparation - Viscous DNA? (reply: 8)
     
106. genomic DNA storage - (reply: 3)
     
107. gDNA isolation and get a single band - what does it mean? (reply: 1)
     
108. chromatin DNA and restriction endonucleases - (reply: 1)
     
109. Attaching DNA bases - Creating primers and plasmids? (reply: 3)
     
110. chromosomal DNA extraction E. coli - (reply: 3)
     
111. How to conduct DNA cloning if plasmid's restriciton site is un-unique - (reply: 5)
     
112. How can i extract DNA from hemolysis blood - (reply: 4)
     
113. How extraction DNA from hemolsis blood? - (reply: 1)
     
114. DNA purification after sonication - (reply: 12)
     
115. DNA methylation analysis - doubts - (reply: 5)
     
116. HELP No DNA after Nucleic Acid Extraction (Qiagen kit) - (reply: 13)
     
117. Low genomic DNA - (reply: 2)
     
118. DNA blunting - DNA blunting troubleshoot (reply: 4)
     
119. PCR components/DNA sample optimal amounts - ul of DNA and each component for a good reaction. (reply: 10)
     
120. harvest DNA or RNA from paraffinembedded tissue? - Is it possible to use a block of tissue for other analysis than IHC? (reply: 4)
     
121. Large DNA fragmnet ampification - (reply: 6)
     
122. precipitation of protein (PI=9.5) but not DNA - I need protein free sample for DNA quantification (reply: 2)
     
123. Baculovirus expression problem - Mixture of Bacmid DNA (reply: 2)
     
124. Method to extract genomic DNA from human brain - (reply: 2)
     
125. Anyone knows Components of the lysis buffer of Qiagen DNA Isolation Kit - (reply: 1)
     
126. why DNA from mouse tails are used for genotyping ? - need to understand the basic concept (reply: 3)
     
127. gel purifications of DNA fragm > 10Kb - (reply: 9)
     
128. DNA migration problem - Genomic DNA (reply: 1)
     
129. bacterial DNA isolation - various methods (reply: 1)
     
130. how to clone my insert DNA into vector? - (reply: 11)
     
131. purifying small DNA fragmets - (reply: 2)
     
132. problems cleaning up small DNA products - (reply: 2)
     
133. DNA sequencing downstream work - (reply: 3)
     
134. how to extract DNA from adenovirus for template of PCR - (reply: 2)
     
135. DNA extraction...help needed! - extracting dna from buccal cells in ethanol (i think) (reply: 5)
     
136. Carrier DNA - poly dA / poly dT / poly dA:dT - (reply: 2)
     
137. How to improve DNase efficiency? - (reply: 1)
     
138. Removing RNA from DNA during ChIP Assay - (reply: 3)
     
139. How to improve DNase efficiency after in vitro transcription? - (reply: 3)
     
140. how to do deproteinization for DNA samples? - (reply: 11)
     
141. CHIP / Is it normal to see pellet after the DNA purification? - (reply: 3)
     
142. Preserve monocytes for DNA extraction - (reply: 1)
     
143. DNA binding proteins ELISA - need suggestions (reply: 1)
     
144. gDNA template for PCR - (reply: 2)
     
145. Ladder Fine but no DNA bands please help! - (reply: 6)
     
146. Troubleshooting in quantifying Genomic/WGA DNA - (reply: 5)
     
147. DNA Dialysis - DNA Purification (reply: 1)
     
148. Dried DNA - (reply: 2)
     
149. Random DNA library problem - repair, ligation, digestion problem? TOPO and Zero Blunt (reply: 7)
     
150. DNA extraction - Brown color (reply: 2)
     
151. Retroviral calcium phosphate transfection - How much DNA should I use?? (reply: 1)
     
152. CaPO4 Transfection - How much DNA should I use?? (reply: 3)
     
153. amplifying small amounts of converted DNA or unconverted DNA? - (reply: 2)
     
154. DNA annealing help - (reply: 2)
     
155. DNA won't dissolve in water! Help! - (reply: 5)
     
156. DNA molar calculation - (reply: 3)
     
157. isolation of genomic DNA - (reply: 1)
     
158. Random-primed DNA labelling - (reply: 1)
     
159. DNA extraction vs purification - help ! (reply: 2)
     
160. Plasmid Prep Problem : gDNA - bacterial genomic DNA contamination in a plasmid minipreparation (reply: 3)
     
161. how can I dry down DNA? - (reply: 7)
     
162. DNAse I incubation - shortest sufficient time - (reply: 9)
     
163. Method for soil DNA extraction - (reply: 3)
     
164. Cloning from genomic DNA - (reply: 7)
     
165. Digesting ethanol precipitated DNA with AseI - (reply: 2)
     
166. which qiagen kit could be used in DNA recovery in ChIP assay? - (reply: 6)
     
167. cost of DNA sequencer? - (reply: 2)
     
168. GenomiPhi first-strand cDNA - Can GenomiPhi be used on single-stranded DNA? (reply: 2)
     
169. how to pull out one stranded DNA binded with histon and conserve another one - (reply: 2)
     
170. How to pull out one stranded DNA binded with histon and conserve another one? - (reply: 1)
     
171. How to purify biotin labled DNA from mix - (reply: 2)
     
172. How to pull out bottom string DNA and reserve up string DNA - (reply: 2)
     
173. DNA gel electrophoresis problem? - (reply: 5)
     
174. DNA gel to Test Ligation? - (reply: 2)
     
175. New to Transfections! Please Help! - What specifically is the DNA in my transfection protocol??? (reply: 2)
     
176. optimize TRIzol protocol for RNA/DNA/Protein, HELP? - small amout of input material (reply: 1)
     
177. complete bisulfite treatment - How do you know if your DNA is completely modified by bisulfite treatm (reply: 2)
     
178. RNA precipitation with DNase treatment - (reply: 1)
     
179. Amount of DNA to run on gel - Be it from PCR or from DNA extraction steps (reply: 4)
     
180. PCR Inhibition by DNA? - (reply: 5)
     
181. Questions in collection and preservation of plant samples - Purpose: DNA Extraction for DNA fingerprint (reply: 5)
     
182. Strange results with DNase - any explanation? - (reply: 3)
     
183. Endofree DNA precipitation accident-- can I salvage my dna? - (reply: 1)
     
184. Apoptotic DNA ladder or not? - (reply: 2)
     
185. DNA polymerase fidelity assay (NOT PCR-based) - (reply: 2)
     
186. Cesium-cleaned environmental DNA - Help! - Running into problems after extracting ethidium (reply: 6)
     
187. measuring DNA concentration in a microplate - without pathway correction (reply: 6)
     
188. Taq vs. T4 dna polymerse: difference? - (reply: 4)
     
189. Removing genomic DNA contamination -- Ambion Turbo DNase or Qiagen RNase-free DN - (reply: 4)
     
190. DNA gel problem - (reply: 13)
     
191. CDS Translation for DNA blasts - How to Blast 2 DNA sequences with amino acid translations? (reply: 4)
     
192. DNA methylation - (reply: 5)
     
193. apoptotic DNA ladder - (reply: 2)
     
194. DNA isolation from blood swap ? - help me (reply: 2)
     
195. problems with low concentration DNA templates for PCR - (reply: 5)
     
196. Help! questions on DNA pull-down assay - (reply: 6)
     
197. getting rid of genomic DNA in RNA extraction - (reply: 7)
     
198. How to find DNA sequence upstream of gene? - (reply: 3)
     
199. low concentartion of DNA after miniprep - (reply: 6)
     
200. PCR with paraffin-embedded tissue DNA samples - I have had problems to amplify DNA samples from paraffin-embedded tiss (reply: 1)
     
201. difference between subculture in incubator and shaker - on preparation of cultures for plasmid dna extraction, is there a diff (reply: 3)
     
202. Dna purification from gel - (reply: 20)
     
203. protocol to use Shrimp DNase - (reply: 2)
     
204. Extremely precious sample with degraded DNA - what to do? - (reply: 1)
     
205. Genomic DNA from S2 cells - is there an easy protocol? (reply: 2)
     
206. Second-strand synthesis - why use ligase? - DNA polI - mediated SSS (reply: 1)
     
207. Tips on PEG precipitation of DNA - (reply: 5)
     
208. HELP WITH RESTRICTION DIGEST OF PHAGE DNA - (reply: 4)
     
209. looking for a 2-in-1 kit for Genomic DNA/RNA - (reply: 4)
     
210. looking for Genomic DNA/RNA kit - (reply: 1)
     
211. siRNA transfection: DNA conc optimization problem - (reply: 1)
     
212. help with RT-PCR - DNase usage - (reply: 2)
     
213. Help: Genomic DNA PCR - (reply: 3)
     
214. PCR inhibition in FFPE DNA - (reply: 1)
     
215. Free trial kit for DNA, RNA, and protein extraction - AquaRNA - an aqueous DNA, RNA, protein extraction solution (reply: 4)
     
216. DNA manipulation programs (or webs) - (reply: 2)
     
217. test fuGENE 6 for the large DNA - (reply: 3)
     
218. DNA extraction for DNA laddering - (reply: 1)
     
219. Sonication DNA - (reply: 5)
     
220. Plasmid DNA preparation - two DNA bands in gel (reply: 5)
     
221. High concentration DNA on spectrophotometer but no band on agarose gel. WHere is - DNA from bone and teeth (reply: 5)
     
222. DNA extraction from PFA fixed cells - (reply: 2)
     
223. how to clone unstable DNA? - (reply: 4)
     
224. Streaked DNA - (reply: 1)
     
225. DNA extraction from dried tissue - (reply: 1)
     
226. using ficoll to collect mononuclear lymphocytes - IS it suitable for DNA extraction?? (reply: 10)
     
227. DNA sequencing - (reply: 1)
     
228. Weekly Decontamination Routine? - To prevent DNA contamination in a PCR lab (reply: 1)
     
229. Which is the best way of determining the concentration of DNA? - (reply: 3)
     
230. PCR on template with trinucleotide repeats - PCR tips for complexe DNA template (reply: 4)
     
231. DNA PCR template - GC content (reply: 1)
     
232. Can we bring DNA without TE-Buffer? - Help me, please (reply: 2)
     
233. dna ladder seperation in gel - (reply: 4)
     
234. Purpose of Genomic DNA hydrolysis - (reply: 2)
     
235. DNA cleanup - (reply: 1)
     
236. What are the benefits to extract DNA from tissues such as liver, heart and kidne - (reply: 5)
     
237. DNA not dissolving - (reply: 4)
     
238. Storage of blood prior to dna extraction - (reply: 4)
     
239. contamination of DNA in protein sample - (reply: 1)
     
240. Free sample of GoTaq® Hot Start or any GoTaq® DNA Polymerase - (reply: 1)
     
241. Labelling DNA for ChIP-ChIP - A couple of questions... (reply: 6)
     
242. DNA polymerase that produces blunt end and doesnt smear! - (reply: 2)
     
243. PCR on Genomic DNA from cell lysate - (reply: 5)
     
244. DNA spin column problems - Possible protein contamination and blank gel (reply: 6)
     
245. How to study promoter. - A presentation in related to DNA methylation (reply: 1)
     
246. How to dilute DNA ladder? - (reply: 2)
     
247. why biochemical identification and DNA identification shows different results? - (reply: 14)
     
248. RIPA for DNA Isolation - Can you do simultanous isolation of protein and DNA with RIPA (reply: 3)
     
249. DNA SEQUENCING - (reply: 1)
     
250. How to eliminate visous in leaves during DNA isolation - (reply: 5)
     
251. problem of DNA sequencing - (reply: 3)
     
252. No DNA after sonication for ChIP - (reply: 2)
     
253. Miniprep DNA digestion smear - (reply: 15)
     
254. TE buffer exposed in the air overnight...is it all right for use? - TO DISSOLVE DNA (reply: 7)
     
255. Genomic DNA fragment labeling by random priming - (reply: 1)
     
256. Dna Extraction from degraded blood samples - (reply: 2)
     
257. DNA not migrating from well - (reply: 8)
     
258. how to extract DNA from frozen blood ? - for help (reply: 6)
     
259. DNA frag. assay....protocol? - (reply: 2)
     
260. What is the function of acetate solution in DNA extraction for PCR used - (reply: 6)
     
261. DNA Methylation Analysis - MeDIP (reply: 5)
     
262. RIPA Buffer and DNA - (reply: 3)
     
263. Fate of plasmid - How to detect plasmid DNA on host chromosome (reply: 7)
     
264. how to check gDNA quality or amount - as title (reply: 3)
     
265. Miniprep DNA - (reply: 2)
     
266. To whom working with DNA sequencing - (reply: 2)
     
267. all the DNA gone after digestion - (reply: 8)
     
268. Difficulty resuspending DNA after precipitation - (reply: 7)
     
269. Transcript Amplification in genomic DNA PCR - (reply: 1)
     
270. sequencing vector DNA - (reply: 4)
     
271. Current for gel electrophoresis - Fried my DNA I think (reply: 8)
     
272. DNA quality for MSP? - (reply: 2)
     
273. Use of silver staining for DNA in polyacrylamide gels... - or any other helpful suggestios to improve detection! (reply: 5)
     
274. DNA extraction from serum ? - How can i do that?? (reply: 6)
     
275. substitute for Dnase away - (reply: 7)
     
276. Phenol Removal of DNA Preparation - (reply: 1)
     
277. Fungal genomic DNA extraction problem! - (reply: 7)
     
278. DNA laddering in Smooth Muscle Cells - (reply: 1)
     
279. DNA Footprinting Gel - (reply: 3)
     
280. pulsed field gel electrophoresis of genomic DNA - (reply: 1)
     
281. Genomic DNA Extraction - Frozen Tissue (reply: 5)
     
282. how to prepare c dna for cloning - (reply: 1)
     
283. Maintaining things RNase and DNase free - (reply: 2)
     
284. Need help, what's wrong with my DNA gel? - (reply: 10)
     
285. suitable length DNA for PCR? - (reply: 2)
     
286. Ordering DNA of a specific length - (reply: 4)
     
287. trouble with DNase treatment of RNA sample - (reply: 3)
     
288. how to remove contaminated RNA in DNA extract - (reply: 4)
     
289. southern blot - southern blot- restriction of genomic DNA (reply: 2)
     
290. DNA gel purification without a spin column - (reply: 4)
     
291. DNA and RNA concentration - (reply: 3)
     
292. Questions about Stratagene QickChange Multi-mutagenesis - about how many primers and template DNA size (reply: 5)
     
293. RNA sample DNA ladder- how do they correspond? - (reply: 1)
     
294. DNA from Agarose gel basic questions - Basic functions of chemicals in DNA extraction from Agarose Gel (reply: 2)
     
295. How to choose genomic DNA extraction Kit - (reply: 1)
     
296. Genomic DNA smears on gel - (reply: 5)
     
297. T4 DNA ligase still usable after a night out? - (reply: 3)
     
298. DNA isolation - PBS wash (reply: 2)
     
299. using restriction enzymes for studying DNA methylation - hemimethylation (reply: 3)
     
300. How can I precipitae DNA in 96-well-plate? - (reply: 5)
     
301. How to prepare RNase-free DNase ? - (reply: 1)
     
302. How to create a mutant DNA having point mutations sequence? - (reply: 1)
     
303. What is the funtion of gelatin in DNA extraction. - (reply: 1)
     
304. basic question about DNA - (reply: 2)
     
305. ficol method - peripheral blood prep for DNA extraction - ficol method (reply: 5)
     
306. Annealing plasmid DNA - (reply: 3)
     
307. Small DNA fragments, which electrophoresis shoudl i choose? - (reply: 10)
     
308. SOS: DNA purity problems - Low A260/230 of gel extracted DNA samples (reply: 4)
     
309. how to compare my DNA sequence with NCBI sequence? - (reply: 1)
     
310. The case of the dissapearing DNA - (reply: 2)
     
311. Effect of pH on melting temperature of DNA - (reply: 1)
     
312. High OD ratio from DNA Maxi Prep - (reply: 1)
     
313. DNA degradation from phenol-chloroform - how to improve phenol-chloroform extraction (reply: 1)
     
314. EtBr/DNA separation methods - (reply: 1)
     
315. what to resuspend the DNA pellet after DNA isolation step? - (reply: 4)
     
316. How to determine DNA sequence is from male or female? - (reply: 5)
     
317. Plasmid DNA extraction problem - (reply: 3)
     
318. Separation of DNA bands of sizes 8.86 kb and 9.5 kb - (reply: 3)
     
319. from Trizol to phenol/chloroform for DNA extraction - (reply: 1)
     
320. genomic DNA contamination during plasmid preparation - (reply: 1)
     
321. Why we don’t use large amount of sample to extract DNA by chelex method? - (reply: 2)
     
322. function of isoamyle alcohol in organic method of DNA extraction - (reply: 1)
     
323. DNA, RNA, protein acetone precipitation? - (reply: 2)
     
324. Can i introduce strand DNA(not ligate with the plasmid) into E. coli? - (reply: 2)
     
325. genome DNA doesn't dissolve in NaOH - genome DNA doesn't dissolve in NaOH (reply: 4)
     
326. what is good for capture of biotinylated DNA? - (reply: 7)
     
327. Storage Genomic DNA - (reply: 3)
     
328. Genomic DNA Storage at Room temperature - (reply: 5)
     
329. Dnase digestion after in vitro transcription - (reply: 4)
     
330. Problem for DNA extraction (Trizol) - Problem for DNA extraction (Trizol) (reply: 2)
     
331. Tandem integration of a DNA insert - How can I demonstrate that? (reply: 7)
     
332. Problem with amplifying bisulfite treated DNA from FFPE tissue - (reply: 3)
     
333. Phenol/Chlorophorm DNA precipitation after ChIP - (reply: 4)
     
334. DNA band Breakage - (reply: 2)
     
335. Real Time gDNA allelic discrimination - help needed - (reply: 3)
     
336. DNA methylation with dimethyl sulphate - (reply: 1)
     
337. DNA adsorption to polypropylene surfaces - (reply: 4)
     
338. freeze drying of genomic DNA - i want store The DNA genomic for many years what is the butter manner (reply: 3)
     
339. How do you label your amplified DNA? - If you use the Sigma WGA kit... (reply: 6)
     
340. how can I concentrate my plasmid DNA? - (reply: 3)
     
341. DNA smears after digestion - DNA smears after digestion (reply: 6)
     
342. DNase not cutting in assay - (reply: 1)
     
343. Great ligation problem - Double digested DNA insertion problem (reply: 5)
     
344. Miniprep plasmid DNA - How clean is it? - (reply: 3)
     
345. PCR inhibitors in soil DNA - (reply: 6)
     
346. Ratio of acrylamide to bis-acrylamide - different percentages for DNA and protein gels (reply: 1)
     
347. What is the best way to align multiple DNA sequences? - (reply: 2)
     
348. Best DNA sequence analysis tool? - what is your favorite? (reply: 5)
     
349. BSP to test imprinted genes DNA methylation of oocytes - no specific or smear bands! (reply: 4)
     
350. 2 week old maxiprep DNA smearing - (reply: 4)
     
351. PCR of AT-rich DNA - (reply: 3)
     
352. Number of DNA entering during transformation - How many molecules of DNA are taken up during transformation (reply: 2)
     
353. How to amplify 4.1Kb gene from genomic DNA? - (reply: 15)
     
354. Bisulfite tx'd DNA sequence analysis - what to do with CG to TA conversion - bisulfite sequencing analysis (reply: 7)
     
355. Help: RNaseA or Proteinase K or both for DNA extraction in CHIP - (reply: 3)
     
356. Acrylamide gels blurring DNA - (reply: 5)
     
357. Is it necessary to measure the amount of DNA before PCR? - (reply: 3)
     
358. ChIP has eaten all my DNA! - (reply: 1)
     
359. DNA amplification for chip-on-chip - WGA or LM-PCR or others? (reply: 12)
     
360. How to obtain pure DNA from ChIP - (reply: 3)
     
361. Agilent Bioanalyzer vs Nanodrop DNA quantitation - DNA quantitation (reply: 3)
     
362. My DNA fragment in agarose gel is in 4 degree - Can I extract the fragment and use it for cloning??? (reply: 6)
     
363. Can bisulphite treatment be used for detection of DNA methylation within bacteri - (reply: 2)
     
364. Plasmid problems - low yeild plasmid DNA (reply: 3)
     
365. double melt curves on rtPCR of DNA samples - (reply: 2)
     
366. DNA FISH problems - Help needed! (reply: 3)
     
367. Can you remove DPX mount to extract DNA off hematology slides? - How to remove DPX to isolate DNA (reply: 2)
     
368. DNA database compression - (reply: 2)
     
369. How to introduce DNA to animal cells? - (reply: 3)
     
370. PCR purification /DMSO / vanishing DNA - (reply: 2)
     
371. chip assay dna pptn - (reply: 3)
     
372. DNA disappeared after ligation - (reply: 2)
     
373. DNA methylation in leucocyte - (reply: 2)
     
374. storing a sample or its DNA extraction - which is prefered??? (reply: 6)
     
375. Restriction digestion and DNA degradation problem - Restriction digestion and DNA degradation problem (reply: 1)
     
376. Checking Insert using PCR from plasmid DNA? - (reply: 4)
     
377. What EDTA does in annealing of DNA and vector? - (reply: 2)
     
378. DNA Free RNA - Figuring out contaimination issues (reply: 4)
     
379. X-ray scanning of DNA plasmids - (reply: 1)
     
380. Reuse of DNA columns - very less yield (reply: 4)
     
381. polyacrylamide gel for DNA analysis - (reply: 4)
     
382. cleansing of mortar and pestle for reuse after DNA extraction - (reply: 3)
     
383. Digest-Ligase-PCR Problems (T-DNA fingerprinting gone wrong) - After ligating digested product and doing PCR on it i get no DNA smear (reply: 2)
     
384. plasmid DNA maxi prep - (reply: 1)
     
385. Does this buffer suitable for DNA extraction? - (reply: 4)
     
386. Need help in DNA extraction from petroleum - (reply: 3)
     
387. DNA sequence manipulation software - (reply: 5)
     
388. simultaneos DNA purification - (reply: 5)
     
389. DNA degradation while digestion. - (reply: 3)
     
390. Elute DNA from Watmann paper - I received a plasmid in the mail and cant get transfectants (reply: 2)
     
391. DNA isolation from small amount of cells - (reply: 1)
     
392. Problems with DIG DNA labeling! help! - Membrane was completely stained purple with no bands (reply: 12)
     
393. identifying genomic DNA contamination during plasmid isolation - (reply: 2)
     
394. ancient DNa and PCR - (reply: 1)
     
395. DNA Purification - (reply: 2)
     
396. Longest single DNA sequencing read? - (reply: 8)
     
397. Method of obtaining a DNA fragment for full coding sequence - (reply: 1)
     
398. Can alcohol precipitation give 100% recovery of DNA? - (reply: 3)
     
399. How much DNA probe is needed for Northern? - (reply: 1)
     
400. DNA Precipitaion - (reply: 3)
     
401. Ligase always needed for DNA circularisation ? - (reply: 5)
     
402. can i reuse the dna purification coulmn again - (reply: 1)
     
403. DNA pellet won't go into solution - (reply: 4)
     
404. difference between precipipitating DNA with ethanol and isopropanol - (reply: 4)
     
405. how to get rid of the DNA within RNA extraction - (reply: 3)
     
406. DNAse I - Degrading my DNA (reply: 2)
     
407. a quantitative DNA extraction - (reply: 1)
     
408. large size genomic DNA isolation - (reply: 4)
     
409. gDNA contamination in realtime PCR - (reply: 4)
     
410. annealing s.s. DNA - (reply: 2)
     
411. sticky and blunt DNA on gel migration - (reply: 5)
     
412. How much DNA is needed for Chip-sequencing? - (reply: 3)
     
413. Non-DNase related smear of digested DNA? - (reply: 4)
     
414. Maximum solubility of genomic DNA - (reply: 1)
     
415. Quantify DNA using other cuvette than quartz - (reply: 1)
     
416. Is DNA extraction From Tri reagent is good method - (reply: 2)
     
417. Why use 75% ethanol to wash DNA during DNA extraction? - (reply: 3)
     
418. DNA yields with the Qiagen PCR gel purification kit - (reply: 8)
     
419. How to identify transcription factors binding to an specific DNA sequence? - (reply: 5)
     
420. DNA extraction - starting from mouse ear tissue (reply: 1)
     
421. advice on DNA extraxtion: boiling method - (reply: 5)
     
422. how to purify ChIP DNA by using kit - (reply: 4)
     
423. How can I get rid of the DNA contamination when prepare my total RNA sampls for - I used the RNAqueous-4PCR (isolation of DNA free RNA kit)kit,still hav (reply: 8)
     
424. PCR- DNA and Tm - (reply: 1)
     
425. ChIP with unknown DNA target - (reply: 2)
     
426. Weird two bands of my purified vector DNA apeared on my gel ? - (reply: 5)
     
427. Primitive technique for preserving DNA - (reply: 1)
     
428. plasmid DNA purification - which kit should I buy? (reply: 7)
     
429. Templiphi amplification kit: How to determine the amount of DNA product - (reply: 2)
     
430. concentration of DNA for stable transfection with lipofectamine 2000 and the per - (reply: 7)
     
431. Is my DNA clean enough for a succesful ligation? - (reply: 15)
     
432. How to backtrack to DNA sequence from protein - (reply: 4)
     
433. DNA extraction kit - (reply: 1)
     
434. Mutation Screening - DNA Sequences... - (reply: 3)
     
435. DNA loss in digestion - I lose lot of DNA in digestion (reply: 10)
     
436. PCR from genomic DNA - (reply: 3)
     
437. Is it possible to transform competent cells with linear DNA? - (reply: 5)
     
438. Yeast ChIP Help - No DNA after sonication - - (reply: 2)
     
439. After extracting total RNA from tissue using trizol reagent and phase lock gel,h - I am going to extract bothe total RNA and genome DNA from one sample (reply: 4)
     
440. DNA Quantitation - OD at 260 and 280 wavelength (reply: 3)
     
441. Help! DNA and RNA extraction - (reply: 3)
     
442. Preference in isolation of plasmid DNA methods? - (reply: 5)
     
443. MeDIP DNA quantification - after MeDIP how can one quantify how much DNA is precipitated (reply: 3)
     
444. White background on DNA gels - (reply: 8)
     
445. non-UV measurement of DNA - (reply: 2)
     
446. purposes of uncut plasmid DNA - (reply: 4)
     
447. Getting Vaccinia topoisomerase I to nick and covalently bind plasmid DNA - (reply: 2)
     
448. using bacterial genomic DNA as template - (reply: 2)
     
449. glycerol [c] for dna in gel - (reply: 5)
     
450. Problems after gel purification of plasmids - Restrictases and ligase do not work on gel purified DNA (reply: 2)
     
451. Trouble with doing DNA Isolation and then running a PCR - (reply: 6)
     
452. Cloning small DNA fragments. Bacteria are not growing - (reply: 5)
     
453. Ligation with T4 DNA Ligase - (reply: 9)
     
454. Nde1 works on positive control, not on DNA sample - Restriction Digest (reply: 6)
     
455. How much ligase per ug of DNA? - (reply: 6)
     
456. plant genomic DNA library preparation - (reply: 1)
     
457. Troubles in PCR the converted DNA in promoter region - (reply: 6)
     
458. DNA in lysate - 293 lysis (reply: 1)
     
459. DNase Digestion and Reverse Transcription - (reply: 10)
     
460. DNA extraction Kits ((choose one)) - Fermentas and Bio Basic (reply: 5)
     
461. Wizard DNA Clean-up Kit 230 nm peak - (reply: 2)
     
462. How to remove the DNase in a RNA or DNA sample? - (reply: 2)
     
463. DNA isolation for waxing cover leaf - help me plzzz.... (reply: 1)
     
464. plasmid DNA not digested - (reply: 3)
     
465. DNA methylation direct kit - (reply: 2)
     
466. Produce DNA ladder marker - (reply: 3)
     
467. Need large amounts of pure viral dna - (reply: 2)
     
468. genomic DNA for qPCR quantification - target sequence? - (reply: 1)
     
469. prepare genomic DNA - (reply: 5)
     
470. DNA Extraction for Ladder Assay - Protocols to get best Agarose gels (reply: 4)
     
471. DNA Extraction Kits from blood samples - (reply: 1)
     
472. Sodium Citrate in DNA purification - (reply: 4)
     
473. Quick T4 DNA ligase - optimizing the condition for quick ligase (reply: 6)
     
474. Ammonium acetate precipitation of DNA - Help is neede for ammonium acetate precipitation of DNA (reply: 6)
     
475. DNA purification technique - After a PCR (reply: 8)
     
476. plant genomic DNA isolation and PCR - does CTAB affect PCR ? (reply: 4)
     
477. Help with DNA precipitation - (reply: 1)
     
478. T4 DNA ligase Buffer - (reply: 3)
     
479. T4 DNA ligase - (reply: 1)
     
480. Is this genomic DNA? - or plasmid? (reply: 11)
     
481. Microarray with genomic DNA and cDNA at the same time - (reply: 1)
     
482. what is the maximum DNA I can load - Southern blot problem (reply: 3)
     
483. formamide and DNA denaturation - (reply: 1)
     
484. RNA contamination: how to get ride off it from extracted DNA? - (reply: 2)
     
485. Large Scale Extraction of Bacteria Plasmid DNA - (reply: 1)
     
486. White residue after DNA extraction instead of DNA? - Where's my DNA gone?? (reply: 7)
     
487. mtiochondrial DNA: Is introns included? - Is there any introns in mtDNA? (reply: 2)
     
488. Stability of cut DNA - (reply: 8)
     
489. Cloning - Bacterial gDNA in minipreps? (reply: 6)
     
490. gDNA PCR - for promoter amplification (reply: 1)
     
491. DNA extraction with Phenol:Chloroform - DNA extraction (reply: 4)
     
492. problem in DNA extraction from glycerol-ethanol embedded tissue - (reply: 1)
     
493. How long can the genomic DNA store at 19C? - (reply: 8)
     
494. what is the best dna methylation/modification kits? - newbie kit comparison (reply: 18)
     
495. How do you consider DNA degradation? - (reply: 9)
     
496. plasmid DNA Digestion - (reply: 5)
     
497. Confused about cre-lox recombination system - Why is CreatorTM DNA cloning better over other cre-lox systems? (reply: 1)
     
498. Why the he..... is the results so differnet? - Picogreen vs. nanodrop DNA quantification of buccal swabs (reply: 7)
     
499. DNA pellet - (reply: 1)
     
500. DNA Degradation - (reply: 2)
     
501. Phenol/Chloroform extraction - What's in the aqueous phase besides DNA? (reply: 1)
     
502. inverted phenol and water phase during DNA isolation? - (reply: 5)
     
503. T4 DNA ligase - (reply: 1)
     
504. how to determine DNA concentration.....when.......... - (reply: 5)
     
505. genomic DNA extraction - for PCR (reply: 1)
     
506. I kb DNA ladder - (reply: 10)
     
507. any kit or reagent for extracting both DNA and RNA from the same cell culture - (reply: 3)
     
508. Help me for DNA concentration for PCR - (reply: 4)
     
509. Gel purification of DNA bands-any alternates? - (reply: 4)
     
510. Digested DNA cleaning - My DNA is in dh2o and I need it to be in TE for the phenol/chloroform (reply: 6)
     
511. DNA - (reply: 1)
     
512. How much DNA in PCR? - (reply: 6)
     
513. PCR problems when amplifying sequence from gDNA - amplification of ~1.2 kb from gDNA (reply: 7)
     
514. PCR problems - Fungal DNA (reply: 6)
     
515. FACS PI staining for DNA content analysis - instrumental settings (reply: 1)
     
516. dissociation curve converted dna - (reply: 1)
     
517. Extracting DNA from hair without roots - (reply: 4)
     
518. 95% ethanol vs 100% ethanol for DNA extraction - (reply: 4)
     
519. calculating the amount of DNA in the final concentration - DNA extracted from bone powder (reply: 1)
     
520. How can I calculate the copy number of plasmid DNA after mini-prep? - (reply: 5)
     
521. Dilute DNA concentration - (reply: 2)
     
522. Bisulfite modification of DNA from paraffin embedded tissue - (reply: 4)
     
523. Getting rid of RNA & RNAse contamination in DNA sample for PCR - (reply: 8)
     
524. Genomic DNA extraction - viscosity? - (reply: 3)
     
525. any experience with dna extraction from fleas? - (reply: 1)
     
526. Phenol contamination - can't get the phenol out of my DNA sample (reply: 8)
     
527. DNA binding dye info - (reply: 1)
     
528. how to resuspend or reextract DNA - (reply: 7)
     
529. Can a single restricted vector spontaneously ligate back to a circular DNA? - (reply: 3)
     
530. Problem: Plasmid DNA extraction from S. aureus - (reply: 6)
     
531. How to calculate DNA concentration after electrophoresis - hello (reply: 5)
     
532. ChIP on chip Affymetrix - Ampification protocol - Quality of amplified DNA (reply: 5)
     
533. genomic DNA extraction - more about Trizol (reply: 3)
     
534. DNA contamination in microRNA sample - How to remove the genomic DNA from microRNA sample? (reply: 5)
     
535. DNA isolation from Dendrobium - (reply: 7)
     
536. Does aza affect DNA/RNA quality? - (reply: 6)
     
537. Trouble sequencing DNA from mutagenesis reaction - (reply: 1)
     
538. How get GFP signal without mRNA target from pIRES-EGFP - positive by FACS but no DNA amplifation from RT-PCR (reply: 8)
     
539. help with hiperthermophiles - cant break them for DNA extraction (reply: 3)
     
540. sodium acetate for DNA precipitation - (reply: 1)
     
541. Real-time PCR to analyze DNA methylation - (reply: 3)
     
542. positive control for DNA ladder formation - (reply: 1)
     
543. which reagent adds density to DNA? - 3X loading dye (reply: 4)
     
544. DNA lost during wash - (reply: 4)
     
545. how to remove biotin from DNA - (reply: 3)
     
546. siRNA + DNA transfection - (reply: 3)
     
547. DNA isolation - TRizol reagent (reply: 6)
     
548. PAGE gel DNA band shape - (reply: 4)
     
549. DNase treat my RNA prior to Generacer exp? - (reply: 1)
     
550. how to get DNA sequences starting from the primer - (reply: 4)
     
551. Genomic DNA hybridization for species identification - (reply: 2)
     
552. Genomic DNA isolation - (reply: 5)
     
553. Sequencing a 10kb length of dna in a 12kb plasmid - (reply: 3)
     
554. ChIP DNA concentration - (reply: 7)
     
555. DNA clean up kit versus conventional method - (reply: 2)
     
556. DNA extraction from gel - (reply: 16)
     
557. genomic DNA isolation from hair - (reply: 3)
     
558. Smallest amount of DNA per well and can still be visualized? - (reply: 3)
     
559. Easy protocole for DNA sequencing - sequencing 1kb fragment (reply: 3)
     
560. Does my protein degrade during DNAse I and thrombin digestion? - (reply: 1)
     
561. Global DNA methylation - [3H]dCTP method - (reply: 2)
     
562. primers for bisulfite-modified DNA: general questions - (reply: 6)
     
563. Pineapple DNA Extraction - Very low or no yield (reply: 6)
     
564. DNA Extraction-pigment contamination - (reply: 6)
     
565. DNA extraction from soil - (reply: 2)
     
566. DNA isolation troubleshoot. - (reply: 2)
     
567. DNA damage - techniques (reply: 2)
     
568. RT-PCR NTC- substitute DNA with water? - or just end up with a smaller rxn volume? (reply: 1)
     
569. RT-PCR Controls (no DNA) show weird amplification - Complicated problem- urgent help needed! (reply: 4)
     
570. DNA sequencing on the left and right chromosome - do we assume that left and right chromosomes have the same DNA sequenc (reply: 2)
     
571. DNA degradation in BAC preps - DNase inhibitor? (reply: 16)
     
572. Next step after DNA methylation - What is the next step? (reply: 8)
     
573. How long can U store DNA Sample - DNA Purification (reply: 3)
     
574. Problems in the DNA extraction from blood of rats - (reply: 5)
     
575. Difficulty Isoalting DNA From Adipose Tissue - (reply: 1)
     
576. ChIP _ genomic DNA isolation - (reply: 1)
     
577. DNA stuck in wells - (reply: 9)
     
578. How to improve 260/280 reading for DNA? - 260/280 for purified digested plasmid is very high. (reply: 2)
     
579. Elementary Question - How do I send plasmid DNA?? (reply: 11)
     
580. Post ChIP DNA amount - (reply: 1)
     
581. DNA size related to the transfection efficiency？ - (reply: 9)
     
582. DNA assay in microtiter plate - (reply: 2)
     
583. Extract DNA from gel - (reply: 5)
     
584. Isolating intact chromosomal DNA from E. coli - (reply: 3)
     
585. Template DNA concentration - (reply: 2)
     
586. DNA storage: TE vs water - (reply: 1)
     
587. CTAB DNA extraction for Salmonella - (reply: 3)
     
588. Lambda DNA nanowires, Restriction sites and thiolated primers - (reply: 1)
     
589. PCR of methylated DNA? - (reply: 1)
     
590. Spec-ed/Loaded DNA inconsistency after digestion of mini-preps - (reply: 2)
     
591. GenScript- Free PCR Reagents and DNA Purification Kits! - (reply: 1)
     
592. O.D of DNA at 260nm - (reply: 3)
     
593. DNA Standard - DNA Standard for Calibration of spectrophotometer (reply: 2)
     
594. PLASMID DNA yield - (reply: 3)
     
595. DNA secondary structure - (reply: 1)
     
596. EMSA results have other bands in control DNA - (reply: 1)
     
597. dissolving DNA - (reply: 3)
     
598. ELISA/Dot blot for detection of Methylated DNA - (reply: 3)
     
599. RNase treatment - genomic DNA sample lost during purification - RNase treatment troubleshooting (reply: 1)
     
600. yeast genomic DNA extraction - (reply: 2)
     
601. Plasmid DNA Sequencing with Universal Primers - (reply: 3)
     
602. CHIP: No enrichment in IP DNA Relative to Input - (reply: 4)
     
603. sssI with genomic DNA - (reply: 8)
     
604. Chloroplast DNA southern blot - (reply: 1)
     
605. DNA isolation method form CSF - (reply: 2)
     
606. sodium acetate - the function of soduim acetate in precipitation of DNA/RNA (reply: 2)
     
607. contaminated gel eluted DNA fragment - (reply: 8)
     
608. Why the DNA sequence big different but protein the similarity? - such as MAOA and MAOB genes (reply: 4)
     
609. checking quality of DNA isolated - (reply: 1)
     
610. about size of genomic DNA - (reply: 5)
     
611. How many plasmid DNA copies per cell? - (reply: 1)
     
612. KOD HiFi DNA Polymerase - (reply: 3)
     
613. DNA Methylation - Tissue specific - (reply: 4)
     
614. Uneven background of DNA electrophoresis image - (reply: 4)
     
615. DNA Modification - (reply: 2)
     
616. 10X Orange G DNA loading dye - (reply: 1)
     
617. desire definition - Chromosomal vs genomic DNA (reply: 5)
     
618. Methods for checking DNA Contamination - (reply: 1)
     
619. How about 4 DNA fragments ligation and cloning - Cloning by 4 DNA fragments ligation and transformation (reply: 6)
     
620. DNA ends after sonication - Blunt ends or not? (reply: 4)
     
621. Why dpes uncut (undigested) DNA show more than one band in gel-electrophoresis? - Need to know the reason why it shows multiple bands (reply: 1)
     
622. Why does uncut dna show more than one band? - why does uncu dna show more than one band? (reply: 2)
     
623. Electrophoresis of DNA - (reply: 7)
     
624. Bacterial Genomic DNA Extraction - (reply: 2)
     
625. Gel extraction protocol for DNA purification - Any suggestion? (reply: 11)
     
626. Low DNA Extraction - (reply: 8)
     
627. supercoiled and relaxed DNA - (reply: 2)
     
628. mouse unmethylated DNA control - (reply: 2)
     
629. Thymus DNA extraction - (reply: 3)
     
630. DNA ligation - problem with purification (reply: 4)
     
631. Cloning a promoter sequence from genomic dna - (reply: 3)
     
632. dna purification - (reply: 1)
     
633. Primer sets for Bisulfite-modified and unmodified DNA - (reply: 6)
     
634. dna recovery from gel - (reply: 7)
     
635. making insert from miniprep DNA - (reply: 11)
     
636. DNA, RNA storage - (reply: 6)
     
637. why is dna run on urea pages,what is the specific function of urea in the page - (reply: 5)
     
638. small size DNA isolation - (reply: 1)
     
639. no DNA band - (reply: 4)
     
640. 100kb fragments in DNA laddering - (reply: 4)
     
641. dna on gel image help - (reply: 3)
     
642. PCR DNA IMAGES ON GEL HELP WANTED - (reply: 3)
     
643. how to purify plasmid and genomic dna - (reply: 3)
     
644. Answer for how to reuse DNA spin columns.. - (reply: 47)
     
645. Buffer BPTE for DNA electrophoresis - (reply: 3)
     
646. How to prepare DNA molecular weight ladder - Ladder and loading dye (reply: 13)
     
647. DNA degradation after plasmid mini preps - (reply: 6)
     
648. My DNA shoots out of the wells in my Agarose Gel... why? - Agarose Gel Loading Help (reply: 14)
     
649. Allele-specific DNA methylation - What to do if the discriminating SNP is a T/C ? (reply: 5)
     
650. DNA preparation for Chromatin Immuno Precipitation - (reply: 2)
     
651. DNA isolation from RLT buffer - (reply: 1)
     
652. DNase digestion step necessary for real-time RT-PCR? - (reply: 2)
     
653. running DNA on gel - (reply: 2)
     
654. Genomic DNA cutting - (reply: 12)
     
655. DNA conc for PCR - (reply: 1)
     
656. dna staining dyes - which DNA staining dyes do you use (reply: 4)
     
657. Is it posible to make ligation with DNA digested and stored at -20 - (reply: 2)
     
658. Extraction of genomic DNA from 10 fold serial dilution of cells. - (reply: 5)
     
659. alternative DNA staining dyes - which DNA staining dyes do you use (reply: 6)
     
660. DNA extraction from mouse ear punch - (reply: 2)
     
661. DNA extraction from rodent tails - (reply: 8)
     
662. improve the DNA cons. in RT-PCR - (reply: 2)
     
663. extracting DNA frm agarose gel no result - (reply: 3)
     
664. ChIP DNA yield problem - (reply: 2)
     
665. Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
     
666. digesting DNA with buffer incompatible enzymes - (reply: 4)
     
667. How to take picture of dna electrophoresis without a gel documentation unit? - (reply: 3)
     
668. gDNA contamination in cDNA library with cDNA creator smart kit - (reply: 1)
     
669. help needed in dna electophoresis - (reply: 7)
     
670. Synthetic DNA: anneal from Oligos or not? - Assemble from oligos or buy it outright? (reply: 20)
     
671. on-collumn DNASe in RNA purification - (reply: 3)
     
672. DNA sequencing company (cont.) - (reply: 3)
     
673. intrinsic fluorescence of DNA - (reply: 2)
     
674. Sequencing DNA - (reply: 2)
     
675. DNA SEQUENCING PROTOCOL - (reply: 3)
     
676. RNA transfection VS Plasmid DNA transfection - (reply: 3)
     
677. DNase - (reply: 1)
     
678. Recombinant DNA - (reply: 5)
     
679. long term strage of c DNA clones - strage of c DNA (reply: 3)
     
680. DNA markers - Gel run agarose or PAGE (reply: 1)
     
681. problem of DNA sonication in CHIP assay - (reply: 2)
     
682. DNA from slide mounted epoxy embedded fixed tissue - (reply: 1)
     
683. problems with purifying a 100 bp DNA fragment - (reply: 12)
     
684. Amplifying pathogen DNA in total DNA extractions - (reply: 2)
     
685. smear in miniprep DNA - (reply: 4)
     
686. Genomic DNA PCR vs plasmid DNA PCR - (reply: 6)
     
687. Need help evaluating apoptosis data - detection of degraded DNA in cytoplasmic fraction (reply: 5)
     
688. Interaction of Glucose with plasmid DNA - (reply: 3)
     
689. Extract DNA from dead bacteria - (reply: 4)
     
690. A basic question about DNA extraction - (reply: 2)
     
691. DNA from somatic tissues for bisulfite treatment - (reply: 3)
     
692. How to generate linear unidirectional tandem repeats of 100bp DNA? - Molecular Biology (reply: 14)
     
693. Difference between Extraction of DNA from chromosomal DNA and plasmid DNA - (reply: 1)
     
694. DNA extraction- bacterial cells - kits - PLease help!!! (reply: 2)
     
695. What's the difference between using DNA and RNA polymerase for PCR - (reply: 2)
     
696. RNAse and DNA digestion in Southern Blot - (reply: 3)
     
697. Nanodrop question - Spectrophotometrical measurment of bisulfite treated DNA (reply: 15)
     
698. PCR amplification of large piece from Bissulfite treated DNA - (reply: 2)
     
699. DNA extraction from saliva samples - (reply: 3)
     
700. RNase and DNase free? - water (reply: 2)
     
701. BAC DNA restriction analysis - I need help :( (reply: 4)
     
702. help chosing a fluorescent DNA stain - (reply: 3)
     
703. Uncut plasmid DNA - (reply: 6)
     
704. analysis of DNA using BamH1 and agarose gel electrophoresis - interpretation of results! (reply: 5)
     
705. DNA isolation kits - (reply: 4)
     
706. cloning, got conoly, no dna at all. help! - (reply: 9)
     
707. What do you mean by other letters aside from A, C, G, and T in DNA sequencing r - (reply: 3)
     
708. DNA purity - 260/230... (reply: 7)
     
709. Plasmid DNA Isolation problems, contaminating chromosoma DNA - How to isolate large inserts by miniprep and without chromosomal smearing (reply: 4)
     
710. genomic DNA contamination in plasmid DNA prep - (reply: 1)
     
711. DNA linear transformation failure - (reply: 2)
     
712. Analysis of DNA fingerprints-software - Image interpretation, bands size,... (reply: 2)
     
713. How do you work out a restriction digestion map for a circular DNA plasmid? - (reply: 2)
     
714. Can any one help me with restriction digestion map for DNA? - (reply: 3)
     
715. What factors affect the separation and visualisation of DNA fragments in gel ele - (reply: 1)
     
716. Maximun UV absorbance for DNA - (reply: 1)
     
717. some formulas - convert ng DNA/RNA/ml into copies/ml (reply: 3)
     
718. Primers for BS treated DNA - (reply: 5)
     
719. T4 phage DNA isolation protocol - (reply: 1)
     
720. Plasmid DNA quality for transfection - Is CsCl prep ever required? (reply: 3)
     
721. can someone tell me about extraction DNA from polyacrylamide gel? - (reply: 10)
     
722. genomic DNA is getting degraded while isolation - (reply: 2)
     
723. Techniques to isolate large size DNA - large size DNA purification from mixture with small size DNA (reply: 4)
     
724. Resuspended DNA in false buffer! - How to precipitate? (reply: 5)
     
725. degraded DNA in PFGE - (reply: 6)
     
726. In DNA gel electrophoresis, w