Protocol Online logo
Top : Forum Archives: : DNA Methylation, Histone and Chromatin Study

DNA methylation direct kit - (Jul/16/2007 )

Hi, did somebody use the new direct methylation kit from Zymo?
I use it and it worked perfectly, but i would like to have more advice on it.

-ptitced-

QUOTE (ptitced @ Jul 16 2007, 02:20 PM)
Hi, did somebody use the new direct methylation kit from Zymo?
I use it and it worked perfectly, but i would like to have more advice on it.


My colleague signed up to participate for one of these but hasn't received one yet (out of stock I think she was told). I'm hoping to try one of these too. I'm aware of another kit like this by Epigentek (epiquik whole cell bisulfite modification kit) -- if anyone have any expertise to compare these two, i'd like some info too.

also how many starting cells did you use ptitced?

Thanks

-Geno Man-

QUOTE (Geno Man @ Jul 25 2007, 09:42 AM)
QUOTE (ptitced @ Jul 16 2007, 02:20 PM)
Hi, did somebody use the new direct methylation kit from Zymo?
I use it and it worked perfectly, but i would like to have more advice on it.


My colleague signed up to participate for one of these but hasn't received one yet (out of stock I think she was told). I'm hoping to try one of these too. I'm aware of another kit like this by Epigentek (epiquik whole cell bisulfite modification kit) -- if anyone have any expertise to compare these two, i'd like some info too.

also how many starting cells did you use ptitced?

Thanks


We've gone down to about 1000 cells, with only 10% of those 1000 being methylated (ie 900 unmethylated cells, 100 methylated cells). Eluted in about 15ul and ran 3ul (probably overkill) for the Methylated reaction (p16 MSP primers)

-mawst95-