DNA laddering - apoptosis - any suggestion about the extraction procedure ? (Oct/09/2008 )
I've just posted a topic on the cell biology forum...I'm a very beginner on DNA extraction.
In few words, I'm currently using the Wizard kit from Promega (find the attached protocol) to extract DNA which requires
Should I use a different kit made expressely for fragmented DNA? If DNA is correctely fragmented, would it be easy to see ladder? Or are there some other tricks I should know to see any result?? Up to now, in the same experiment I have
3. big gDNA band
5. big gDNA band
Where 1 is control, 3 and 5 positive controls for apoptosis, 2 and 4 positive controls for apoptosis at lower apoptotic agent concentration...
Thank you!! I'm surrounded by MDs, nobody knows anything about molecular biology!!
Did you ever try Trizol? Or home made Phenol-Chlorophorm extraction?