DNA PAGE - suitability of PCR buffer (Jan/05/2009 )
I am using Promega's GoTaq Green PCR buffer (5X) for my PCR reactions. the reactions are working fine on agarose gel. Now I want to run these reactions on PAGE (Denaturing), can anybody tell whether i can use same buffer for PCR reactions to be run on PAGE? what is the compatibility?
I don't think it should make a difference, I did run it once and it ran fine but i was using a completely different enzyme and also the buffer was different.
However, if your sample is precious, just precipitate the DNA (3 hours) or colum purify (15 minutes) and get the sample in water and then run.
Hope it helps.