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Agilent Bioanalyzer vs Nanodrop DNA quantitation - DNA quantitation (Oct/24/2007 )


Has anyone checked whether dsDNA concentrations given by the Agilent Bioanalyzer 2100 and Nanodrop OD absorbance are consistent?
I am trying to quantitate 10 kb dsDNA fragments.




i did that for my RNA... use both Bioanalyzer and nanodrop.
both give me DIFFERENT concentration. surprisingly, bioanalyzer concentration sometimes less by half!!!
i asked some technical people, they said use 1.5ul instead of 1ul for nanodrop. it would b more accurate. Both bioanalyzer and nanodrop has its range, do not exceed the range when you load your sample into them.

however, it did improved the quality of whatever i read, but the concentration problem still the same. so, in the end, i only stick to nanodrop to calculate the concentration when i do dilution. so, stick to either bioanalyzer or nanodrop.


i use agilent bioanalyzer 2100 and nanodrop before to determine the quality of my RNA...
nanodrop jus gv the concentration, A260/A280, A260/A230...
but from agilent bioanalyzer 2100, we can get more info about the quality of RNA...

but using the agilent bioanalyzer is more troublesome compare to nanodrop...
fast and easy...


As long as you're not using it for any publishable results, it doesn't really matter which one you use, as long as you are CONSISTENT in choosing which one you use. Even if one gives half the reading of the other, by choosing to use one of them all the time all the measurements of your samples are in proportion with and comparable to one another.