How to sepate two similar sized DNA fragments - (Oct/09/2006 )

I want to separate two DNA fragments; one is 600bp the other is 617 bp can this be achieved with a high percentage agarose gel, i.e 3%

You can probably see the difference if you run two lanes next to one another, but I doubt if you could, for example, isolate one band from a pair by cutting.

I agree, I think you would have trouble isolating one band from the other in large quantities. Is it from a plasmid? Maybe you could amplify the fragment by PCR.

Hi,

If you want to physically separate them, instead of visually separating them, try cloning.

As for the latter, I'm not sure if 3% agarose will be able to separate them but phage434's idea is good, unless you don't have the nt sequence info to refer.

Bests