DNase I Amplification grade and DNase I for RNA treatment - (Mar/02/2006 )
Please I would like you provide with details about the use of DNase I Amplification grade and DNase I (INVITROGEN) to treat total RNA prior to RT-PCR reactions. I wonder if the DNase I (no amplification grade) is reliable for RNA purification for subsequent use for cDNA synthesis without affecting RNA integrity. Which is the difference between these enzymes?
Thank you very much,
For DNase treatment of RNA, it is critical that the DNase doesn't contain any RNase activity. So high purity and high grade DNase I is preferred.