RNA contamination: how to get ride off it from extracted DNA? - (Jun/18/2007 )

Greetings,

I checked the purity of DNA on 0.8% agarose and I found that the it is contaminated with RNA. I have RNase that came with different protocol (promega) can I use this one to get ride off the RNA?

Is it possible to get ride off RNA from already extracted DNA? How?

Regards,
Peace,

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check the purity of DNA using OD 260/280 too. if it contaminate with RNA then you will get the value under 1.7
well if you see RNA in you agarose gel ofcourse you could do a RNase treatment...
add sufficient amount of RNase in your sample and incubate it 37c for 30 min to 1 hour and check again in agarose
if the RNA vanish then you can proceed further with your sample

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Yes, you can use RNase from different company. Once after RNase treatment, you have to clean up as well. Normal phenol chloroform method perhaps.

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