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Genomic DNA hybridization for species identification - (Apr/22/2007 )

Hi guys,

I need some quick method, non radioactive if possible, to make a genomic DNA hybridization. The idea is to obtain genomic DNA from two different strains and to know quickly how much homology they share. This is the classical Cot experiment, but I would like to do it without radioactivity if it is possible. There are kits for everything today, but I can't find something for this.

Many thanks!



That's because very few people do this any more. The measurement is pretty crude, and the results are not very definitive. Here in the future, most people choose a few standard genes and sequence them. I do think it would be pretty easy to substitute fluorescence for radioactivity if you really want to do it.


agree with phage434, try doing fluorescence