How to prepare hemi-methylated DNA ? - (Jun/02/2006 )
GAT CGC CGA TGC GCG AAT
CTA GCG GCT ACG CGC TTA
I want to prepare this oligonucleotide. Is any methylase suitable for prepare this?
Thanks a lot !!
there are oligo synthesizing companies that would can make such oligos, (I just checked Invitrogen where I get all my oligos and they don't have any) you should google a company.
I would make both strands and then mix them together to get your DNA.
I am not aware of an enzyme that could perform a strand specific methylation that would have to be dependent on your sequence as well.