Why to use TE buffer for DNA resuspension - (Aug/22/2006 )
Why should i use TE-buffer for resuspension of my DNA.
I heard about two theries
1) DNA is more stable at the pH
2) DNAses are deactivated because of missing bivalent cations (because of the EDTA)
i tried to find papers for both theories, but i was not able to find something,...
Both are correct, pH is slightly basic therefor DNA is more easily dissolved than in acid environment, and less prone to acid hydrolysis (for this reason Tris is often used instead of water).
EDTA chelates ions indeed preventing degradation by nucleases.
in the absence of divalent cations at acidic pH.
OK, its at acid pH, but it says that the absence of bivalent cations activates DNAse. I was not able to find a paper that says something contrary.