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positive control for DNA ladder formation - (Apr/30/2007 )

Hi frnds
I want to know which chemical can be taken as positive control for DNA ladder formation( apoptotic studies)? I m in search of some chem which can induce DNA fragmentation in human lymphocytes...
All replies r welcomed!!!!

-Poonam Singh-


I try to do the same, but in HeLa cells.. I know that positive control for Hela cells is Mg132 (inhibitor of proteasome)..

and I have a question: do YOu have good protocol for doing this? I tried few methods and I always got either ''snot'' or pure genomic DNA...

Thanks for the answer