DNA isolation - PBS wash (Jan/24/2008 )

why is it necessary to wash the cells with PBS??? i knows that it removes the contamination. but how do the chemicals in PBS buffer play a role in removing contamination?
thanks in advance
-methylase

-Methylase-

QUOTE (Methylase @ Jan 24 2008, 09:49 AM)

why is it necessary to wash the cells with PBS??? i knows that it removes the contamination. but how do the chemicals in PBS buffer play a role in removing contamination?
thanks in advance
-methylase

I think PBS is used to don't affect the cells that much. PBS has salt concentrations that allow to maintain the tonicity of the cells.

-dinolx-

PBS is saline. It is used because its an isotonic solution.

-scolix-