Agarose gel concentration for genomic DNA - (Oct/19/2005 )

Why agarose gel 1.5% are suitable to DNA genome electrophoresis? Someone please answer me.....

Do you want to see if there is genomic DNA in your sample? I regularly use 1 % agarose to check that a genomic extract has worked. I don't see a problem with a 1.5% agarose gel except that it may migrate a little less down the gel.

Why agarose gel 1.5% are suitable to DNA genome electrophoresis? Someone please answer me.....

A more general answer is that higher percentage agarose gels have a higher degree of separation of lower molecular weight DNA, whereas lower percentage agarose gels separate higher molecular weight DNA more efficiency. This has to do with the migration of the different sized DNA through agarose molecules in the gel.

QUOTE (shilashah @ Oct 20 2005, 08:43 AM)

Why agarose gel 1.5% are suitable to DNA genome electrophoresis? Someone please answer me.....

If you are going to perform Southern blotting, the percentage of agarose depends on the length of the fragments yoy are interested in: for 0.5-2 kb fragments 0.9% agarose. For larger fragments 0.7% agarose.