SmaI at 37degree - I cut DNA by SmaI at 37degree by mistake. (Nov/03/2005 )
just now i cut my sample by SmaI and incubated it at 37degree, when i was aware of that fact,it was 1.5hrs later,so , i could do nothing but run a gel to see how my poor sample was going on, but it gave me a single band which i expected to obatin,so i want to ask whether SmaI incubated at 37 is also cut well?
i use buffer 10 X T+ BSA, and in a total volume of 200 microlitre reaction system..
this is quite curious as NEB says that sma I has 50% activity and a half life of 15' at 37°
You're a lucky guy.