I kb DNA ladder - (May/31/2007 )
What does 1 kb DNA ladder mean. Its mean Its highest band is of one 1 kb.
It means its major increment is 1 kb. It may be something like that: 250 bp, 500 bp, 750 bp, 1 kb, 2 kb, 3 kb, 4 kb, etc.
can you please provide the name of manufacturar of 100bp DNA ladder that is common in use??
the bands make a ladder of 1kb. From neb it's 500bp, 1kb, 1.5, 2 3 4 5 6 8 and 10kpb
bright band is 3kpb.
The NEB 2 log ladder has the advantage of not having to think. It has 100 bp bands less than 1 Kb, 1200, 1500, and 1Kb bands above that to 10 Kb. Bands 1000 and 3000 are bright. 7000 and 9000 are omitted. Except for very short fragments, this always works.
I agree with phage434 100%.
In addition to that stated by phage434, I like it because each band is quantified and is helpful in assessing the DNA amounts on gel.
we use this ladder for small DNA fragment
Different company with different 1kb. Have to check their brochure or catalogue.
NEBs 2-log is also a ladder of choice in our lab.
Only for longer templates we use Lambda-HindIII digest, also from NEB, but it's not quantified.
Lambda-hind3 ladder is also quantified. We used to have one in my old lab which was quantified. I am not sure if it was from NEB or not.