DNA sequencing kit - USB T7sequencing kit (Oct/18/2005 )
I have to run a sequencing reaction with the same primer iI have used to do my primer extension reactions. I was thinking about using the T7sequencing kit from USB. Has anyone used it before. Did anyone have experience with sequencing or have recommendations for other kits. Please help.waiting for reply.
thanks in advance.
Hi I think I got the wrong name of the kit there
I was talking about the sequenase version 2.0 DNA sequencing kit...if anyone here have used it before..please share pros and cons of the kit if possible..or if any other kit used ...thanks
I assume that you are doing this radioactively. If so then the USB kit will be fine. If you want to avoid using radioactivity than you might want to have a look at an old paper of mine. It works well and is all non radioactive.
Optimized rapid amplification of cDNA ends (RACE) for mapping bacterial mRNA transcripts
Improved automated DNA sequencing traces
I should mention that the above technique was designed for bacterial transcripts, but it will work fine for eukaryotes too.