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Phages - How to digest lambda DNA (May/11/2004 )

Help needed!!
Who knows how to digest DNA eluted from phages with the Qiagen Lambda Midi kit?? All restriction enzymes I used, did not digest the eluted DNA!



May be your DNA is not CLEAN!!!!!!
If your DNA contain SDS or an other detergent, the enzymes not work!!

Resuspend the DNA in TE buffer and centrifugue in COLD (-4ÂșC), by precipitate SDS.

I think so

By and good luck