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Is it posible to make ligation with DNA digested and stored at -20 - (Jan/16/2007 )

I want to know if I can use genomic DNA digested and plasmid DNA digested and stored at -20ÂșC for a long time to make a new ligation.


we have most of our plasmids in -20C and thaw them when we need to use it for any clonign steps. We also have the digested frgametns and/or plasmids in -20C and use it for ligations. I would typically warm the DNA fragments to 60C for 2 min. before using it for ligations.


we also keep all our daily used sample in -20c.
we preserve the stock in -80

i keep mini prep sample on my desk tongue.gif

-T. reesei-