Transformation of Yeast DNA into E.COli - (Mar/20/2005 )
Hello. I just want to know any more techniques on how to successfully get transformant s from yeast plasmid DNA transformed woth E.coli strain XL-1Blue. I have tried the Clontech protocol and after several performing the procedure several times already, i still don't get transformants...thanks
-bubblebath-
QUOTE (bubblebath @ Mar 21 2005, 12:02 AM)
Hello. I just want to know any more techniques on how to successfully get transformant s from yeast plasmid DNA transformed woth E.coli strain XL-1Blue. I have tried the Clontech protocol and after several performing the procedure several times already, i still don't get transformants...thanks
hi
please be more specific. do u mean that you are not able to transform yeast plasmids into ecoli or is that you are not able to get plasmids from yeast for transfrmation into ecoli?
-Maitreyis-