Alternative to phenol-chloroform purification of DNA - Genomic DNA purification (Jun/10/2005 )

Dear All,

I was wondering if someone could recommend an alternative method to phenol-chloroform purification of genomic DNA.

I need to get genomic DNA as pure as possble and I tried using phenol-chloroform. It produced very pure DNA but however any phenol or chloroform that get carried through degrade the dyes in subsequent labelling reactions.

Any suggestions will be appreciated.

Many thanks,
G'Eye

-GoldenEye-

I have found the chargeswitch product by invitrogen superb in both yield and quality.

-u03cka-

I have done the phenol-chloroform method and using a commercial kit from Qiagen.
The Qiagen kit is definitely better.
good luck
p..

-Pria-

Yah, quiagen kits kick ass when it comes to purifying dna.

-pBluescript-

Dear GlodenEye,

Can you tell me what organism that you dealling with?
I have several protocols (not for plant extraction) which might help in you project.

Best regards
Hadrian.

-Hadrian-