DNA extraction problem - CTAB solution (Jun/22/2006 )
Hi,
It may be very trivial, but it's my first experience with DNA extraction. I'm using Steenkamp solution (a kind of CTAB) and when I add the solution to the ground leaf sample, it makes a ball and doesn't dissolve (like glue). I replaced the solution with usual CTAB solutions which appear on internet protocols. for the new one I got these figures from nanodrop. 260/280= 2.04, 260/230=2.78. for the second one I don't know if it's valid and should I work on all the samples. this figure seems too high (compared to 1.8 which mostly referred to).
Thanks
Labserve
-Labserve-
QUOTE (Labserve @ Jun 22 2006, 01:37 PM)
Hi,
It may be very trivial, but it's my first experience with DNA extraction. I'm using Steenkamp solution (a kind of CTAB) and when I add the solution to the ground leaf sample, it makes a ball and doesn't dissolve (like glue). I replaced the solution with usual CTAB solutions which appear on internet protocols. for the new one I got these figures from nanodrop. 260/280= 2.04, 260/230=2.78. for the second one I don't know if it's valid and should I work on all the samples. this figure seems too high (compared to 1.8 which mostly referred to).
Thanks
Labserve
It may be very trivial, but it's my first experience with DNA extraction. I'm using Steenkamp solution (a kind of CTAB) and when I add the solution to the ground leaf sample, it makes a ball and doesn't dissolve (like glue). I replaced the solution with usual CTAB solutions which appear on internet protocols. for the new one I got these figures from nanodrop. 260/280= 2.04, 260/230=2.78. for the second one I don't know if it's valid and should I work on all the samples. this figure seems too high (compared to 1.8 which mostly referred to).
Thanks
Labserve
hello
i'm a bit confused wit wat u've written,u're adding the CTAB to the ground leaf samples?? y dont u try homogenizing the leaf samples using the CTAB buffer?? or does it mean something else?
anupama
-anupama-
have you done a phenol/chloroform extraction to make sure there are no more proteins? A little RNAse might help too.
-perneseblue-