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DNA extraction problem - CTAB solution (Jun/22/2006 )

Hi,
It may be very trivial, but it's my first experience with DNA extraction. I'm using Steenkamp solution (a kind of CTAB) and when I add the solution to the ground leaf sample, it makes a ball and doesn't dissolve (like glue). I replaced the solution with usual CTAB solutions which appear on internet protocols. for the new one I got these figures from nanodrop. 260/280= 2.04, 260/230=2.78. for the second one I don't know if it's valid and should I work on all the samples. this figure seems too high (compared to 1.8 which mostly referred to).
Thanks
Labserve

-Labserve-

QUOTE (Labserve @ Jun 22 2006, 01:37 PM)
Hi,
It may be very trivial, but it's my first experience with DNA extraction. I'm using Steenkamp solution (a kind of CTAB) and when I add the solution to the ground leaf sample, it makes a ball and doesn't dissolve (like glue). I replaced the solution with usual CTAB solutions which appear on internet protocols. for the new one I got these figures from nanodrop. 260/280= 2.04, 260/230=2.78. for the second one I don't know if it's valid and should I work on all the samples. this figure seems too high (compared to 1.8 which mostly referred to).
Thanks
Labserve

hello
i'm a bit confused wit wat u've written,u're adding the CTAB to the ground leaf samples?? y dont u try homogenizing the leaf samples using the CTAB buffer?? or does it mean something else?
anupama

-anupama-

have you done a phenol/chloroform extraction to make sure there are no more proteins? A little RNAse might help too.

-perneseblue-