Problems with PCR Purification - When I try to purify my PCR product I lose all of my DNA? (Jan/26/2002 )
I have been trying to purify PCR products with sizes ranging from 150-250bp, to be used for sequencing and cloning. I've been using the Qiagen QIAquick PCR Purification Kit, but everytime I lose nearly all my DNA. Can anyone suggest a remedy?
I have used Qiagen PCR kit and Gel purification kit for a while and never have this problem. If my pcr gives me sharp band w/o primer dimer, I would use pcr kit, if not, then gel kit. I usually use 20-30 ul of EB buffer for the final elution, which will give me higher concentraction of DAN.
Use qiagen pcr kit, but elute with 10-15ul, if you have smaller fragments, it will works fine.
Why not you try and use the eppendorf 5 prime Perfectprep PCR gel cleanup. I works really well
Why not you try the eppendorf 5 prime Perfectprep gel cleanup kit. It works really well