DNA isolation: RNase A or B - which use? - (Oct/17/2006 )

Hi,
I want to isolate DNA from fungi (Blumeria graminis - powdery mildew) and in protocol is used RNase B (Sigma) to digest RNA. I would like to work with RNase A which I have to disposal, but Im not sure if it is correct. RNase B has similar protein structure, the same specifity... Does anybody have experiences with RNase A and B treatment?
Thanks.

-Myval-

i've only ever used RNase A but - i don't work on fungi! perhaps there is a specific reason for this choice? Could you call Sigma to ask their tech support?

-aussieuk-

QUOTE (aussieuk @ Oct 24 2006, 05:22 AM)

i've only ever used RNase A but - i don't work on fungi! perhaps there is a specific reason for this choice? Could you call Sigma to ask their tech support?

You can use RNase A. There are numerous fungal DNA kits using RNase A, plus like you said RNase B has the same specificity as RNase A

-Bachelor-

So why nature made evolve both RNAses? different but the same?

QUOTE (Bachelor @ Oct 24 2006, 05:03 PM)

QUOTE (aussieuk @ Oct 24 2006, 05:22 AM)

i've only ever used RNase A but - i don't work on fungi! perhaps there is a specific reason for this choice? Could you call Sigma to ask their tech support?

You can use RNase A. There are numerous fungal DNA kits using RNase A, plus like you said RNase B has the same specificity as RNase A

-tertu-