Produce DNA ladder marker - (Jul/16/2007 )
How about producing DNA ladder marker?
I would like to make a kind of DNA ladder marker, which will just contain my interesting DNA binds and also can detect the concentration of my samples!
-ffyy-
QUOTE (ffyy @ Jul 16 2007, 05:55 AM)
How about producing DNA ladder marker?
I would like to make a kind of DNA ladder marker, which will just contain my interesting DNA binds and also can detect the concentration of my samples!
I would like to make a kind of DNA ladder marker, which will just contain my interesting DNA binds and also can detect the concentration of my samples!
How about just buying them, makes its simpler.
-scolix-
QUOTE (ffyy @ Jul 16 2007, 07:55 PM)
How about producing DNA ladder marker?
I would like to make a kind of DNA ladder marker, which will just contain my interesting DNA binds and also can detect the concentration of my samples!
I would like to make a kind of DNA ladder marker, which will just contain my interesting DNA binds and also can detect the concentration of my samples!
Some companies do make them according to your DNA size and different concentrations. I think one of them is NEB. But I always dont think to quantify it using gel. Should use at least the absorbance readings.
-timjim-
QUOTE (timjim @ Jul 17 2007, 08:14 AM)
QUOTE (ffyy @ Jul 16 2007, 07:55 PM)
How about producing DNA ladder marker?
I would like to make a kind of DNA ladder marker, which will just contain my interesting DNA binds and also can detect the concentration of my samples!
I would like to make a kind of DNA ladder marker, which will just contain my interesting DNA binds and also can detect the concentration of my samples!
Some companies do make them according to your DNA size and different concentrations. I think one of them is NEB. But I always dont think to quantify it using gel. Should use at least the absorbance readings.
In some of the ladders (for eg. Lambda Hind3) the bands are also quantified so its useful for concentration estimation.
-scolix-