Chaotropic Salts for DNA Extraction - (May/09/2002 )
I have been successfully extracting plasmid fragments from 2mls of FCS by using Qiagens Gel Extraction Kit by adding the required 6mls (3vols) of buffer QG. This is proving expensive and I would like to replace the QG with a another chaotropic salt solution. Has anyone had success using KI solutions with the kit? Can anyone suggest another salt.
Thanks for your help,
Sorry. I meant NaI in the last post.
You can try Guanidine HCl. It's a pretty potent chaotrope.