Storing low conc plasmid DNA - (Aug/12/2005 )
I need to store serial dilutions of plasmid DNA and want to know the best way to store the lowest dilutions to stop them disappearing in the tube?! I'm thinking that I can maybe add some random DNA like salmon sperm? I'm already using low DNA binding tubes.
any bright ideas?
I am not sure how low you are trying to go, but i have kept dilutions as low as 0.7fg/ul and seen consistent pcrs a year later. Maybe it is not necessary to add anything?? (Mine were stored at -20C non-frost free)
Use TE and BSA.
DNA sequencing programs
i'ver diluted in water a plasmid at 10ng/µl and it's still ok after months.