dna isolation help! - (Sep/27/2006 )
I've come to my wit's end over a seemingly very small problem. please help!!!!.
I am doing dna extraction from mouse and hamster tissues. some of my samples are showing very good 260-280 ratio, however some of them (include both hamster and mouse tissues) show very poor ratios 1.6-1.65 even after repeated chisam purification. I changed the d/w, helped a little. but not much (ratios changed from1.5 to 1.6).
i am using water for dissolving. i have also tried dissolving in more volume in order to eliminate solubility problems. I've used same reagents for all the samples.
can this happen with degraded samples? hope not.
have a look at this: