freeze drying of genomic DNA - i want store The DNA genomic for many years what is the butter manner (Nov/27/2007 )
Hi All,
I want store The genomic DNA for many years, what is the butter manner, it is possibe to freeze dryer the DNA without any genetic affect
thank for your responses!
-wissam-
DNA molecules are quiet stable. As long as you control the pH to neutral, Ca2+ and Mg2+ free (with added EDTA), under -20C you can keep it for long time. Ethanol precipitation is better and DNA is easier to reconstitute to solution than dried samples.
-genehunter-1-
I agree with genehunter. Store DNA in TE at -20C. This should keep your samples for generations to come.
-scolix-
can't be certain about generations , but if the DNA made by my supervisor is any indicator, it'll last most certainly two, even three decades
-perneseblue-