Types of DNA polymerases that you use. - (Nov/18/2005 )
Hello all, I have already asked this question before but since im very new to PCR i wanted to see more choises.
I m choosing DNA polymerase for PCR of 1791 bp protein (GC 55.89%) and im adding RE sites at the end of each termini.....what type of DNA polymerase would you recommend...thanx a lot 
I m choosing DNA polymerase for PCR of 1791 bp protein (GC 55.89%) and im adding RE sites at the end of each termini.....what type of DNA polymerase would you recommend...thanx a lot
Hi Kathy,
if its important, that your sequence is 100 % correct, i would take the Phusion polymerase. it has a proofreading function and it´s very fast. i think it´s the best you can get, but it´s a little bit more expensive
Good luck Delfe
Not a single polymerase is 100% error free. So, you check out suppliers websites (NEB, roche, invitrogen, qiagen, novagen, stratagene, promega, clontech, takara...) and see how much you can spend on it, and how low your want your expected error rate.
And when you choose one, it's still trial and error for your PCR, I know that on this board I'm one of the few that's for instance getting good PCR amplification when using platinum pfx (from invitrogen)...
I posted an answer in your original thread here. Your PCR product is fairly small; I rarely find errors in products of comparable size, regardless of whether I use standard Taq or a high fidelity Taq (or a polymerase from another species, or a mix). Still, my choice of polymerase is determined by my intentions for the product.
BTW, I know this is picky, but there's no such thing as a "1791 bp protein"...