RNA sample precipitates when I add the 10X Buffer of the Turbo Dnase (AMBION) - RNA precipitation (Nov/20/2008 )
HI There, this is driving me crazy. I purify RNA from my bacteria with trizol and at the end of the purification the A260/280 and 260/230 are great appx 2.0 However when I add the 10X Buffer of the Turbo Dnase (AMBION) the RNA sample precipitates no matter what!!!!!!. Why????????
Has anyone had this problem?. It is only when I add the 10X Turbo DNAse Buffer......Help. Tomas
too concentrated maybe? I don't understand why the RNA should precipitate like this. Have you tried contacting ambion? We have no problems with this kit in human, mouse and rat rna