DNA Multimers ... - Things not happening as it should be ... (Jan/26/2005 )
I have an oligo (A) (non-phosphorylated in 5') of ~ 100 bases.
I have its complement ( with two over hangs (~4 bases each) on both he sides(5' and 3').
the overhangs on the B are palindromic. as in the 5' overhang is palindromic to itself.
I annealed both of them.
phosphorylated the sample.
after ligation, when i ran it on the gel, i saw only monomers and dimers.
Why is this so ???
y not other multimers ???
did i go wrong sumwhere ???
I am not quite shure if I understand what you intended to do or wanted to see on the gel. But I suppose the ligation time was to short!?
What do you mean with Monomer: ds AB??