DNA / RNA extraction from freeze dried tissue - (Apr/06/2006 )
I was wondering if anyone out there had successfully used freeze dried tissue (esp. Arabidopsis) for extraction of DNA or RNA. I know this technique is used for extractions from fungal tissue.
Thanks for your time.
We regularly used to isolate gDNA from freeze dried plant tissue
The trick in getting high molecular weight DNA is to do the freeze drying quickly immediately after taking the tissue
Thanks for replying, John.
Did you use a standard genomic DNA prep (I normally use a CTAB buffer and phenol / chloroform)? Is it tougher to get rid of contaminating carbohydrates etc?
We used either a salting out prep based on the miller technique (similar to Gentra's puregene but homebrew), or a modified CTAB method
Polysaccharides can be a problem for some preps but with CTAB followed by phenol/chloroform you should be just fine
You won't get any useful RNA out though
Thanks. I had feared as much.