Protocol Online logo
Top : Forum Archives: : Molecular Biology

DIGĀ  DNA probe labelling kit - (Sep/27/2001 )

Dear Friends:I plan to use 500bp cDNA fragment from agarose gel to make probefor screening a genomic library.Do you suggest me to use DIG LABELLING ?Do you know which kit is good ?

please email to:shengwu99!




Dear Wu:

Use the kit from Roche Biomeolecular Sciences. It works well and is much better than radioactive methods.

HCMycoBact Experts, Inc.A Division of DSTF-Global


Are you going to use the DIG labelled probe on colony blots? I've had problems with non-specific binding on my colony blots. I used the DIG system from Roche.  However, it worked well with southerns