DIG DNA probe labelling kit - (Sep/27/2001 )
Dear Friends:I plan to use 500bp cDNA fragment from agarose gel to make probefor screening a genomic library.Do you suggest me to use DIG LABELLING ?Do you know which kit is good ?
please email to:shengwu99!yahoo.com
Use the kit from Roche Biomeolecular Sciences. It works well and is much better than radioactive methods.
HCMycoBact Experts, Inc.A Division of DSTF-Globalhttp://www.dstf.bigstep.com
Are you going to use the DIG labelled probe on colony blots? I've had problems with non-specific binding on my colony blots. I used the DIG system from Roche. However, it worked well with southerns