Anyone knows the role of polyadenosine in DNA extractions? - (Mar/28/2006 )
Does anyone know why add PolyA when extract DNA?
it can work sort of like a carrier, and make it easier to purify DNA if you expect a wimpy yield
does this help?
I added polyA in the lysis buffer at the start of the extraction. Do you know the chemistry of this reaction, or any details, or where I can find out about this? Google gave me lots of stuff about mrna polyA tail, and nothing about DNA extraction.
do a google with search terms
"polyA extraction dna"
and you will get some hits, that will hopefully help you