polyacrylamide gel for DNA analysis - (Oct/03/2007 )
Hi, i need to separate two DNA bands, of size 150 bp and 160 bp. I'm planning to use polyacrylamide gels after several failed attempts with agarose gels. I need the recipe of this gel and electrophoresis condition. Thank you!
Do you know Google? There you find almost everything.
Are you sure you can't cut one but not the other with a restriction enzyme? That would make it trivial.
Yes I have heard of Google, and I have also heard of Bioforum. Between these two resources, I believe the latter will provide a faster and more reliable answer. Thanks for the links, they are great!
The two bands which I want to separate is actually products of restriction digestion and their visualisation is very critical. Hence, I cannot cut one of them into even smaller fragments.
Another question. In electrophoresis, what does 1 V/cm mean? Does it mean for a gel with length (in the direction of band migration) of, for example, 15 cm, I should use 15 V?
Generally for a 5% polyacrylamide gel, what voltage should I use to separate my dsDNA of sizes 150 bp and 160 bp?