Genomic DNA purification - Very low 260/280 or <0.01ug/ml!!! (Dec/29/2004 )
Warm Christmas Wishes and Happy 2005 to ALL!!
This time, I have a set of Genomic DNA samples purified by QIAmp DNA Blood Mini Kit according to manufacturer's instructions. In some samples the 260/280 ratios are very low (some 1.26- some 0.88), in all the concentration os <0.01 ug/ul...
The company's troubleshooting is not very useful due to the fact that Protease is stored in aliquots, 100% alcohol was used, > 1 million cells were used for extraction, etc, etc, and more importantly, the sources of these samples are no longer available!! so this is ALL or NONE...
I was thinking in re-extract DNA by using this kit or speed-vac concentration...
What do you think/suggest?
I will REALLY appreciate you responses!!
Spectro analysis for genomic DNA is not always thrust worthy.
What is the purpose of the DNA you isolated?
I had a similar problem. So precipitated the samples by using standard protokoll (NaAc and EtOH). But what are you using th samples for?
The second time I did no OD measuring, and the real time pcr made no problems though. So it depends on the application.
thanks for your replies...
DNA will be used for PCR amlification.