suggestion for purchasing DNA quantification standards - (Nov/14/2005 )
Can anyone suggest a good company selling standard DNA for DNA quantification? I am not very confident of the scale we have in the lab, so am a bit concerned of the accuracy. Thanks.
lambda hindIII is usually pretty reliable if your DNA is good and your RE works...
NEB, Promega, Invitrogen, Sigma, Fisher, who doesn't sell them?
NEB, Promega, Invitrogen, Sigma, Fisher, who doesn't sell them?
Thanks for the reply, but I'm trying to quantify total genomic DNA and is looking for a premade DNA standard. I don't think the DNA marker will do me any good though.
yeah, but if you purchase the standard, can't you use it for the same purpose? You are given a specific concentration of DNA in several lengths of pieces that you can dilute and spec
I don't know if perhaps this will not work for you, but when I need a good quantification standard for my spec, either DNA or RNA, I get a little standard and make a few fresh aliquoted dilutions, and compare expected vs actual. then I'm only buying one reagent for both purposes
however, my needs are usually not too picky and maybe this wont' work for you.
sorry! 
What about buying some salmon sperm or calf thymus DNA, and resuspending it to an appropriate concentration?