DNA extraction from Fruit pulp - HELP !!!! (Nov/10/2008 )
Anyone doing DNA extraction from fruits pulp ?
What protocol does you all use/??
How to get rid off high viscosity pellet?
THank you very much ...
-libraryguy-
QUOTE (libraryguy @ Nov 10 2008, 09:09 PM)
Anyone doing DNA extraction from fruits pulp ?
What protocol does you all use/??
How to get rid off high viscosity pellet?
THank you very much ...
What protocol does you all use/??
How to get rid off high viscosity pellet?
THank you very much ...
I'd suggest you try AquaRNA. Add 1 volume of AquaRNA solution to your fruit pulp, homogenize, spin down debris, precipitate DNA/RNA from the supernatant with either isopropanol or AquaPrecipi, if isopropanol precipitation gives you too much fruit pigments.
-chessplayer-
is there anyway we can use the conventional way to extract from it ?
My problem here is the highly viscousity of the pellet..
Sorry but I will need time to order that AquaRNA.
-libraryguy-