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which kit for blood DNA isolation is better - (Oct/26/2006 )

hi people, i've tried isolating dna and detecting several genes by multiplex before. I used a simple conventional kit that time however, I find whatman FTA technology quite intersting.

I would like to try out this product, because i need to get blood sample from children ( youknow how difficult it is) so i need a small amount of blood sample as possible ( just a finger prick). I was thinking what will you choose between these two whatman products:

http://www.whatman.com/repository/document...pin_FINAL_A.pdf
or
http://www.whatman.co.jp/product/Bio/ftalist/fta-pdf/01.pdf

I just need a small amount of DNA for mulltiplex PCR from a small amount of blood sample.

If i were you, what will i purchase?

By the way, my funding is limited,all i need is DNA sample.
thanks

-rina77-

hi
i don't use these but i would buy the first one which seems cleaner for preparations
the second one imply to cut and move sample... not really good.

-fred_33-

Have you considered buccal swabs rather than FTA paper? they are much less invasive

-John Buckels-

thank you all for your comments. I was also thinking of buccal swabs but my problem is i cant immediately process the samples and isolate dna from it immediately because i will collect it far from my lab and transport it back to lab after several days.

can you recommend a kit or any advise on how can i obtain small amount less invasive means of getting sample,transporting it and isolating dna later? thank you.

-rina77-

buccal swabs would be fine for this application in my experience.

-John Buckels-