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DNA sequencing - (Apr/16/2008 )

When you run the gel in dideoxy method you start to read the band from down to up. It will give you the squence of 5'-3' DNA????


It will give you sequence in the 5' to 3' direction of the primer being used (your statement is ambiguous since it would be 5' to 3' on one or the other of the strands).