Measuring DNA concentration without spectrometer - (Mar/31/2006 )
Hi,
I would like to know if there is any way to adjust DNA concentration obtained by boiling without a UV spec.
-microcharm-
You can run it on a gel against a mass ladder. Many DNA ladders are quantitative. Check with the manufacturer's instructions or manual for you ladder.
This is not very accurate, but comparing bands visually can give you a quick idea of whether your DNA extraction is better using one method or another other.
-vasussci-