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running DNA on gel - (Jan/22/2007 )

When i quantified DNA at 260nm the ratios appeared very high........more than 2.5. Why would this be?

Can I run an aliquot of this on an agarose gel to check the quality for PCR purposes? How much should I load?



I never get 260/280 above 2! and actually I had never heard of values as high as 2.5. But I got few links which mention similar observation. Check:


i usually get A260/A280= 1.8 to 2.2

i once got 2.4

u can try RNAse treatment and then check again

-T. reesei-