running DNA on gel - (Jan/22/2007 )
When i quantified DNA at 260nm the ratios appeared very high........more than 2.5. Why would this be?
Can I run an aliquot of this on an agarose gel to check the quality for PCR purposes? How much should I load?
i usually get A260/A280= 1.8 to 2.2
i once got 2.4
u can try RNAse treatment and then check again