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Plasmid DNA Purification - plasmid was contaminated by Genomic DNA (Jan/19/2005 )

hello, everybody.
i have tried three different kits for mini-prep plasmid DNA purification. but i could not get rid of the chrosomal DNA contamination though i strictly followed the manual of each producer.
could anyone give me any advice? thanks in advance. sad.gif sad.gif


Try to reduce the lysis time in step2. And do not extend 5min.