gDNA isolation kit? - (Aug/22/2005 )

Hi,

I'm thinking of switching from our current method of genomic DNA isolation on primary cell cultures to a kit based method and was looking for someones opinion on the promega line.

Are the wizard genomic DNA purification kit and the wizard SV genomic DNA purification kit fairly similar in terms of yields and quality? If they are I would lean towards the SV kit since it seems less time consuming, what do you think?

-CW

---

I've used both and each is quite effective, but you're right the SV kit is easy is use and much quicker. The SV kit is a little more expensive, but you save time and the DNA will be a good quality. Essentially the SV column is the Wizard 'glass milk' embedded onto a memberane in a column format. You also have the different options for easy purification - vac or spin...

I'm not sure what methods for purification you were using before, but you can't beat using kits these days. The competition in the area, DNA and RNA purification especially, is really getting fierce resulting in great products from the big guys (like Promega, etc) and small or new guys (like MN). As a researcher you should use this to your advantage and push for the best price. I guarantee over the long term your time and dollar savings will welcomed and surprising.

---

One thing to keep in mind with the wizard kits is they can shear your DNA a bit. This won't matter for most applications like PCR but if you need to make a large insert library then you may have problems.

Daniel

Software to improve DNA sequencing

---

The DNA will mainly be used in methylation analysis of small amplified regions.. < 500 bp so I don't think shearing will be too much of a problem.

---