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Elute DNA from Watmann paper - I received a plasmid in the mail and cant get transfectants (Sep/25/2007 )

Hello

I received a plasmid in the mail. It was blotted onto watman paper.
I cut a small piece of the area and incubated in water. Mixed and vortexed.
I tried to get transfectant E.Coly and did not get any colonies (antibiotic and positive controls check out Ok).
A friend suggested using Tris pH8.0 for the extraction.

Any suggestions?

Thank you all

-Alejandro-

see here and here where it was discussed.

-fred_33-

QUOTE (Alejandro @ Sep 25 2007, 09:39 AM)
Hello

I received a plasmid in the mail. It was blotted onto watman paper.
I cut a small piece of the area and incubated in water. Mixed and vortexed.
I tried to get transfectant E.Coly and did not get any colonies (antibiotic and positive controls check out Ok).
A friend suggested using Tris pH8.0 for the extraction.

Any suggestions?

Thank you all


elute the whole spot as most send only 1-10 µg; it does not matter if with water or TE

-The Bearer-