T4 phage DNA isolation protocol - (Nov/17/2006 )
Hi all,
I want to isolate the T4 phage DNA genome and then use it as template for PCR. Who tell me a T4 phage DNA isolation protocol?
Thank you very much!
Nam
-chukynam-
QUOTE (chukynam @ Nov 17 2006, 11:24 AM)
Hi all,
I want to isolate the T4 phage DNA genome and then use it as template for PCR. Who tell me a T4 phage DNA isolation protocol?
Thank you very much!
Nam
I want to isolate the T4 phage DNA genome and then use it as template for PCR. Who tell me a T4 phage DNA isolation protocol?
Thank you very much!
Nam
Just like any other lytic phage. Check protocols for miniprepping lambda.
For example http://www.nestgrp.com/protocols/nucleobon...lease_free.html
or http://www.scielo.br/scielo.php?pid=S1415-...ipt=sci_arttext
-lillymay-